LOCUS       sequence-414743-       11751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pMVP Gateway destination vector (3rd generation lentiviral vector) 
            containing human full length PRB gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11751)
  AUTHORS   Aarts MT, Wagner M, van der Wal T, van Boxtel AL, van Amerongen R
  TITLE     A molecular toolbox to study progesterone receptor signaling.
  JOURNAL   J Mammary Gland Biol Neoplasia. 2023 Nov 29;28(1):24. doi: 
            10.1007/s10911-023-09550-0.
  PUBMED    38019315
REFERENCE   2  (bases 1 to 11751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mammary 
            Gland Biol Neoplasia."; date: "2023-11-29"; volume: "28(1):24. doi";
            pages: " 10.1007/s10911-023-09550-0"
COMMENT     Sequence Label: Lenti-CMV-PRB
FEATURES             Location/Qualifiers
     source          1..11751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1981..2005
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        2161..2540
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2541..2744
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2695..2715
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2741..2765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(2773..2793)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     RBS             6582..6593
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    6663..6683
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6860..6879)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     protein_bind    complement(7281..7305)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7357..7673
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7524..7659
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7721..7768
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7787..8185
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             8272..8505
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8577..8698
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8614..8633)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8668..8687
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8738..8873
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8893..8912)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8894..8912)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8922..8939)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8922..8938)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8931..8953)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9080..9535
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9167..9186)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9377..9398
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9561..9665
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9666..9734,9735..10526)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9884..9903)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10697..11285
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11186..11205
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11439..11456
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11573..11594
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11609..11614,11615..11632,11633..11639)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11647..11663
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11652..11674
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11671..11687
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11671..11687
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11706..11726
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11708..11726
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgtctaga ggatccacta gtccagtgtg gtggaattct gcagatatca
     1981 acaagtttgt acaaaaaagc aggcttacga tctatacatt gaatcaatat tggcaattag
     2041 ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
     2101 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgccatgtt
     2161 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     2221 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     2281 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     2341 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     2401 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     2461 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
     2521 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
     2581 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     2641 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
     2701 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
     2761 agatccggca cccaactttt ctatacaaag ttgggagacc caagcttggt accatgactg
     2821 agctgaaggc aaagggtccc cgggctcccc acgtggcggg cggcccgccc tcccccgagg
     2881 tcggatcccc actgctgtgt cgcccagccg caggtccgtt cccggggagc cagacctcgg
     2941 acaccttgcc tgaagtttcg gccataccta tctccctgga cgggctactc ttccctcggc
     3001 cctgccaggg acaggacccc tccgacgaaa agacgcagga ccagcagtcg ctgtcggacg
     3061 tggagggcgc atattccaga gctgaagcta caaggggtgc tggaggcagc agttctagtc
     3121 ccccagaaaa ggacagcgga ctgctggaca gtgtcttgga cactctgttg gcgccctcag
     3181 gtcccgggca gagccaaccc agccctcccg cctgcgaggt caccagctct tggtgcctgt
     3241 ttggccccga acttcccgaa gatccaccgg ctgcccccgc cacccagcgg gtgttgtccc
     3301 cgctcatgag ccggtccggg tgcaaggttg gagacagctc cgggacggca gctgcccata
     3361 aagtgctgcc ccggggcctg tcaccagccc ggcagctgct gctcccggcc tctgagagcc
     3421 ctcactggtc cggggcccca gtgaagccgt ctccgcaggc cgctgcggtg gaggttgagg
     3481 aggaggatgg ctctgagtcc gaggagtctg cgggtccgct tctgaagggc aaacctcggg
     3541 ctctgggtgg cgcggcggct ggaggaggag ccgcggctgt cccgccgggg gcggcagcag
     3601 gaggcgtcgc cctggtcccc aaggaagatt cccgcttctc agcgcccagg gtcgccctgg
     3661 tggagcagga cgcgccgatg gcgcccgggc gctccccgct ggccaccacg gtgatggatt
     3721 tcatccacgt gcctatcctg cctctcaatc acgccttatt ggcagcccgc actcggcagc
     3781 tgctggaaga cgaaagttac gacggcgggg ccggggctgc cagcgccttt gccccgccgc
     3841 ggagttcacc ctgtgcctcg tccaccccgg tcgctgtagg cgacttcccc gactgcgcgt
     3901 acccgcccga cgccgagccc aaggacgacg cgtaccctct ctatagcgac ttccagccgc
     3961 ccgctctaaa gataaaggag gaggaggaag gtgcggaggc ctccgcgcgc tccccgcgtt
     4021 cctaccttgt ggccggtgcc aaccccgcag ccttcccgga tttcccgttg gggccaccgc
     4081 ccccgctgcc gccgcgagcg accccatcca gacccgggga agcggcggtg acggccgcac
     4141 ccgccagtgc ctcagtctcg tctgcgtcct cctcggggtc gaccctggag tgcatcctgt
     4201 acaaagcgga gggcgcgccg ccccagcagg gcccgttcgc gccgccgccc tgcaaggcgc
     4261 cgggcgcgag cggctgcctg ctcccgcggg acggcctgcc ctccacctcc gcctctgccg
     4321 ccgccgccgg ggcggccccc gcgctctacc ctgcactcgg cctcaacggg ctcccgcagc
     4381 tcggctacca ggccgccgtg ctcaaggagg gcctgccgca ggtctacccg ccctatctca
     4441 actacctgag gccggattca gaagccagcc agagcccaca atacagcttc gagtcattac
     4501 ctcagaagat ttgtttaatc tgtggggatg aagcatcagg ctgtcattat ggtgtcctta
     4561 cctgtgggag ctgtaaggtc ttctttaaga gggcaatgga agggcagcac aactacttat
     4621 gtgctggaag aaatgactgc atcgttgata aaatccgcag aaaaaactgc ccagcatgtc
     4681 gccttagaaa gtgctgtcag gctggcatgg tccttggagg tcgaaaattt aaaaagttca
     4741 ataaagtcag agttgtgaga gcactggatg ctgttgctct cccacagcca ttgggcgttc
     4801 caaatgaaag ccaagcccta agccagagat tcactttttc accaggtcaa gacatacagt
     4861 tgattccacc actgatcaac ctgttaatga gcattgaacc agatgtgatc tatgcaggac
     4921 atgacaacac aaaacctgac acctccagtt ctttgctgac aagtcttaat caactaggcg
     4981 agaggcaact tctttcagta gtcaagtggt ctaaatcatt gccaggtttt cgaaacttac
