LOCUS       sequence-414810-       12416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PIEZO1 fusion for biochemistry and live imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12416)
  AUTHORS   Zhou Z, Ma X, Lin Y, Cheng D, Bavi N, Secker GA, Li JV, Janbandhu V,
            Sutton DL, Scott HS, Yao M, Harvey RP, Harvey NL, Corry B, Zhang Y, 
            Cox CD
  TITLE     MyoD-family inhibitor proteins act as auxiliary subunits of Piezo 
            channels.
  JOURNAL   Science. 2023 Aug 18;381(6659):799-804. doi: 
            10.1126/science.adh8190. Epub 2023 Aug 17.
  PUBMED    37590348
REFERENCE   2  (bases 1 to 12416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adh8190"; journalName: "Science"; date: 
            "2023-08-18- 18"; volume: "381"; issue: "6659"; pages: "799-804"
COMMENT     Sequence Label: PIEZO1-HaloTag
FEATURES             Location/Qualifiers
     source          1..12416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             8185..9075
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     polyA_signal    9204..9325
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9241..9260)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9295..9314
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9332..9787)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9469..9490)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9681..9700
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9814..9918
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9920..10277
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9920..10110
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9943..9963)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10128..10263
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10312..11106
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10312..11106
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10366..10385)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10976..10995
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11294..11313)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11338..11385
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11714..12302
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12203..12222
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcct
      601 cagcaagggc tgaggatgga gccgcacgtg ttaggcgcgg tcctgtactg gctgctgctg
      661 ccctgcgcgc tgctggctgc ctgcctgctc cgcttcagcg gactctcgct ggtctacctg
      721 ctcttcctgc tgctgctgcc ctggttcccc ggccccaccc gatgcggcct ccaaggtcac
      781 acaggccgcc tcctgcgggc attgctgggc ctcagcctgc tcttcctggt ggcccatctc
      841 gccctccaga tctgcctgca tattgtgccc cgcctggacc agctcctggg acccagctgc
      901 agccgctggg agaccctctc gcgacacata ggggtcacaa ggctggacct gaaggacatc
      961 cccaacgcca tccggctggt ggcccctgac ctgggcatct tggtggtctc ctctgtctgc
     1021 ctcggcatct gcgggcgcct tgcaaggaac acccggcaga gcccacatcc acgggagctg
     1081 gatgatgatg agagggatgt ggatgccagc ccgacggcag ggctgcagga agcagcaacg
     1141 ctggccccta cacggaggtc acggctggcc gctcgtttcc gagtcacggc ccactggctg
     1201 ctggtggcgg ctgggcgggt cctggccgta acactgcttg cactggcagg catcgcccac
     1261 ccctcggccc tctccagtgt ctacctgctg ctcttcctgg ccctctgcac ctggtgggcc
     1321 tgccactttc ccatcagcac tcggggcttc agcagactct gcgtcgcggt ggggtgcttc
     1381 ggcgccggcc atctcatctg cctctactgc taccagatgc ccttggcaca ggctctgctc
     1441 ccgcctgccg gcatctgggc tagggtgctg ggtctcaagg acttcgtggg tcccaccaac
     1501 tgctccagcc cccacgcgct ggtcctcaac accggcctgg actggcctgt gtatgccagc
     1561 cccggcgtcc tcctgctgct gtgctacgcc acggcctctc tgcgcaagct ccgcgcgtac
     1621 cgcccctccg gccagaggaa ggaggcggca aaggggtatg aggctcggga gctggagcta
     1681 gcagagctgg accagtggcc ccaggaacgg gagtctgacc agcacgtggt gcccacagca
     1741 cccgacaccg aggctgataa ctgcatcgtg cacgagctga ccggccagag ctccgtcctg
     1801 cggcggcctg tgcggcccaa gcgggctgag cccagggagg cgtctccgct ccacagcctg
     1861 ggccacctca tcatggacca gagctatgtg tgcgcgctca ttgccatgat ggtatggagc
     1921 atcacctacc acagctggct gaccttcgta ctgctgctct gggcctgcct catctggacg
     1981 gtgcgcagcc gccaccaact ggccatgctg tgctcgccct gcatcctgct gtatgggatg
