LOCUS       sequence-414833-        3799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for plants with medium-copy number origin of 
            replication.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , 
            Nakayama, Naomi
  TITLE     Mobius Assembly for Plant Systems highlights promoter-terminator 
            interaction in gene regulation
  JOURNAL   bioRxiv, March 31, 2021
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 31, 2021"
COMMENT     Sequence Label: pLXMA L1A
FEATURES             Location/Qualifiers
     source          1..3799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    723..747
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    774..798
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        840..874
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             883..894
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1583..1654
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1670..1697
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1927..2742)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2734..2753
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(2868..3530)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      join(3531..3799,1..503)
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 ccccacgcct tcggcgcggc tccccctccg gcaaaaagtg gcccctccgg ggcttgttga
       61 tcgactgcgc ggccttcggc cttgcccaag gtggcgctgc ccccttggaa cccccgcact
      121 cgccgccgtg aggctcgggg ggcaggcggg cgggcttcgc ccttcgactg cccccactcg
      181 cataggcttg ggtcgttcca ggcgcgtcaa ggccaagccg ctgcgcggtc gctgcgcgag
      241 ccttgacccg ccttccactt ggtgtccaac cggcaagcga agcgcgcagg ccgcaggccg
      301 gaggcttttc cccagagaaa attaaaaaaa ttgatggggc aaggccgcag gccgcgcagt
      361 tggagccggt gggtatgtgg tcgaaggctg ggtagccggt gggcaatccc tgtggtcaag
      421 ctcgtgggca ggcgcagcct gtccatcagc ttgtccagca gggttgtcca cgggccgagc
      481 gaagcgagcc agccggtggc cgctcgcggc catcgtccac atatccacgg gctggcaagg
      541 gagcgcagcg accgcgcagg gcgaagcccg gagagcaagc ccgtaggggg ccatcatcag
      601 ttcggtggtc ttccgacgaa caataaggcc gcaaatcgcg gcctttttta ttgataacaa
      661 aaccggctca gttctgcgta gaaaccaaca tgcaagctcc accgggtgca aagcggcagc
      721 ggcggcagga tatattcaat tgtaaatggc ttcatgtccg ggaaatctac tggtggcagg
      781 atatattgtg gtgtaaacaa tggagaaaaa gagaattcac ctgcatatgg agagagacct
      841 ttacggctag ctcagtccta ggtatagtgc tagctactag agaaagagga gaaatactaa
      901 atgtcgcact caaaacaagc actggcagat acgatgaaaa tgacgtggct gatggaaggt
      961 tcggtcaacg gtcacgcctt tacgattgaa ggcgaaggta ccggcaaacc gtatgaaggt
     1021 aaacagagtg gcacctttcg tgttacgaaa ggcggtccgc tgccgtttgc gtttgatatt
     1081 gtcgctccga ccctgaaata cggtttcaaa tgcttcatga aatacccggc ggatattccg
     1141 gactacttta aactggcctt cccggaaggc ctgacgtacg atcgtaaaat tgcgtttgaa
     1201 gacggcggtt gtgcgaccgc cacggtcgaa atgagcctga agggtaacac cctggtgcat
     1261 aaaacgaact ttcagggcgg taatttcccg attgatggtc cggtgatgca aaaacgcacc
     1321 ctgggctggg aaccgacctc tgaaaaaatg acgccgtgcg atggtattat caaaggcgac
