LOCUS       sequence-414826-        6165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  molecular monitoring of atypical BCR::ABL1 fusion transcripts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Schafer V, White HE, Gerrard G, Mobius S, Saussele S, Franke GN, 
            Mahon FX, Talmaci R, Colomer D, Soverini S, Machova Polakova K, 
            Cross NCP, Hochhaus A, Ernst T
  TITLE     Assessment of individual molecular response in chronic myeloid 
            leukemia patients with atypical BCR-ABL1 fusion transcripts: 
            recommendations by the EUTOS cooperative network.
  JOURNAL   J Cancer Res Clin Oncol. 2021 Oct;147(10):3081-3089. doi: 
            10.1007/s00432-021-03569-8. Epub 2021 Mar 7.
  PUBMED    33677711
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00432-021-03569-8"; journalName: "J Cancer Res Clin 
            Oncol"; date: "2021-10"; volume: "147"; issue: "10"; pages: 
            "3081-3089"
COMMENT     Sequence Label: pUC18_BCR_GUSB_e19a2
FEATURES             Location/Qualifiers
     source          1..6165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1925..1941)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1925..1941)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1938..1960)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1949..1965
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1973..1979,1980..1997,1998..2003))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2018..2039
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2156..2173)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2327..2915)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2407..2426)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3086..3877,3878..3946))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3709..3728
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3947..4051)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4119..4137
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4175..4197)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4297..4316
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4510..4532
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4524..4541
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4525..4541
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catctagatg catgctcgag cggccgccag tgtgatggat atctgcagaa ttcggcttgt
       61 aaacgggctg ttttccaaac attgtgactt ggctactgag tggggatacc tggtttcatt
      121 ggcaatcttc cagtatctct ctcgcaaaag gaacgctgca ctttttggtt gtctctgccg
      181 agtgaagatc ccctttttat tccccagcac tctcgtcggt gactgttcag tcatgaaatc
      241 ggcaaaattc caaatgagct ctccaaccac gtattttctg cgtttttgat ccagacccag
      301 atggtactgc tctagcagac ttttctggta ctcttcagtg aacatcagag gtggatcctg
      361 gtgaaaccct gcaatcgttt ctgctccata ctcgctctga ataatgggct tctgatactt
      421 cttataccag ttctcaaact gggtggccag ctgcagctga atcaactcca ggtgcccgta
      481 gtcgtgatac caagagtagt agctgttcaa acagatcaca tccacatacg gagccccctt
      541 gtctgctgca tagttagagt tgctcacaaa ggtcacaggc cgggaggggt ccaaggattt
      601 ggtgtgagcg atcaccatct tcaagtagta gccagcagat tctaggtggg acgcaggctc
      661 gttggccaca gaccacatca cgaccgcggg gtggttcttg tccctacgca ccacttcttc
      721 catcacctgc atgtggtgat gcagagaaac gttgttgaag aactgcggca gcgccaggcc
      781 cacgccggga cactcatcga tgaccacaat cccatagcgg tcacacatct gcatcacttc
      841 ctctgcatag gggtagtggc tggtacggaa aaagccgaat tccagcacac tggcggccgt
      901 tactagtgga tccgagctcg gtaccgagct cgaattcggc ttgtccactc agccactgga
      961 tttaagcaga gttcaaatct gtactgcacc ctggaggtgg attcctttgg gtattttgtg
     1021 aataaagcaa agacgcgcgt ctacagggac acagctgagc caaactggaa cgaggaattt
     1081 gagatagagc tggagggctc ccagaccctg aggatactgt gctatgaaaa gtgttacaac
     1141 aagacgaaga tccccaagga ggacggcgag agcacggaca gactcatggg gaagggccag
     1201 gtccagctgg acccgcaggc cctgcaggac agagactggc agcgcaccgt catcgccatg
     1261 aatgggatcg aagtaaagct ctcggtcaag ttcaacagca gggagttcag cttgaagagg
     1321 atgccgtccc gaaaacagac aggggtcttc ggagtcaaga ttgctgtggt caccaagaga
     1381 gagaggtcca aggtgcccta catcgtgcgc cagtgcgtgg aggagatcga gcgccgaggc
     1441 atggaggagg tgggcatcta ccgcgtgtcc ggtgtggcca cggacatcca ggcactgaag
     1501 gcagccttcg acgtcaataa caaggatgtg tcggtgatga tgagcgagat ggacgtgaac
     1561 gccatcgcag gcacgctgaa gctgtacttc cgtgagctgc ccgagcccct cttcactgac
     1621 gagttctacc ccaacttcgc agagggcatc gctctttcag acccggttgc aaaggagagc
     1681 tgcatgctca acctgctgct gtccctgccg gaggccaacc tgctcacctt ccttttcctt
     1741 ctggaccacc tgaaaagggt ggcagagaag gaggcagtca ataagatgtc cctgcacaac
     1801 ctcgccacgg tctttggccc cacgctgctc cggccctccg agaaggagag caagctccct
     1861 gccaacccca gccagcctat caccatgact gacagctggt ccttgaagcc gaattcgtaa
