LOCUS       sequence-414831-        7776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  molecular monitoring of atypical BCR::ABL1 fusion transcripts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Schafer V, White HE, Gerrard G, Mobius S, Saussele S, Franke GN, 
            Mahon FX, Talmaci R, Colomer D, Soverini S, Machova Polakova K, 
            Cross NCP, Hochhaus A, Ernst T
  TITLE     Assessment of individual molecular response in chronic myeloid 
            leukemia patients with atypical BCR-ABL1 fusion transcripts: 
            recommendations by the EUTOS cooperative network.
  JOURNAL   J Cancer Res Clin Oncol. 2021 Oct;147(10):3081-3089. doi: 
            10.1007/s00432-021-03569-8. Epub 2021 Mar 7.
  PUBMED    33677711
REFERENCE   2  (bases 1 to 7776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00432-021-03569-8"; journalName: "J Cancer Res Clin 
            Oncol"; date: "2021-10"; volume: "147"; issue: "10"; pages: 
            "3081-3089"
COMMENT     Sequence Label: pUC18_BCR_GUSB_ABL1_e14a3
FEATURES             Location/Qualifiers
     source          1..7776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2823..2839)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2823..2839)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2836..2858)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2847..2863
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2871..2877,2878..2895,2896..2901))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2916..2937
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3054..3071)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3225..3813)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3305..3324)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3984..4775,4776..4844))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4607..4626
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4845..4949)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5017..5035
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5073..5095)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5195..5214
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5408..5430
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5422..5439
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5423..5439
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tccttggggt ctgcacccgg gagcccccgt tctatatcat cactgagttc atgacctacg
       61 ggaacctcct ggactacctg agggagtgca accggcagga ggtgaacgcc gtggtgctgc
      121 tgtacatggc cactcagatc tcgtcagcca tggagtacct ggagaagaaa aacttcatcc
      181 acagagatct tgctgcccga aactgcctgg taggggagaa ccacttggtg aaggtagctg
      241 attttggcct gagcaggttg atgacagggg acacctacac agcccatgct ggagccaagt
      301 tccccatcaa atggactgca cccgagagcc tggcctacaa caagttctcc atcaagtccg
      361 acgtctgggc atttggagta ttgctttggg aaattgctac ctatggcatg tccccttacc
      421 cgggaattga cctgtcccag gtgtatgagc tgctagagaa ggactaccgc atggagcgcc
      481 cagaaggctg cccagagaag gtctatgaac tcatgcgagc atgttggcag tggaatccct
      541 ctgaccggcc ctcctttgct gaaatccacc aagcctttga aacaatgttc caggaatcca
      601 gtatctcaga cgaagtggaa aaggagctgg ggaaacaagg cgtccgtggg gctgtgagta
      661 ccttgctgca ggccccagag ctgcccacca agacgaggac ctccaggaga gctgcagagc
      721 acagagacac cactgacgtg cctgagatgc ctcactccaa gggccaggga gagagcgatc
      781 ctctggacca tgagcctgcc gtgtctccat tgctccctcg aaaagagcga ggtcccccgg
      841 agggcggcct gaatgaagat gagcgccttc aagccgaatt ccagcacact ggcggccgtt
      901 actagatgca tgctcgagcg gccgccagtg tgatggatat ctgcagaatt cggcttgtaa
      961 acgggctgtt ttccaaacat tgtgacttgg ctactgagtg gggatacctg gtttcattgg
     1021 caatcttcca gtatctctct cgcaaaagga acgctgcact ttttggttgt ctctgccgag
     1081 tgaagatccc ctttttattc cccagcactc tcgtcggtga ctgttcagtc atgaaatcgg
     1141 caaaattcca aatgagctct ccaaccacgt attttctgcg tttttgatcc agacccagat
