LOCUS       sequence-41473-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Kadener S, Stoleru D, McDonald M, Nawathean P, Rosbash M
  TITLE     Clockwork Orange is a transcriptional repressor and a new Drosophila
            circadian pacemaker component.
  JOURNAL   Genes Dev. 2007 Jul 1;21(13):1675-86. Epub 2007 Jun 19.
  PUBMED    17578907
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2007-07-1"; volume: "21(13)"; pages: "1675-86. Epub 
            2007 Jun 19"
COMMENT     Sequence Label: pAC-cwo
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     20..37
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(60..77)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(87..128)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gatcagcgag ctcaggcctt agaaggcaca gtcgaggctg atcagcgggt ttaaactcaa
       61 tggtgatggt gatgatgacc ggtacgcgta gaatcgagac cgaggagagg gttagggata
      121 ggcttacctt cgaaccgcgg gccctctaga ctcgagcggc cgccactgtg ctggatatct
      181 gcagaattcc accagctctg gttcatggcc tgctccagtt tggccttagc caccgctgcg
      241 gcggccacag cggctgcctg agccgccgcc gagttggtgg acagcggaag tcccgctccg
      301 gcggccacca cagcctgttg ttgctgttgc ttgccaacgg ccacggcggc ggctgcagca
      361 gcggccaaga gcgatgcgga cggtgaactg ggcatgaagt tcacgttgat gttgatgggg
      421 tgcactacag gcatgctggt gtaactcttc tcgagcagat ccataccgtt gaggaagggc
      481 accagtgcat tatagtccac gttcaggggc acgtagtagt tgccctgtcc gtgaagtgcg
      541 aatatgggca ccgtgaaggt gtgtctcggc ggcaatggag cggttgtgct actcggagtg
      601 ctcaactggc caccggcagc agccaccgca ctcagtttgg ccagcagtgg gctggagttg
      661 gagttaccgt tgtgcagctc cgccttgatg tccttgatgt cgctgaagtt gaacgagggc
      721 gagggcttgt ccagccggtt ggcggaactc gctcccgcgt tgctggactc ggtgtgaacc
      781 tctaggggta tttctccatt ggccgctgcc tcggccaact tccgcttctt ggccataatt
      841 ggtgctatct cgggttcagt gccgtcct
//
