LOCUS       sequence-41476-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Kadener S, Menet JS, Schoer R, Rosbash M
  TITLE     Circadian transcription contributes to core period determination in 
            Drosophila.
  JOURNAL   PLoS Biol. 2008 May 20;6(5):e119.
  PUBMED    18494558
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2008-05-20"; volume: "6(5)"; pages: "e119"
COMMENT     Sequence Label: pAC-Cyc
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..53
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(76..93)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(103..144)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcctcgatc gaggctgatc agcgagctca ggccttagaa ggcacagtcg aggctgatca
       61 gcgggtttaa actcaatggt gatggtgatg atgaccggta cgcgtagaat cgagaccgag
      121 gagagggtta gggataggct taccttcgaa ccgcgggccc tctagactcg agcggccgca
      181 taagaacacg gaattcttgg cgattatata gtcaatctcg ctcgtccagg gattcttgaa
      241 ggcccgccac tcgctctgca gctgaatgta gctgttgtcc ttgcaccgga agcggtagac
      301 ctgagtggtc accttttccg gcacctgcat caccatcttg tgagactcca tcagcgcagc
      361 gatgtcttcg ttgtggaagt actcgtagaa gctggtgccg aggatctcct gaggcaggaa
      421 accaatcacg agggtggcac gctggtctat gaacaggaac ttgccctcgc cggagtggcg
      481 cgagatgaag agcacgtgcc gtatgttcgg gtgattgtcc agcgaggcgg gtactgtgga
      541 gttgcgaacg ttgggcggaa tgcgaccaat tgccacaagg caggatagat tcgttgtctg
      601 ctcgtcgctg tcggcgtcct ggtcctcgtc cttgatgggt gtccaggact tgaggtagcc
      661 cgtgcactgg ataacccggt acttgtggcc cgtactcagt ctggacttgc gcttcgtgga
      721 gctccgggag ctggaggacg tatcggactc ctccttgatc tggttgttgc tggcagtgcg
      781 cagcttcatg cggcagaaga aggagcgccg ggcacccgga cacaggcggc
//