     1201 ggtactgctc tagcagactt ttctggtact cttcagtgaa catcagaggt ggatcctggt
     1261 gaaaccctgc aatcgtttct gctccatact cgctctgaat aatgggcttc tgatacttct
     1321 tataccagtt ctcaaactgg gtggccagct gcagctgaat caactccagg tgcccgtagt
     1381 cgtgatacca agagtagtag ctgttcaaac agatcacatc cacatacgga gcccccttgt
     1441 ctgctgcata gttagagttg ctcacaaagg tcacaggccg ggaggggtcc aaggatttgg
     1501 tgtgagcgat caccatcttc aagtagtagc cagcagattc taggtgggac gcaggctcgt
     1561 tggccacaga ccacatcacg accgcggggt ggttcttgtc cctacgcacc acttcttcca
     1621 tcacctgcat gtggtgatgc agagaaacgt tgttgaagaa ctgcggcagc gccaggccca
     1681 cgccgggaca ctcatcgatg accacaatcc catagcggtc acacatctgc atcacttcct
     1741 ctgcataggg gtagtggctg gtacggaaaa agccgaattc cagcacactg gcggccgtta
     1801 ctagtggatc cgagctcggt accgagctcg aattcggctt gtccactcag ccactggatt
     1861 taagcagagt tcaaatctgt actgcaccct ggaggtggat tcctttgggt attttgtgaa
     1921 taaagcaaag acgcgcgtct acagggacac agctgagcca aactggaacg aggaatttga
     1981 gatagagctg gagggctccc agaccctgag gatactgtgc tatgaaaagt gttacaacaa
     2041 gacgaagatc cccaaggagg acggcgagag cacggacaga ctcatgggga agggccaggt
     2101 ccagctggac ccgcaggccc tgcaggacag agactggcag cgcaccgtca tcgccatgaa
     2161 tgggatcgaa gtaaagctct cggtcaagtt caacagcagg gagttcagct tgaagaggat
     2221 gccgtcccga aaacagacag gggtcttcgg agtcaagatt gctgtggtca ccaagagaga
     2281 gaggtccaag gtgccctaca tcgtgcgcca gtgcgtggag gagatcgagc gccgaggcat
     2341 ggaggaggtg ggcatctacc gcgtgtccgg tgtggccacg gacatccagg cactgaaggc
     2401 agccttcgac gtcaataaca aggatgtgtc ggtgatgatg agcgagatgg acgtgaacgc
     2461 catcgcaggc acgctgaagc tgtacttccg tgagctgccc gagcccctct tcactgacga
     2521 gttctacccc aacttcgcag agggcatcgc tctttcagac ccggttgcaa aggagagctg
     2581 catgctcaac ctgctgctgt ccctgccgga ggccaacctg ctcaccttcc ttttccttct
     2641 ggaccacctg aaaagggtgg cagagaagga ggcagtcaat aagatgtccc tgcacaacct
     2701 cgccacggtc tttggcccca cgctgctccg gccctccgag aaggagagca agctccctgc
     2761 caaccccagc cagcctatca ccatgactga cagctggtcc ttgaagccga attcgtaatc
     2821 atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     2881 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     2941 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     3001 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     3061 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3121 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3181 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3241 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3301 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3361 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3421 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3481 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3541 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3601 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3661 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3721 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3781 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3841 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3901 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3961 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4021 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4081 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     4141 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4201 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4261 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     4321 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     4381 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     4441 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     4501 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     4561 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     