LOCUS       sequence-414825-        6191 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  molecular monitoring of atypical BCR::ABL1 fusion transcripts 
            (e6a2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Schafer V, White HE, Gerrard G, Mobius S, Saussele S, Franke GN, 
            Mahon FX, Talmaci R, Colomer D, Soverini S, Machova Polakova K, 
            Cross NCP, Hochhaus A, Ernst T
  TITLE     Assessment of individual molecular response in chronic myeloid 
            leukemia patients with atypical BCR-ABL1 fusion transcripts: 
            recommendations by the EUTOS cooperative network.
  JOURNAL   J Cancer Res Clin Oncol. 2021 Oct;147(10):3081-3089. doi: 
            10.1007/s00432-021-03569-8. Epub 2021 Mar 7.
  PUBMED    33677711
REFERENCE   2  (bases 1 to 6191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00432-021-03569-8"; journalName: "J Cancer Res Clin 
            Oncol"; date: "2021-10"; volume: "147"; issue: "10"; pages: 
            "3081-3089"
COMMENT     Sequence Label: pUC18_BCR_GUSB_e6a2
FEATURES             Location/Qualifiers
     source          1..6191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1931..1947)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1931..1947)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1944..1966)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1955..1971
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1979..1985,1986..2003,2004..2009))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2024..2045
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2162..2179)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2333..2921)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2413..2432)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3092..3883,3884..3952))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3715..3734
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3953..4057)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4125..4143
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4181..4203)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4303..4322
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4516..4538
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4530..4547
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4531..4547
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agcatgcatc tagatgcatg ctcgagcggc cgccagtgtg atggatatct gcagaattcg
       61 gcttgtaaac gggctgtttt ccaaacattg tgacttggct actgagtggg gatacctggt
      121 ttcattggca atcttccagt atctctctcg caaaaggaac gctgcacttt ttggttgtct
      181 ctgccgagtg aagatcccct ttttattccc cagcactctc gtcggtgact gttcagtcat
      241 gaaatcggca aaattccaaa tgagctctcc aaccacgtat tttctgcgtt tttgatccag
      301 acccagatgg tactgctcta gcagactttt ctggtactct tcagtgaaca tcagaggtgg
      361 atcctggtga aaccctgcaa tcgtttctgc tccatactcg ctctgaataa tgggcttctg
      421 atacttctta taccagttct caaactgggt ggccagctgc agctgaatca actccaggtg
      481 cccgtagtcg tgataccaag agtagtagct gttcaaacag atcacatcca catacggagc
      541 ccccttgtct gctgcatagt tagagttgct cacaaaggtc acaggccggg aggggtccaa
      601 ggatttggtg tgagcgatca ccatcttcaa gtagtagcca gcagattcta ggtgggacgc
      661 aggctcgttg gccacagacc acatcacgac cgcggggtgg ttcttgtccc tacgcaccac
      721 ttcttccatc acctgcatgt ggtgatgcag agaaacgttg ttgaagaact gcggcagcgc
      781 caggcccacg ccgggacact catcgatgac cacaatccca tagcggtcac acatctgcat
      841 cacttcctct gcataggggt agtggctggt acggaaaaag ccgaattcca gcacactggc
      901 ggccgttact agtggatccg agctcggtac cgagctcgaa ttcggcttgt ccactcagcc
      961 actggattta agcagagttc aaatctgtac tgcaccctgg aggtggattc ctttgggtat
     1021 tttgtgaata aagcaaagac gcgcgtctac agggacacag ctgagccaaa ctggaacgag
     1081 gaatttgaga tagagctgga gggctcccag accctgagga tactgtgcta tgaaaagtgt
     1141 tacaacaaga cgaagatccc caaggaggac ggcgagagca cggacagact catggggaag
     1201 ggccaggtcc agctggaccc gcaggccctg caggacagag actggcagcg caccgtcatc
     1261 gccatgaatg ggatcgaagt aaagctctcg gtcaagttca acagcaggga gttcagcttg
     1321 aagaggatgc cgtcccgaaa acagacaggg gtcttcggag tcaagattgc tgtggtcacc
     1381 aagagagaga ggtccaaggt gccctacatc gtgcgccagt gcgtggagga gatcgagcgc
     1441 cgaggcatgg aggaggtggg catctaccgc gtgtccggtg tggccacgga catccaggca
     1501 ctgaaggcag ccttcgacgt caataacaag gatgtgtcgg tgatgatgag cgagatggac
     1561 gtgaacgcca tcgcaggcac gctgaagctg tacttccgtg agctgcccga gcccctcttc
     1621 actgacgagt tctaccccaa cttcgcagag ggcatcgctc tttcagaccc ggttgcaaag
     1681 gagagctgca tgctcaacct gctgctgtcc ctgccggagg ccaacctgct caccttcctt
     1741 ttccttctgg accacctgaa aagggtggca gagaaggagg cagtcaataa gatgtccctg
     1801 cacaacctcg ccacggtctt tggccccacg ctgctccggc cctccgagaa ggagagcaag