     2041 acgctgtgct gcctacgcta cgtgtgggcc atggacctgc gccctgagct gcccaccacc
     2101 ctgggccccg tcagcctgcg ccagctgggg ctggagcaca cccgctaccc ctgtctggac
     2161 cttggtgcca tgttgctcta caccctgacc ttctggctcc tgctgcgcca gtttgtgaaa
     2221 gagaagctgc tgaagtgggc agagtctcca gctgcgctga cggaggtcac cgtggcagac
     2281 acagagccca cgcggacgca gacgctgttg cagagcctgg gggagctggt gaagggcgtg
     2341 tacgccaagt actggatcta tgtgtgtgct ggcatgttca tcgtggtcag cttcgccggc
     2401 cgcctcgtgg tctacaagat tgtctacatg ttcctcttcc tgctctgcct caccctcttc
     2461 caggtctact acagcctgtg gcggaagctg ctcaaggcct tctggtggct cgtggtggcc
     2521 tacaccatgc tggtcctcat cgccgtctac accttccagt tccaggactt ccctgcctac
     2581 tggcgcaacc tcactggctt caccgacgag cagctggggg acctgggcct ggagcagttc
     2641 agcgtgtccg agctcttctc cagcatcctg gtgcccggct tcttcctcct ggcctgcatc
     2701 ctgcagctgc actacttcca caggcccttc atgcagctca ccgacatgga gcacgtgtcc
     2761 ctgcctggca cgcgcctccc gcgctgggct cacaggcagg atgcagtgag tgggacccca
     2821 ctgctgcggg aggagcagca ggagcatcag cagcagcagc aggaggagga ggaggaggag
     2881 gaggactcca gggacgaggg gctgggcgtg gccactcccc accaggccac gcaggtgcct
     2941 gaaggggcag ccaagtgggg cctggtggct gagcggctgc tggagctggc agccggcttc
     3001 tcggacgtcc tctcacgcgt gcaggtgttc ctgcggcggc tgctggagct tcacgttttc
     3061 aagctggtgg ccctgtacac cgtctgggtg gccctgaagg aggtgtcggt gatgaacctg
     3121 ctgctggtgg tgctgtgggc cttcgccctg ccctacccac gcttccggcc catggcctcc
     3181 tgcctgtcca ccgtgtggac ctgcgtcatc atcgtgtgta agatgctgta ccagctcaag
     3241 gttgtcaacc cccaggagta ttccagcaac tgcaccgagc ccttccccaa cagcaccaac
     3301 ttgctgccca cggagatcag ccagtccctg ctgtaccggg ggcccgtgga ccctgccaac
     3361 tggtttgggg tgcggaaagg gttccccaac ctgggctaca tccagaacca cctgcaagtg
     3421 ctgctgctgc tggtattcga ggccatcgtg taccggcgcc aggagcacta ccgccggcag
     3481 caccagctgg ccccgctgcc tgcccaggcc gtgtttgcca gcggcacccg ccagcagctg
     3541 gaccaggatc tgctcggctg cctcaagtac ttcatcaact tcttcttcta caaattcggg
     3601 ctggagatct gcttcctgat ggccgtgaac gtgatcgggc agcgcatgaa ctttctggtg
     3661 accctgcacg gttgctggct ggtggccatc ctcacccgca ggcaccgcca ggccattgcc
     3721 cgcctctggc ccaactactg cctcttcctg gcgctgttcc tgctgtacca gtacctgctg
     3781 tgcctgggga tgcccccggc cctgtgcatt gattatccct ggcgctggag ccgggccgtc
     3841 cccatgaact ccgcactcat caagtggctg tacctgcctg atttcttccg ggcccccaac
     3901 tccaccaacc tcatcagcga ctttctcctg ctgctgtgcg cctcccagca gtggcaggtg
     3961 ttctcagctg agcgcacaga ggagtggcag cgcatggctg gcgtcaacac cgaccgcctg
     4021 gagccgctgc ggggggagcc caaccccgtg cccaacttta tccactgcag gtcctacctt
     4081 gacatgctga aggtggccgt cttccgatac ctgttctggc tggtgctggt ggtggtgttt
     4141 gtcacggggg ccacccgcat cagcatcttc gggctgggct acctgctggc ctgcttctac
     4201 ctgctgctct tcggcacggc cctgctgcag agggacacac gggcccgcct cgtgctgtgg
     4261 gactgcctca ttctgtacaa cgtcaccgtc atcatctcca agaacatgct gtcgctcctg
     4321 gcctgcgtct tcgtggagca gatgcagacc ggcttctgct gggtcatcca gctcttcagc
     4381 cttgtatgca ccgtcaaggg ctactatgac cccaaggaga tgatggacag agaccaggac
     4441 tgcctgctgc ctgtggagga ggctggcatc atctgggaca gcgtctgctt cttcttcctg
     4501 ctgctgcagc gccgcgtctt ccttagccat tactacctgc acgtcagggc cgacctccag
     4561 gccaccgccc tgctagcctc caggggcttc gccctctaca acgctgccaa cctcaagagc
     4621 attgacttcc accgcaggat agaggagaag tccctggccc agctgaaaag acagatggag
     4681 cgtatccgtg ccaagcagga gaagcacagg cagggccggg tggaccgcag tcgcccccag
     4741 gacaccctgg gccccaagga ccccggcctg gagccagggc ccgacagtcc agggggctcc
     4801 tccccgccac ggaggcagtg gtggcggccc tggctggacc acgccacagt catccactcc
     4861 ggggactact tcctgtttga gtccgacagt gaggaagagg aggaggctgt tcctgaagac
     4921 ccgaggccgt cggcacagag tgccttccag ctggcgtacc aggcatgggt gaccaacgcc
     4981 caggcggtgc tgaggcggcg gcagcaggag caggagcagg caaggcagga acaggcagga
     5041 cagctaccca caggaggtgg tcccagccag gaggtggagc cagcagaggg ccccgaggag
     5101 gcagcggcag gccggagcca tgtggtgcag agggtgctga gcacggcgca gttcctgtgg
     5161 atgctggggc aggcgctagt ggatgagctg acacgctggc tgcaggagtt cacccggcac
     5221 cacggcacca tgagcgacgt gctgcgggca gagcgctacc tcctcacaca ggagctcctg
     5281 cagggcggcg aagtgcacag gggcgtgctg gatcagctgt acacaagcca ggccgaggcc
