LOCUS       sequence-41458-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Dower K, Rosbash M
  TITLE     T7 RNA polymerase-directed transcripts are processed in yeast and 
            link 3' end formation to mRNA nuclear export.
  JOURNAL   RNA. 2002 May;8(5):686-97.
  PUBMED    12022234
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2002-05"; volume: "8(5)"; pages: "686-97"
COMMENT     Sequence Label: pRS314-T7-GFP
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..18
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3..19
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(40..86)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(259..279)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctcgaggtcg acggtatcga taagcttgat atcgaattcc aaaaaacccc tcaagacccg
       61 tttagaggcc ccaaggggtt atgctatttc aatcaatgaa tcaaaatgtc attaaaatag
      121 tatataaatt gagactaagt cataaagcta taaaaagaaa atttatttaa atgcaagatt
      181 taaagtaaat tcacttagtc gacttagcag ccagatcctt tgtatagttc atccatgcca
      241 tgtgtaatcc cagcagctgt tacaaactca agaaggacca tgtggtctct cttttcgttg
      301 ggatctttcg aaagggcaga ttgtgtggac aggtaatggt tgtctggtaa aaggacaggg
      361 ccatcgccaa ttggagtatt ttgttgataa tggtctgcta gttgaacgct tccatcttca
      421 atgttgtgtc taattttgaa gttagctttg attccattct tttgtttgtc tgccatgatg
      481 tatacattgt gtgagttata gttgtattcc aatttgtgcc caagaatgtt tccatcttct
      541 ttaaaatcaa taccttttaa ctcgattcta ttaacaaggg tatcaccttc aaacttgact
      601 tcagcacgtg tcttgtagtt cccgtcatct ttgaaaaata tagttctttc ctgtacataa
      661 ccttcgggca tggcactctt gaaaaagtca tgccgtttca tatgatccgg gtatcttgaa
      721 aagcactgaa caccataagt gaaagtagtg acaagtgttg gccatggaac aggtagtttt
//
