LOCUS       sequence-41489-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Borza CM, Chen X, Mathew S, Mont S, Sanders CR, Zent R, Pozzi A
  TITLE     Integrin {alpha}1{beta}1 promotes caveolin-1 dephosphorylation by 
            activating T cell protein-tyrosine phosphatase.
  JOURNAL   J Biol Chem. 2010 Dec 17;285(51):40114-24. Epub 2010 Oct 12.
  PUBMED    20940300
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2010-12-17"; volume: "285(51)"; pages: "40114-24. 
            Epub 2010 Oct 12"
COMMENT     Sequence Label: pBG100-TCPTP
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..94
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgggttct tctcaccatc accatcacca
       61 tggcagcagc ctggaagttc tgttccaggg gcccggatcc atgcccacca ccatcgagcg
      121 ggagttcgaa gagttggata ctcagcgtcg ctggcagccg ctgtacttgg aaattcgaaa
      181 tgagtcccat gactatcctc atagagtggc caagtttcca gaaaacagaa atcgaaacag
      241 atacagagat gtaagcccat atgatcacag tcgtgttaaa ctgcaaaatg ctgagaatga
      301 ttatattaat gccagtttag ttgacataga agaggcacaa aggagttaca tcttaacaca
      361 gggtccactt cctaacacat gctgccattt ctggcttatg gtttggcagc agaagaccaa
      421 agcagttgtc atgctgaacc gcattgtgga gaaagaatcg gttaaatgtg cacagtactg
      481 gccaacagat gaccaagaga tgctgtttaa agaaacagga ttcagtgtga agctcttgtc
      541 agaaagatgt gaagtcgtat tatacagtac atctactaca attagaaaat atcaatagtg
      601 gtgaaaccag aaacaatatc tcactttcat tatactacct ggccagattt tggagtccct
      661 gaatcaccag cttcatttct caatttcttg tttaaagtga gagaatctgg ctccttgaac
      721 cctgaccatg ggcctgcggt gatccactgt agtgcaggca ttgggcgctc tggcaccttc
      781 tctctggtag acacttgtct tgntttgatg gaaaaaggag atgatataac ataaaac
//
