LOCUS       sequence-41475-e         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Sato S, Tomomori-Sato C, Banks CA, Parmely TJ, Sorokina I, Brower 
            CS, Conaway RC, Conaway JW
  TITLE     A mammalian homolog of Drosophila melanogaster transcriptional 
            coactivator intersex is a subunit of the mammalian Mediator complex.
  JOURNAL   J Biol Chem. 2003 Dec 12. 278(50):49671-4.
  PUBMED    14576168
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Dec 12. 278(50):49671-4."
COMMENT     Sequence Label: FLAG-MED6
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..119
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagca tgccagacta caaggacgat gatgacaagc
      121 tcgagatggc ggcggtggat atccgagata acctgctggg gatctcctgg gttgacagct
      181 cttggattcc catcttgaac agtggcagtg tcctggatta cttttcagaa agaagcaatc
      241 ctttctatga caggacgtgt aataatgagg tggtcaaaat gcagaggctg acattagagc
      301 acttgaatca gatggttgga atcgaataca tccttttaca tgctcaagag cccattcttt
      361 tcatcattcg aaagcaacag cggcagtccc ctgctcaagt tattccactg gctgattact
      421 atatcatcgc tggagtcatc tatcaggcgc cagacttggg atcagttata aactccagag
      481 tgcttactgc agtgcatgga attcagtcag cttttgatga agctatgtca tactgtcgat
      541 accatccttc caaaggatat tggtggcact ttaaagatca cgaagaacaa gagaaagtca
      601 aacctaaagc caaaaggaaa gaagaaccaa gctctatttt tcaaagacag cgtgtggatg
      661 ctttactcat agaccttaga cagaaatttc cacccaga
//
