LOCUS       sequence-41482-e        2788 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Song H, Payne S, Gray M, You L
  TITLE     Spatiotemporal modulation of biodiversity in a synthetic 
            chemical-mediated ecosystem.
  JOURNAL   Nat Chem Biol. 2009 Dec;5(12):929-35. Epub 2009 Nov 1.
  PUBMED    19915540
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol."; date: "2009-12"; volume: "5(12)"; pages: "929-35. Epub 2009 
            Nov 1"
COMMENT     Sequence Label: ptetmCherry_prey_plasmid-2
FEATURES             Location/Qualifiers
     source          1..2788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     35..53
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(74..92,93..98,99..115,116..121,122..147)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    74..92
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    99..117
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             157..168
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             175..885
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(320..338)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     588..607
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      954..1040
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1204..1792)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1284..1303)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1880..1974)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1995..2654)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2569..2588
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2655..2757)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
       61 tcttcacctc gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac
      121 tgagcacatc agcaggacgc actgaccgaa ttcattaaag aggagaaagg taccatggtg
      181 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
      241 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      301 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
      361 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
      421 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
      481 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
      541 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
      601 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
      661 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
      721 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
      781 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
      841 gagggccgcc actccaccgg cggcatggac gagctgtaca agtaaaagct tggatccctg
      901 caggcctcag ggcccgatcg atgcggccgc ttaattaatt aatctagagg catcaaataa
      961 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1021 ctctcctgag taggacaaat ccgccgccct agacctaggc gttcggctgc ggcgagcggt
     1081 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1141 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1201 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1261 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1321 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1381 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1441 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1501 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1561 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1621 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     1681 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1741 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1801 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1861 ggtcatgact agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
     1921 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
     1981 agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
     2041 agcattctgc cgacatggaa gccatcacag acggcatgat gaacctgaat cgccagcggc
     2101 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     2161 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
     2221 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     2281 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     2341 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     2401 catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg
     2461 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     2521 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     2581 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     2641 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     2701 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg
     2761 tctcattttc gccagatatc gacgtcta
//