     1381 accatcatgt acctgatggt tgaaggcggt aaaacgctga aatgtcgtta tgaaaacaat
     1441 taccgcgcca acaaaccagt gctgatgccg ccgagccact ttgtggatct gcgtctgacc
     1501 cgcacgaatc tggataaaga aggtctggcg ttcaaactgg aagaatatgc tgttgcccgt
     1561 gtgctggaag tgtaataagc ttcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1621 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1681 gggtgggcct ttctgcgggt ctcacgctca gaatatgcag gtgctgcagg aattgttgat
     1741 tttgtgatga ctgatggcag gatatatgcg gttgtaattc atttttattg tctaaatttc
     1801 tgtatttgtt tgtttgttcg gttgtaaatt tttttggaag aacaagaaaa gaaaaaacac
     1861 ccgttagggt gtttttagtt agtgtggcgc gccgacttgc gacatgcggt cctttgcaat
     1921 caactattag aaaaattcat ccagcatcag atgaaattgc agtttgttca tatccggatt
     1981 atcaatgcca tatttctgaa acagacgttt ttgcaggctc gggctaaatt cgcccaggca
     2041 gttccacaga atggccagat cctgataacg atccgcaatg cccacacggc ccacatcaat
     2101 gcagccaatc agtttgcctt catcgaaaat caggttatcc aggctaaaat cgccgtgggt
     2161 caccacgcta tccgggctaa acggcagcag tttatgcatt tctttccaca cctgttccac
     2221 cggccagccg ttacgttcat catcaaaatc gctcgcatcc accaggccgt tgttcatacg
     2281 gctctgcgcc tgggccagac gaaacacacg atcgctgtta aacgggcagt tgcacaccgg
     2341 aatgctatgc agacgacgca gaaacacggc cagcgcatcc acaatgtttt cgccgctatc
     2401 cggatattct tccagcacct gaaacgcggt tttgcccgga atcgcggtgg tcagcagcca
     2461 cgcatcatcc ggggtgcgaa taaaatgttt aatggtcggc agcggcataa attcggtcag
     2521 ccagttcaga cgcaccattt catcggtcac atcgttcgcc acgctgcctt tgccatgttt
     2581 cagaaacagt tccggcgcat ccggtttgcc atacagacga taaatggtcg cgccgctctg
     2641 acccacgtta tcacgcgccc atttatagcc atacagatcc gcatccatgt tgctgttcag
     2701 acgcggacgg ctacagctcg tttcacgctg aatatggctc ataacacccc ttgtattact
     2761 gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta
     2821 acatcagaga ttttgagaca caagatcgga ttggcggtta tgcggttcta ccggcgcggc
     2881 agcgttaccc gtgtcggcgg ctccaacggc tcgccatcgt ccagaaaaca cggctcatcg
     2941 ggcatcggca ggcgctgctg cccgcgccgt tcccattcct ccgtttcggt caaggctggc
     3001 aggtctggtt ccatgcccgg aatgccgggc tggctgggcg gctcctcgcc ggggccggtc
     3061 ggtagttgct gctcgcccgg atacagggtc gggatgcggc gcaggtcgcc atgccccaac
     3121 agcgattcgt cctggtcgtc gtgatcaacc accacggcgg cactgaacac cgacaggcgc
     3181 aactggtcgc ggggctggcc ccacgccacg cggtcattga ccacgtaggc cgacacggtg
     3241 ccggggccgt tgagcttcac gacggagatc cagcgctcgg ccaccaagtc cttgactgcg
     3301 tattggaccg tccgcaaaga acgtccgatg agcttggaaa gtgtcttctg gctgaccacc
     3361 acggcgttct ggtggcccat ctgcgccacg aggtgatgca gcagcattgc cgccgtgggt
     3421 ttcctcgcaa taagcccggc ccacgcctca tgcgctttgc gttccgtttg cacccagtga
     3481 ccgggcttgt tcttggcttg aatgccgatt tctctggact gcgtggccat gcttatctcc
     3541 atgcggtagg ggtgccgcac ggttgcggca ccatgcgcaa tcagctgcaa cttttcggca
     3601 gcgcgacaac aattatgcgt tgcgtaaaag tggcagtcaa ttacagattt tctttaacct
     3661 acgcaatgag ctattgcggg gggtgccgca atgagctgtt gcgtaccccc cttttttaag
     3721 ttgttgattt ttaagtcttt cgcatttcgc cctatatcta gttctttggt gcccaaagaa
     3781 gggcacccct gcggggttc
//