     5341 acgttgccag gccccaccga ggcccccaat gccccaagca ccgtgtccag tgggctgggc
     5401 gcggaggagc cactcagcag catgacagac gacatgggca gccccctgag caccggctac
     5461 cacacgcgca gtggcagtga ggaggcagtc accgaccccg gggagcgtga ggctggtgcc
     5521 tctctgtacc agggactgat gcggacggcc agcgagctgc tcctggacag gcgcctgcgc
     5581 atcccagagc tggaggaggc agagctgttt gcggaggggc agggccgggc gctgcggctg
     5641 ctgcgggccg tgtaccagtg tgtggccgcc cactcggagc tgctctgcta cttcatcatc
     5701 atcctcaacc acatggtcac ggcctccgcc ggctcgctgg tgctgcccgt gctcgtcttc
     5761 ctgtgggcca tgctgtcgat cccgaggccc agcaagcgct tctggatgac ggccatcgtc
     5821 ttcaccgaga tcgcggtggt cgtcaagtac ctgttccagt ttgggttctt cccctggaac
     5881 agccacgtgg tgctgcggcg ctacgagaac aagccctact tcccgccccg catcctgggc
     5941 ctggagaaga ctgacggcta catcaagtac gacctggtgc agctcatggc ccttttcttc
     6001 caccgctccc agctgctgtg ctatggcctc tgggaccatg aggaggactc accatccaag
     6061 gagcatgaca agagcggcga ggaggagcag ggagccgagg aggggccagg ggtgcctgcg
     6121 gccaccaccg aagaccacat tcaggtggaa gcgagggtcg gacccacgga cgggacccca
     6181 gaaccccaag tggagctcag gccccgtgat acgaggcgca tcagtctacg ttttagaaga
     6241 aggaagaagg agggcccagc acggaaagga gcggcagcca tcgaagctga ggacagggag
     6301 gaagaagagg gggaggaaga gaaagaggcc cccacgggga gagagaagag gccaagccgc
     6361 tctggaggaa gagtaagggc ggccgggcgg cggctgcagg gcttctgcct gtccctggcc
     6421 cagggcacat atcggccgct acggcgcttc ttccacgaca tcctgcacac caagtaccgc
     6481 gcagccaccg acgtctatgc cctcatgttc ctggctgatg ttgtcgactt catcatcatc
     6541 atttttggct tctgggcctt tgggaagcac tcggcggcca cagacatcac gtcctcccta
     6601 tcagacgacc aggtacccga ggctttcctg gtcatgctgc tgatccagtt cagtaccatg
     6661 gtggttgacc gcgccctcta cctgcgcaag accgtgctgg gcaagctggc cttccaggtg
     6721 gcgctggtgc tggccatcca cctatggatg ttcttcatcc tgcccgccgt cactgagagg
     6781 atgttcaacc agaatgtggt ggcccagctc tggtacttcg tgaagtgcat ctacttcgcc
     6841 ctgtccgcct accagatccg ctgcggctac cccacccgca tcctcggcaa cttcctcacc
     6901 aagaagtaca atcatctcaa cctcttcctc ttccaggggt tccggctggt gccgttcctg
     6961 gtggagctgc gggcagtgat ggactgggtg tggacggaca ccacgctgtc cctgtccagc
     7021 tggatgtgtg tggaggacat ctatgccaac atcttcatca tcaaatgcag ccgagagaca
     7081 gagaagaaat acccgcagcc caaagggcag aagaagaaga agatcgtcaa gtacggcatg
     7141 ggtggcctca tcatcctctt cctcatcgcc atcatctggt tcccgctgct cttcatgtcg
     7201 ctggtgcgct ccgtggttgg ggttgtcaac cagcccatcg atgtcaccgt caccctgaag
     7261 ctgggcggct atgagccgct gttcaccatg agcgcccagc agccgtccat catccccttc
     7321 acggcccagg cctatgagga gctgtcccgg cagtttgacc cccagccgct ggccatgcag
     7381 ttcatcagcc agtacagccc tgaggacatc gtcacggcgc agattgaggg cagctccggg
     7441 gcgctgtggc gcatcagtcc ccccagccgt gcccagatga agcgggagct ctacaacggc
     7501 acggccgaca tcaccctgcg cttcacctgg aacttccaga gggacctggc gaagggaggc
     7561 actgtggagt atgccaacga gaagcacatg ctggccctgg cccccaacag cactgcacgg
     7621 cggcagctgg ccagcctgct cgagggcacc tcggaccagt ctgtggtcat ccccaatctc
     7681 ttccccaagt acatccgtgc ccccaacggg cccgaagcca accctgtgaa gcagctgcag
     7741 cccaatgagg aggccgacta cctcggcgtg cgtatccagc tgcggaggga gcagggtgcg
     7801 ggggccaccg gcttcctcga atggtgggtc atcgaactgc aggagtgccg gaccgactgc
     7861 aacctgctgc ccatggtcat tttcagtgac aaggtcagcc caccgagcct cggcttcctg
     7921 gctggctacg gcatcatggg gctgtacgtg tccatcgtgc tggtcatcgg caagttcgtg
     7981 cgcggattct tcagcgagat ctcgcactcc attatgttcg aggagctgcc gtgcgtggac
     8041 cgcatcctca agctctgcca ggacatcttc ctggtgcggg agactcggga gctggagctg
     8101 gaggaggagt tgtacgccaa gctcatcttc ctctaccgct caccggagac catgatcaag
     8161 tggactcgtg agaaggagac aaccatggca gaaatcggta ctggctttcc attcgacccc
     8221 cattatgtgg aagtcctggg cgagcgcatg cactacgtcg atgttggtcc gcgcgatggc
     8281 acccctgtgc tgttcctgca cggtaacccg acctcctcct acgtgtggcg caacatcatc
     8341 ccgcatgttg caccgaccca tcgctgcatt gctccagacc tgatcggtat gggcaaatcc
     8401 gacaaaccag acctgggtta tttcttcgac gaccacgtcc gcttcatgga tgccttcatc
     8461 gaagccctgg gtctggaaga ggtcgtcctg gtcattcacg actggggctc cgctctgggt
     8521 ttccactggg ccaagcgcaa tccagagcgc gtcaaaggta ttgcatttat ggagttcatc
     8581 cgccctatcc cgacctggga cgaatggcca gaatttgccc gcgagacctt ccaggccttc
