LOCUS       sequence-414794-        6711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of chicken ovalbumin as model antigen for study of MHC 
            class I and class II presentation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Diebold SS, Cotten M, Koch N, Zenke M
  TITLE     MHC class II presentation of endogenously expressed antigens by 
            transfected dendritic cells.
  JOURNAL   Gene Ther. 2001 Mar;8(6):487-93.
  PUBMED    11313828
REFERENCE   2  (bases 1 to 6711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 
            Ther."; date: "2001-03"; volume: "8(6)"; pages: "487-93"
COMMENT     Sequence Label: pcDNA3-OVA
FEATURES             Location/Qualifiers
     source          1..6711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(956..974)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(957..974)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(994..1011)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1000..1224
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1270..1698
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1357..1376)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1567..1588
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1707..1727)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1712..2041
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1892..2027
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1954..1973
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2108..2902
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2162..2181)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2772..2791
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3076..3197
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3113..3132)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3167..3186
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3246..3262)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3246..3262)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3259..3281)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3270..3286
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3294..3300,3301..3318,3319..3324))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3339..3360
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3477..3494)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3648..4236)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3728..3747)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4407..5198,5199..5267))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5030..5049
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5268..5372)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5447..5466)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5638..6017
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6018..6221
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6172..6192
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6266..6285
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6266..6284
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(6372..6711,1..821)
                     /codon_start=1
                     /product="chicken ovalbumin"
                     /label=ovalbumin
                     /translation="MGSIGAASMEFCFDVFKELKVHHANENIFYCPIAIMSALAMVYLG
                     AKDSTRTQINKVVRFDKLPGFGDSIEAQCGTSVNVHSSLRDILNQITKPNDVYSFSLAS
                     RLYAEERYPILPEYLQCVKELYRGGLEPINFQTAADQARELINSWVESQTNGIIRNVLQ
                     PSSVDSQTAMVLVNAIVFKGLWEKAFKDEDTQAMPFRVTEQESKPVQMMYQIGLFRVAS
                     MASEKMKILELPFASGTMSMLVLLPDEVSGLEQLESIINFEKLTEWTSSNVMEERKIKV
                     YLPRMKMEEKYNLTSVLMAMGITDVFSSSANLSGISSAESLKISQAVHAAHAEINEAGR
                     EVVGSAEAGVDAASVSEEFRADHPFLFCIKHIATNAVLFFGRCVSP"
ORIGIN
        1 tcctgccaga atacttgcag tgtgtgaagg aactgtatag aggaggcttg gaacctatca
       61 actttcaaac agctgcagat caagccagag agctcatcaa ttcctgggta gaaagtcaga
      121 caaatggaat tatcagaaat gtccttcagc caagctccgt ggattctcaa actgcaatgg
      181 ttctggttaa tgccattgtc ttcaaaggac tgtgggagaa agcatttaag gatgaagaca
      241 cacaagcaat gcctttcaga gtgactgagc aagaaagcaa acctgtgcag atgatgtacc
      301 agattggttt atttagagtg gcatcaatgg cttctgagaa aatgaagatc ctggagcttc
      361 catttgccag tgggacaatg agcatgttgg tgctgttgcc tgatgaagtc tcaggccttg
      421 agcagcttga gagtataatc aactttgaaa aactgactga atggaccagt tctaatgtta
      481 tggaagagag gaagatcaaa gtgtacttac ctcgcatgaa gatggaggaa aaatacaacc
      541 tcacatctgt cttaatggct atgggcatta ctgacgtgtt tagctcttca gccaatctgt
      601 ctggcatctc ctcagcagag agcctgaaga tatctcaagc tgtccatgca gcacatgcag
      661 aaatcaatga agcaggcaga gaggtggtag ggtcagcaga ggctggagtg gatgctgcaa
      721 gcgtctctga agaatttagg gctgaccatc cattcctctt ctgtatcaag cacatcgcaa
      781 ccaacgccgt tctcttcttt ggcagatgtg tttcccctta aaaagaagaa agctgaaaaa
      841 ctctgtccct tccaacaaga cccagagcac tgtagtatca ggggtaaaat gaaaagtatg
      901 ttatctgctg catccagact tcataaaagc tggagcttaa tctagagggc cctattctat
      961 agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     1021 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     1081 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     1141 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     1201 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     1261 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     1321 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     1381 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     1441 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     1501 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     1561 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     1621 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     1681 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     1741 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     1801 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     1861 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     1921 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     1981 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     2041 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
     2101 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     2161 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     2221 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     2281 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     2341 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     2401 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     2461 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     2521 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     2581 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     2641 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     2701 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     2761 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     2821 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     2881 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     2941 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     3001 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     3061 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     3121 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     3181 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     3241 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     3301 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     3361 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     3421 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     3481 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     3541 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     3601 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     3661 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     3721 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3781 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3841 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3901 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3961 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4021 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4081 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4141 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4201 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4261 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     4321 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     4381 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     4441 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     4501 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     4561 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     4621 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     4681 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     4741 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     4801 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     4861 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     4921 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     4981 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     5041 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     5101 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     5161 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     5221 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     5281 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     5341 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     5401 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     5461 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     5521 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     5581 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
     5641 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     5701 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     5761 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     5821 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     5881 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     5941 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     6001 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     6061 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     6121 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     6181 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     6241 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagcttggt
     6301 accgagctcg gatccactag taacggccgc cagtgtgctg gaattcctcg aaagacaact
     6361 cagagttcac catgggctcc atcggtgcag caagcatgga attttgtttt gatgtattca
     6421 aggagctcaa agtccaccat gccaatgaga acatcttcta ctgccccatt gccatcatgt
     6481 cagctctagc catggtatac ctgggtgcaa aagacagcac caggacacaa ataaataagg
     6541 ttgttcgctt tgataaactt ccaggattcg gagacagtat tgaagctcag tgtggcacat
     6601 ctgtaaacgt tcactcttca cttagagaca tcctcaacca aatcaccaaa ccaaatgatg
     6661 tttattcgtt cagccttgcc agtagacttt atgctgaaga gagataccca a
//