     1861 ctccctgcca accccagcca gcctatcacc atgactgaca gctggtcctt gaagccgaat
     1921 tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     1981 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     2041 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     2101 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     2161 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2221 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2281 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2341 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2401 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2461 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2521 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2581 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2641 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2701 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2761 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2821 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2881 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2941 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3001 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3061 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3121 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3181 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     3241 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3301 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3361 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3421 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3481 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3541 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3601 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     3661 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3721 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3781 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3841 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3901 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3961 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4021 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4081 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     4141 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     4201 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     4261 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     4321 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     4381 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     4441 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     4501 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttggta
     4561 ccgagctcgg atccactagt aacggccgcc agtgtgctgg aattcgccct tcttcgtctg
     4621 agatactgga ttcctggaac attgtttcaa aggcttggtg gatttcagca aaggagggcc
     4681 ggtcagaggg attccactgc caaccttcta tcttgttctt tcttcacggt tatcaaagta
     4741 tgctcgcatg agttcataga ccttctctgg gcagccttct gggcgctcca tgcggtagtc
     4801 cttctctagc agctcataca cctgggacag gtcaattccc gggtaagggg acatgccata
     4861 ggtagcaatt tcccaaagca atactccaaa tgcccagacg tcggacttga tggagaactt
     4921 gttgtaggcc aggctctcgg gtgcagtcca tttgatgggg aacttggctc cagcatgggc
     4981 tgtgtaggtg tcccctgtca tcaacctgct caggccaaaa tcagctacct tcaccaagtg
     5041 gttctcccct accaggcagt ttcgggcagc aagatctctg tggatgaagt ttttcttctc
     5101 caggtactcc atggctgacg agatctgagt ggccatgtac agcagcacca cggcgttcac
     5161 ctcctgccgg ttgcactccc tcaggtagtc caggagattc ccgtaggtca tgaactcagt
     5221 gatgatatag aacgggggct cccgggtgca gaccccaagg agctgcacca ggttagggtg
     5281 tttgatctct ttcatgactg cagcttcttt caagaactct tccacctcca tggtgtcctc
     5341 cttcaaggtc ttcacggcca ccgtcaggct gtatttcttc cacacgccct cgtacacctc
     5401 cccgtactgg cccccgccca gcttgtgctt catggtgatg tccgtgcgtt ccatctccca
     5461 cttgtcgtag ttgggggaca caccatagac agtgggcttg ttgcgctttg gggctggata
     5521 atggagcgtg gtgatgagcc cgtcggccac cgttgaatga tgatgaacca actcggccag
     5581 ggtgttgaag cggctctcgg aggagacgta gagcttgcca tcagaagcag tgttgatcct
     5641 gtaatggtac accctccctt cgtatctcag cgagatggac ctctggccag gactgctctc
     5701 actctcacgc accaagaagc tgccattgat cccgctgctc agcagatact cagcggcatt
     5761 gcgggacaca ggcccatggt accaggagtg tttctccaga ctgttgactg gcgtgatgta
     5821 gttgcttggg acccagcctt ggccattttt ggtttgggct tcacaccatt ccccattgtg
     5881 attatagcct aagacccgga gcttttcacc tttagttatg cttagagtgt tatctccact
     5941 ggccacaaaa tcatacagtg caacgaaaag gttggggtca ttttcactgg gtccagcgag
     6001 aaggttttcc ttggagttcc aacgagcggc ttcactcaga ccctgaggct caaagtcaga
     6061 tgctactggc cgctgaaggg ctttttccag agagttcttg gtcgttggat ccttggcatc
     6121 tttgttgctt ctggctctca ggttctcgaa gggcgaattc tgcagatatc catcacactg
     6181 gcggccgctc g
//