4621 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     4681 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     4741 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     4801 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     4861 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     4921 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     4981 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     5041 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     5101 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     5161 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     5221 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     5281 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     5341 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     5401 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagcttgg taccgagctc
     5461 ggatccacta gtaacggccg ccagtgtgct ggaattcgcc cttcttcgtc tgagatactg
     5521 gattcctgga acattgtttc aaaggcttgg tggatttcag caaaggaggg ccggtcagag
     5581 ggattccact gccaacatgc tcgcatgagt tcatagacct tctctgggca gccttctggg
     5641 cgctccatgc ggtagtcctt ctctagcagc tcatacacct gggacaggtc aattcccggg
     5701 taaggggaca tgccataggt agcaatttcc caaagcaata ctccaaatgc ccagacgtcg
     5761 gacttgatgg agaacttgtt gtaggccagg ctctcgggtg cagtccattt gatggggaac
     5821 ttggctccag catgggctgt gtaggtgtcc cctgtcatca acctgctcag gccaaaatca
     5881 gctaccttca ccaagtggtt ctcccctacc aggcagtttc gggcagcaag atctctgtgg
     5941 atgaagtttt tcttctccag gtactccatg gctgacgaga tctgagtggc catgtacagc
     6001 agcaccacgg cgttcacctc ctgccggttg cactccctca ggtagtccag gaggttcccg
     6061 taggtcatga actcagtgat gatatagaac gggggctccc gggtgcagac cccaaggagc
     6121 tgcaccaggt tagggtgttt gatctctttc atgactgcag cttctttcaa gaactcttcc
     6181 acctccatgg tgtcctcctt caaggtcttc acggccaccg tcaggctgta tttcttccac
     6241 acgccctcgt acacctcccc gtactggccc ccgcccagct tgtgcttcat ggtgatgtcc
     6301 gtgcgttcca tctcccactt gtcgtagttg ggggacacac catagacagt gggcttgttg
     6361 cgctttgggg ctggataatg gagcgtggtg atgagcccgt cggccaccgt tgaatgatga
     6421 tgaaccaact cggccagggt gttgaagcgg ctctcggagg agacgtagag cttgccatca
     6481 gaagcagtgt tgatcctgta atggtacacc ctcccttcgt atctcagcga gatggacctc
     6541 tggccaggac tgctctcact ctcacgcacc aagaagctgc cattgatccc gctgctcagc
     6601 agatactcag cggcattgcg ggacacaggc ccatggtacc aggagtgttt ctccagactg
     6661 ttgactggcg tgatgtagtt gcttgggacc cagccttggc catttttggt ttgggcttca
     6721 caccattccc cattgtgatt atagcctaag acccggagct tttcaccttt agttatgctt
     6781 agagtgttat ctccactggc cacaaaatca tacagtgcaa cgaaaaggtt ggggtcattt
     6841 tcactgggtc cagcgagaag gttttccttg gagttccaac gaagccgaat tctgcagata
     6901 tccatcacac tggcggccgc tcgagcatgc atctagagtc gacctgcagg catgcaagct
     6961 tctccctgac atccgtggag ctgcagatgc tgaccaactc gtgtgtgaaa ctccagactg
     7021 tccacagcat tccgctgacc atcaataagg tagatgatga gtctccgggg ctctatgggt
     7081 ttctgaatgt catcgtccac tcagccactg gatttaagca gagttcaagt gaaaagctcc
     7141 gggtcttagg ctataatcac aatggggaat ggtgtgaagc ccaaaccaaa aatggccaag
     7201 gctgggtccc aagcaactac atcacgccag tcaacagtct ggagaaacac tcctggtacc
     7261 atgggcctgt gtcccgcaat gccgctgagt atctgctgag cagcgggatc aatggcagct
     7321 tcttggtgcg tgagagtgag agcagtcctg gccagaggtc catctcgctg agatacgaag
     7381 ggagggtgta ccattacagg atcaacactg cttctgatgg caagctctac gtctcctccg
     7441 agagccgctt caacaccctg gccgagttgg ttcatcatca ttcaacggtg gccgacgggc
     7501 tcatcaccac gctccattat ccagccccaa agcgcaacaa gcccactgtc tatggtgtgt
     7561 cccccaacta cgacaagtgg gagatggaac gcacggacat caccatgaag cacaagctgg
     7621 gcgggggcca gtacgggaag gtgtacgagg gcgtgtggaa gaaatacagc ctgacggtgg
     7681 ccgtgaagac cttgaaggag gacaccatgg aggtggaaga gttcttgaaa gaagctgcag
     7741 tcatgaaaga gatcaaacac cctaacctgg tgcagc
//