     8641 cgcaccaccg acgtcggccg caagctgatc atcgatcaga acgtttttat cgagggtacg
     8701 ctgccgatgg gtgtcgtccg cccgctgact gaagtcgaga tggaccatta ccgcgagccg
     8761 ttcctgaatc ctgttgaccg cgagccactg tggcgcttcc caaacgagct gccaatcgcc
     8821 ggtgagccag cgaacatcgt cgcgctggtc gaagaataca tggactggct gcaccagtcc
     8881 cctgtcccga agctgctgtt ctggggcacc ccaggcgttc tgatcccacc ggccgaagcc
     8941 gctcgcctgg ccaaaagcct gcctaactgc aaggctgtgg acatcggccc gggtctgaat
     9001 ctgctgcaag aagacaaccc ggacctgatc ggcagcgaga tcgcgcgctg gctgtcgacg
     9061 cttgaaattt ccggctaatg aagcggccgc gactctagat cataatcagc cataccacat
     9121 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     9181 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     9241 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     9301 tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa
     9361 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     9421 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     9481 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     9541 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     9601 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     9661 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     9721 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     9781 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     9841 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     9901 tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     9961 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
    10021 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
    10081 tgcatctcaa ttagtcagca accatagtcc tgcccctaac tccgcccatc ccgcccctaa
    10141 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
    10201 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    10261 gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa
    10321 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
    10381 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
    10441 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
    10501 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
    10561 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
    10621 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
    10681 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
    10741 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
    10801 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
    10861 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
    10921 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
    10981 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
    11041 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
    11101 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
    11161 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
    11221 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta
    11281 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
    11341 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc
    11401 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
    11461 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
    11521 ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg
    11581 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    11641 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    11701 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    11761 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    11821 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    11881 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    11941 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    12001 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    12061 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    12121 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    12181 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    12241 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    12301 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    12361 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat
//