     5041 atattgatga ccagataact ctcattcagt attcttggat gagcttaatg gtgtttggtc
     5101 taggatggag atcctacaaa catgtcagtg ggcagatgct gtattttgca cctgatctaa
     5161 tactaaatga acagcggatg aaagaatcat cattctattc attatgcctt accatgtggc
     5221 agatcccaca ggagtttgtc aagcttcaag ttagccaaga agagttcctc tgtatgaaag
     5281 tattgttact tcttaataca attcctttgg aagggctacg aagtcaaacc cagtttgagg
     5341 agatgaggtc aagctacatt agagagctca tcaaggcaat tggtttgagg caaaaaggag
     5401 ttgtgtcgag ctcacagcgt ttctatcaac ttacaaaact tcttgataac ttgcatgatc
     5461 ttgtcaaaca gcttcatctg tactgcttga atacatttat ccagtcccgg gcactgagtg
     5521 ttgaatttcc agaaatgatg tctgaagtta ttgctgcaca attacccaag atattggcag
     5581 ggatggtgaa accccttctc tttcataaaa agtgaatgtc atctttttct tttaaagaat
     5641 taaattttgt ggcatgtctt tttgttttgg tcaggattat gaggtcttga gtttttataa
     5701 tgttcttctg aaagccttac atttataaca tcatagtgtg taaatttaaa agaaaaattg
     5761 tgaggttcta attattttct tttataaagt ataattagaa tgtttaactg ttttgtttac
     5821 ccatattttc ttgaagaatt tacaagattg aaaaagtact aaaattgtta aagtaaacta
     5881 tatcttatcc atattatttc ataccatgta ggtgaggatt tttaactttt gcatctaaca
     5941 aatcatcgac ttaagagaaa aaatcttaca tgtaataaca caaagctatt atatgttatt
     6001 tctaggtaac tccctttgtg tcaattatat ttccaaaaat gaacctttaa aatggtatgc
     6061 aaaattttgt ctatatatat ttgtgtgagg aggaaattca taactttcct cagattttca
     6121 aaagtatttt taatgcaaaa aatgtagaaa gagtttaaaa ccactaaaat agattgatgt
     6181 tcttcaaact aggcaaaaca actcatatgt taagaccatt ttccagattg gaaacacaaa
     6241 tctcttagga agttaataag tagattcata tcattataca aatagtattg tgggttttgt
     6301 aggtttttaa aataaccttt tttggggaga gaattgtcct ctaatgaggt attgcgagtg
     6361 gacataagaa atcagaagat tatggcctaa ctgtactcct taccaactgt ggcatgctga
     6421 aagttagtca ctcttactga ttctcaattc tctcaccttt gaaagtagta aaatatcttt
     6481 cctgccaatt gctcctttgg gtcagagctt attaacatct tttcaaatca aaggaaagaa
     6541 gaaagggaga ggaggaggag ggagatatca attcacatac ctttctcctc tttatcctcc
     6601 actatcatga attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagag
     6661 gacaactttg tataataaag ttgtacagtg cctctcctgg ccttggaagt tgccactcca
     6721 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
     6781 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
     6841 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
     6901 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
     6961 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
     7021 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
     7081 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg
     7141 attttaaaat aactatacca gcaggaggac gtccagacac agcataggct acctggccat
     7201 gcccaaccgg tgggacattt gagttgcttg cttggcactg tcctctcatg cgttgggtcc
     7261 actcagtaga tgcctgttga acccagcttt cttgtacaaa gtggttgata tccagcacag
     7321 tggcggccgc tcgagtctag aggtggcgat ggttccggtg tggaaagtcc ccaggctccc
     7381 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     7441 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     7501 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     7561 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     7621 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     7681 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     7741 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagccttt
     7801 gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat
     7861 ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg
     7921 tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc
     7981 tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg
     8041 catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa
     8101 agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc
     8161 ctctggttat gtgtgggagg gctaagcaca attcgagctc ggtaccttta agaccaatga
     8221 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     8281 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     8341 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8401 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8461 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     8521 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     8581 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     8641 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     8701 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     8761 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     8821 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     8881 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     8941 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     9001 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     9061 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     9121 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     9181 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     9241 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     9301 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     9361 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     9421 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     9481 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     9541 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     9601 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     9661 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     9721 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     9781 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     9841 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     9901 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     9961 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    10021 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    10081 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    10141 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
    10201 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    10261 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    10321 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    10381 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    10441 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    10501 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    10561 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    10621 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    10681 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    10741 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    10801 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    10861 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    10921 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    10981 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    11041 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    11101 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    11161 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    11221 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    11281 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    11341 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    11401 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    11461 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    11521 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    11581 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    11641 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
    11701 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct t
//