     1921 tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     1981 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     2041 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     2101 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     2161 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2221 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     2281 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2341 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2401 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2461 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2521 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2581 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2641 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2701 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2761 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2821 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2881 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2941 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     3001 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3061 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3121 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3181 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3241 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3301 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3361 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3421 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3481 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3541 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     3601 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     3661 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     3721 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     3781 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     3841 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     3901 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     3961 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4021 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     4081 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     4141 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     4201 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     4261 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     4321 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     4381 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     4441 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     4501 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt ggtaccgagc
     4561 tcggatccac tagtaacggc cgccagtgtg ctggaattcg cccttcttcg tctgagatac
     4621 tggattcctg gaacattgtt tcaaaggctt ggtggatttc agcaaaggag ggccggtcag
     4681 agggattcca ctgccaacat gctcgcatga gttcatagac cttctctggg cagccttctg
     4741 ggcgctccat gcggtagtcc ttctctagca gctcatacac ctgggacagg tcaattcccg
     4801 ggtaagggga catgccatag gtagcaattt cccaaagcaa tactccaaat gcccagacgt
     4861 cggacttgat ggagaacttg ttgtaggcca ggctctcggg tgcagtccat ttgatgggga
     4921 acttggctcc agcatgggct gtgtaggtgt cccctgtcat caacctgctc aggccaaaat
     4981 cagctacctt caccaagtgg ttctccccta ccaggcagtt tcgggcagca agatctctgt
     5041 ggatgaagtt tttcttctcc aggtactcca tggctgacga gatctgagtg gccatgtaca
     5101 gcagcaccac ggcgttcacc tcctgccggt tgcactccct caggtagtcc aggaggttcc
     5161 cgtaggtcat gaactcagtg atgacataga acgggggctc ccgggtgcag accccaagga
     5221 gctgcaccag gttagggtgt ttgatctctt tcatgactgc agcttctttc aagaactctt
     5281 ccacctccat ggtgtcctcc ttcaaggtct tcacggccac cgtcaggctg tatttcttcc
     5341 acgcgccctc gtacacctcc ccgtactggc ccccgcccag cttgtgcttc atggtgatgt
     5401 ccgtgcgttc catctcccac ttgtcgtagt tgggggacac accatagaca gtgggcttgt
     5461 tgcgctttgg ggctggataa tggagcgtgg tgatgagccc gtcggccacc gttgaatgat
     5521 gatgaaccaa ctcggccagg gtgttgaagc ggctctcgga ggagacgtag agcttgccat
     5581 cagaagcagt gttgatcctg taatggtaca ccctcccttc gtatctcagc gagatggacc
     5641 tctggccagg actgctctca ctctcacgca ccaagaagct gccattgatc ccgctgctca
     5701 gcagatactc agcggcattg cgggacacag gcccatggta ccaggagtgt ttctccagac
     5761 tgttgactgg cgtgatgtag ttgcttggga cccagccttg gccatttttg gtttgggctt
     5821 cacaccattc cccattgtga ttatagccta agacccggag cttttcacct ttagttatgc
     5881 ttagagtgtt atctccactg gccacaaaat catacagtgc aacgaaaagg ttggggtcat
     5941 tttcactggg tccagcgaga aggttttcct tggagttcca acgagcggct tcactcagac
     6001 cctgaggctc aaagtcagat gctactggcc gctgaagggc tttgacgtcg aaggctgcct
     6061 tcagtgcctg gatgtccgtg gccacaccgg acacgcggta gatgcccacc tcctccaagg
     6121 gcgaattctg cagatatcca tcacactggc ggccgctcga gcatg
//
