LOCUS       sequence-41469-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, 
            Scharenberg AM
  TITLE     Tracking genome engineering outcome at individual DNA breakpoints.
  JOURNAL   Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.
  PUBMED    21743461
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-10- 10"; volume: "8"; 
            issue: "8"; pages: "671-6"
COMMENT     Sequence Label: pRRL.EF1a.HA.NLS.Sce(opt).T2A.BFP
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      117..126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             132..158
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             132..158
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             168..188
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
       61 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagga tctgccgcca
      121 ccatgggcgt atacccctac gacgtgcccg actacgcccc cgggccccct aagaaaaaga
      181 ggaaggtgaa gaacatcaag aagaaccagg tcatgaacct gggccccaac agcaagctgc
      241 tgaaggagta caagagccag ctgatcgagc tgaacatcga gcagttcgag gccggcatcg
      301 gcctgatcct gggcgacgcc tacatcagga gcagggacga gggcaagacc tactgcatgc
      361 agttcgagtg gaagaacaag gcctacatgg accacgtgtg cctgctgtac gaccagtggg
      421 tgctgagccc cccccacaag aaggagaggg tgaaccacct gggcaacctg gtcatcacct
      481 ggggcgccca gaccttcaag caccaggcct tcaacaagct ggccaacctg ttcatcgtga
      541 acaacaagaa gaccatcccc aacaacctgg tggagaacta cctgaccccc atgagcctgg
      601 cctactggtt catggacgac ggcggcaagt gggactacaa caagaacagc accaacaaga
      661 gcatcgtgct gaacacccag agcttcacct tcgaggaggt ggagtacctg gtgaagggcc
      721 tgaggaacaa gttccagctg aactgctacg tgaagatcaa caagaacaag cccatcatct
      781 acatcgacag catgagctac ctgatcttct acaacctgat caagccctac ctgat
//
