LOCUS       sequence-41471-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Kadener S, Stoleru D, McDonald M, Nawathean P, Rosbash M
  TITLE     Clockwork Orange is a transcriptional repressor and a new Drosophila
            circadian pacemaker component.
  JOURNAL   Genes Dev. 2007 Jul 1;21(13):1675-86. Epub 2007 Jun 19.
  PUBMED    17578907
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2007-07-1"; volume: "21(13)"; pages: "1675-86. Epub 
            2007 Jun 19"
COMMENT     Sequence Label: pAC-Luc
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..45
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(68..85)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(95..136)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 tcgaggctga tcagcgagct caggccttag aaggcacagt cgaggctgat cagcgggttt
       61 aaactcaatg gtgatggtga tgatgaccgg tacgcgtaga atcgagaccg aggagagggt
      121 tagggatagg cttaccttcg aaccgcgggc cctctagact cgagcgttac aatttggact
      181 ttccgccctt cttggccttt atgaggatct ctctgatttt tcttgcgtcg agttttccgg
      241 taagaccttt cggtacttcg tccacaaaca caactcctcc gcgcaacttt ttcgcggttg
      301 ttacttgact ggcgacgtaa tccacgatct ctttttccgt catcgtcttt ccgtgctcca
      361 aaacaacaac ggcggcggga agttcaccgg cgtcatcgtc gggaagacct gccacgcccg
      421 cgtcgaagat gttggggtgt tgtaacaata tcgattccaa ttcagcgggg gccacctgat
      481 atcctttgta tttaattaaa gacttcaagc ggtcaactat gaagaagtgt tcgtcttcgt
      541 cccagtaagc tatgtctcca gaatgtagcc atccatcctt gtcaatcaag gcgttggtcg
      601 cttccggatt gtttacataa ccggacataa tcataggtcc tctgacacat aattcgcctc
      661 tctgattaac gcccagcgtt ttcccggtat ccagatccac aaccttcgct tcaaaaaatg
      721 gaacaacttt accgaccgcg cccggtttat catccccctc gggtgtaatc agaatagctg
      781 atgtagtctc agtgagccca tatccttgtc gtatccctgg aagatggaag cgttttgcaa
      841 ccgcttcccc gacttctttc gaaagaggtg cgcccccaga a
//
