LOCUS       sequence-414900-        9872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of Human HLX in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Henriques-Oliveira L, Altman AR, Kurochkin I, Ascic E, Halitzki E, 
            Matei A, Pertiga-Cabral D, Ulmert I, Holst S, Nair MS, Cunha PP, 
            Park S, Vergani S, Kharas MG, Yuan J, Lahl K, Rosa FF, Pires CF, 
            Pereira C-F
  TITLE     Anchored screening identifies transcription factor blueprints 
            underlying dendritic cell diversity and subset-specific anti-tumor 
            immunity
  JOURNAL   Immunity. 58, 1-20. October 2025.
REFERENCE   2  (bases 1 to 9872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            58, 1-20. October 2025."
COMMENT     Sequence Label: pFUW-TetO-HLX
FEATURES             Location/Qualifiers
     source          1..9872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2403..2421,2422..2444,2445..2463,2464..2486,2487..2505,
                     2506..2528,2529..2547,2548..2570,2571..2589,2590..2612,
                     2613..2631,2632..2654,2655..2673)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2706..2744
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2741..2765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4353..4941
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4406..4426)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4824..4835)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4944..4960)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4945..4961)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5466..5646
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5672..5689)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5678..5902
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5948..6376
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6035..6054)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6245..6266
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6385..6405)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6390..6719
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6570..6705
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6632..6651
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6767..6814
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6833..7207
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7337..7458
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7374..7393)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7428..7447
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7507..7523)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7507..7523)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7520..7542)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7531..7547
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7555..7561,7562..7579,7580..7585))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7600..7621
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7738..7755)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7909..8497)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7989..8008)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8668..9459,9460..9528))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9291..9310
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9529..9633)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9708..9727)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     2401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2461 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2521 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2581 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg
     2701 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     2761 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     2821 ccagcctccg cggccccgaa ttcaccggtt ctagagctag catgttcgca gccgggctgg
     2881 ctcccttcta cgcctccaac ttcagcctct ggtcggccgc ttactgctcc tcggccggcc
     2941 caggcggctg ctccttcccc ttggaccccg ccgccgtcaa aaagccctcc ttctgcatcg
     3001 cagacattct gcacgccggc gtgggggatc tgggggcggc cccggagggc ctggcagggg
     3061 cctcggccgc cgccctcacc gcgcacttgg gctcggttca cccgcacgcc tctttccaag
     3121 cggcggccag atccccgctt cgacccaccc cagtggtggc gccctccgaa gtcccggctg
     3181 gcttcccgca gcggctgtct ccgctctcag ccgcctacca ccaccatcac ccgcaacaac
     3241 aacagcagca gcaacagccg cagcagcaac agcctccgcc tccgccccgg gctggcgccc
     3301 tgcagccccc ggcctcgggg acgcgagtgg ttccgaaccc ccaccacagt ggctctgccc
     3361 cggccccctc cagcaaagac ctcaaatttg gaattgaccg cattttatct gcagaatttg
     3421 acccaaaagt caaagaaggc aacacgctga gagatctcac ttccctgcta accggtgggc
     3481 ggcccgccgg ggtgcacctc tcaggcctgc agccctcggc cggccagttc ttcgcatctc
     3541 tagatcccat taacgaggct tctgcaatcc tgagtccctt aaactcgaac ccaagaaatt
     3601 cagttcagca tcagttccaa gacacgtttc caggtcccta tgctgtgctc acgaaggaca
     3661 ccatgccgca gacgtacaaa aggaagcgtt catggtcgcg cgctgtgttc tccaacctgc
     3721 agaggaaagg cctggagaaa aggtttgaga ttcagaagta cgtgaccaag ccggaccgaa
     3781 agcagctggc ggcgatgctg ggcctcacgg acgcacaggt gaaggtgtgg ttccagaacc
     3841 ggcggatgaa gtggcggcac tccaaggagg cccaggccca aaaggacaag gacaaggagg
     3901 ctggcgagaa gccatcaggt ggagccccgg ctgcggatgg cgagcaggac gagaggagcc
     3961 ccagccgttc tgaaggcgag gctgagagcg agagcagcga ctccgagtcc ctggacatgg
     4021 cccccagcga cacggagcgg actgagggga gtgagcgttc tctgcaccaa acaacagtta
     4081 ttaaggcccc ggtcactggc gccctcatta ccgccagcag tgctgggagt ggtgggagca
     4141 gcggcggcgg cggcaatagt ttcagcttca gcagcgccag cagtcttagt agcagcagca
     4201 ccagtgcggg ttgcgccagc agccttggcg gcggcggcgc ctcggagctt ctccctgcaa
     4261 cacagcccac agccagcagc gctcccaaaa gccccgagcc agcccaaggc gcgcttggct
     4321 gcttatagtt cgaagatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     4381 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     4441 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     4501 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     4561 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     4621 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     4681 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     4741 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     4801 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     4861 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     4921 ccctttgggc cgcctccccg catcgatacc gtcgacctcg agacctagaa aaacatggag
     4981 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
     5041 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca
     5101 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     5161 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     5221 ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt
     5281 gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca
     5341 cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag
     5401 agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact
     5461 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     5521 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     5581 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     5641 ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     5701 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     5761 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     5821 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     5881 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     5941 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     6001 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     6061 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     6121 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     6181 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     6241 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     6301 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6361 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     6421 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     6481 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6541 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     6601 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     6661 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     6721 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
     6781 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
     6841 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
     6901 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
     6961 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
     7021 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
     7081 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
     7141 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
     7201 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     7261 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     7321 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7381 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7441 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     7501 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7561 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7621 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7681 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     7741 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7801 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7861 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7921 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7981 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8041 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8101 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8161 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8221 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8281 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8341 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8401 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8461 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8521 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8581 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8641 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8701 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8761 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8821 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8881 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8941 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9001 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9061 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9121 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9181 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9241 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9301 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9361 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9421 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9481 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9541 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9601 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9661 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     9721 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     9781 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     9841 ctgcttaggg ttaggcgttt tgcgctgctt cg
//
