LOCUS       sequence-414797-        8815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for inducible knockdown of human ARNT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Halawani D, Wang Y, Ramakrishnan A, Estill M, He X, Shen L, Friedel 
            RH, Zou H
  TITLE     Circadian clock regulator Bmal1 gates axon regeneration via Tet3 
            epigenetics in mouse sensory neurons.
  JOURNAL   Nat Commun. 2023 Aug 24;14(1):5165. doi: 10.1038/s41467-023-40816-7.
  PUBMED    37620297
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-24"; volume: "14(1):5165. doi"; pages: " 
            10.1038/s41467-023-40816-7"
COMMENT     Sequence Label: pLKO-Tet-On-ARNT-shRNA2
FEATURES             Location/Qualifiers
     source          1..8815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     286..306
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             517..750
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    822..943
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(859..878)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     913..932
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      983..1118
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(1139..1157)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1167..1184)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1167..1183)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1176..1198)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1325..1780
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1412..1431)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1622..1643
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1806..1910
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1911..1979,1980..2771)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2129..2148)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2942..3530
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3431..3450
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3684..3701
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3818..3839
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3854..3859,3860..3877,3878..3884)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3892..3908
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3897..3919
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3916..3932
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3916..3932
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3951..3971
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3953..3971
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3999..4225
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4226..4406
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4453..4578
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5071..5304
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5489..5533
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5822..5916
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     5833..5852
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    5870..5888
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5898..5916
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    6016..6133
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6182..6692
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6611..6629
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          6701..7273
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     7281..7300
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        7328..7346
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7357..7977
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(7430..7449)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    8012..8585
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8179..8196)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8406..8425
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(8605..8815,1..389)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8605..8624)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
       61 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
      121 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
      181 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
      241 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
      301 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
      361 ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg
      421 aaaggagcgc acgaccccat gcatcggtac ctttaagacc aatgacttac aaggcagctg
      481 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
      541 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
      601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
      661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
      721 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
      781 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
      841 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
      901 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
      961 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     1021 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     1081 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     1141 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     1201 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     1261 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     1321 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     1381 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     1441 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     1501 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     1561 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     1621 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     1681 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     1741 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     1801 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1861 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     1921 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     1981 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     2041 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     2101 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     2161 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     2221 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     2281 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     2341 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     2401 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     2461 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     2521 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     2581 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     2641 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     2701 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     2761 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     2821 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     2881 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2941 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3001 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3061 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3121 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3181 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3241 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3301 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3361 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3421 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3481 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3541 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3601 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3661 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     3721 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     3781 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     3841 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     3901 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     3961 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     4021 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     4081 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     4141 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     4201 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     4261 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     4321 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     4381 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     4441 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     4501 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     4561 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     4621 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     4681 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     4741 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     4801 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     4861 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     4921 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     4981 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     5041 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     5101 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     5161 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     5221 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     5281 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     5341 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     5401 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     5461 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     5521 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     5581 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     5641 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     5701 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     5761 cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagcctc gagcggccgc
     5821 aatatttgca tgtcgctatg tgttctggga aatcaccata aacgtgaaat ccctatcagt
     5881 gatagagact tataagttcc ctatcagtga tagagacacc gggcctacac tctccaacac
     5941 aatctcgaga ttgtgttgga gagtgtaggc tttttaattc tcgacctcga gacaaatggc
     6001 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     6061 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     6121 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc cgcggctcga
     6181 gggggttggg gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct
     6241 ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt
     6301 ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc
     6361 ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa
     6421 cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag
     6481 cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca
     6541 gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc
     6601 tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc
     6661 tcgttgaccg aatcaccgac ctctctcccc agggggatct gtgagtttgg ggacccttga
     6721 ttgttctttc tttttcgcta ttgtaaaatt catgttatat ggagggggca aagttttcag
     6781 ggtgttgttt agaatgggaa gatgtccctt gtatcaccat ggaccctcat gataattttg
     6841 tttctttcac tttctactct gttgacaacc attgtctcct cttattttct tttcattttc
     6901 tgtaactttt tcgttaaact ttagcttgca tttgtaacga atttttaaat tcacttttgt
     6961 ttatttgtca gattgtaagt actttctcta atcacttttt tttcaaggca atcagggtat
     7021 attatattgt acttcagcac agttttagag aacaattgtt ataattaaat gataaggtag
     7081 aatatttctg catataaatt ctggctggcg tggaaatatt cttattggta gaaacaacta
     7141 catcctggtc atcatcctgc ctttctcttt atggttacaa tgatatacac tgtttgagat
     7201 gaggataaaa tactctgagt ccaaaccggg cccctctgct aaccatgttc atgccttctt
     7261 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
     7321 agaattgtaa tacgactcac tatagggcga attgatatgt ctagattaga taaaagtaaa
     7381 gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt
     7441 aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag
     7501 cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc
     7561 cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt
     7621 gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa
     7681 cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag
     7741 aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat
     7801 caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca
     7861 ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc
     7921 ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgcg
     7981 tacagcggct cccgggagtt ctagggatct gcccctctcc ctcccccccc cctaacgtta
     8041 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     8101 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     8161 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     8221 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     8281 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     8341 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     8401 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     8461 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     8521 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     8581 gataaggatc caccggagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
     8641 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     8701 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     8761 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcg
//
