LOCUS       sequence-414901-       10611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For PYL-DNMT1 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10611)
  AUTHORS   Yang B, Borgeaud AC, Buricova M, Aeschbach L, Rodriguez-Lima O, Ruiz
            Buendia GA, Cinesi C, Taylor AS, Baubec T, Dion V
  TITLE     Expanded CAG/CTG repeats resist gene silencing mediated by targeted 
            Epigenome editing.
  JOURNAL   Hum Mol Genet. 2021 Sep 7. pii: 6365982. doi: 10.1093/hmg/ddab255.
  PUBMED    34494094
REFERENCE   2  (bases 1 to 10611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2021 Sep 7. pii: 6365982. doi: 10.1093/hmg/ddab255."
COMMENT     Sequence Label: pAB(EXPR-PYL-DNMT1-NEO)
FEATURES             Location/Qualifiers
     source          1..10611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      679..688
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1222..1293
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     CDS             1297..1317
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    1318..1342
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5881..5905)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(5930..5948)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5931..5948)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5968..5985)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5974..6198
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6244..6672
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6331..6350)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6541..6562
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6681..6701)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6686..7015
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6866..7001
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6928..6947
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7082..7876
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7136..7155)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7746..7765
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8050..8171
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8087..8106)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8141..8160
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8220..8236)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8220..8236)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8233..8255)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8244..8260
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8268..8274,8275..8292,8293..8298))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8313..8334
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8451..8468)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8622..9210)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8702..8721)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9381..10172,10173..10241))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10004..10023
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10242..10346)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10421..10440)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccgc caccatggcc acccaggacg agttcaccca gctgagccag
      721 tctatcgccg agttccacac ctaccagctg ggcaacggcc ggtgcagcag cctgctggcc
      781 cagagaatcc acgcccctcc cgagacagtg tggtccgtcg tgcggagatt cgaccggccc
      841 cagatctaca agcacttcat caagagctgc aacgtgtccg aggacttcga gatgagagtg
      901 ggctgcaccc gggacgtgaa cgtgatcagc ggcctgcccg ccaacaccag cagagagaga
      961 ctggacctgc tggacgacga cagaagagtg accggcttca gcatcaccgg cggagagcac
     1021 cggctgcgga actacaagag cgtgaccacc gtgcacagat tcgagaaaga ggaagaagag
     1081 gaacggatct ggaccgtggt gctggaaagc tacgtggtgg acgtgcccga gggcaacagc
     1141 gaagaggaca cccggctgtt cgccgacacc gtgatccggc tgaacctgca gaagctggcc
     1201 tccatcaccg aggccatgaa cgactacaag gacgacgacg acaaggacta taaggacgat
     1261 gatgataagg attacaaaga tgacgacgat aagcccccca agaagaagcg gaaagtcaca
     1321 agtttgtaca aaaaagcagg ctcaatggca gatgccaaca gcccccccaa acccctttcc
     1381 aaacctcgca cgcccaggag gagcaagtcc gatggagagg ctaagcgttc aagagaccct
     1441 cctgcctcag cctcccaagt aactgggatt agagctgaac cttcacctag ccccaggatt
     1501 acaaggaaaa gcaccaggca aaccaccatc acatctcatt ttgcaaaggg ccctgccaaa
     1561 cggaaacctc aggaagagtc tgaaagagcc aaatcggatg agtccatcaa ggaagaagac
     1621 aaagaccagg atgagaagag acgtagagtt acatccagag aacgagttgc tagaccgctt
     1681 cctgcagaag aacctgaaag agcaaaatca ggaacgcgca ctgaaaagga agaagaaaga
     1741 gatgaaaaag aagaaaagag actccgaagt caaaccaaag aaccaacacc caaacagaaa
     1801 ctgaaggagg agccggacag agaagccagg gcaggcgtgc aggctgacga ggacgaagat
     1861 ggagacgaga aagatgagaa gaagcacaga agtcaaccca aagatctagc tgccaaacgg
     1921 aggcccgaag aaaaagaacc tgaaaaagta aatccacaga tttctgatga aaaagacgag
     1981 gatgaaaagg aggagaagag acgcaaaacg acccccaaag aaccaacgga gaaaaaaatg
     2041 gctcgcgcca aaacagtcat gaactccaag acccaccctc ccaagtgcat tcagtgcggg
     2101 cagtacctgg acgaccctga cctcaaatat gggcagcacc caccagacgc ggtggatgag
     2161 ccacagatgc tgacaaatga gaagctgtcc atctttgatg ccaacgagtc tggctttgag
     2221 agttatgagg cgcttcccca gcacaaactg acctgcttca gtgtgtactg taagcacggt
     2281 cacctgtgtc ccatcgacac cggcctcatc gagaagaata tcgaactctt cttttctggt
     2341 tcagcaaaac caatctatga tgatgaccca tctcttgaag gtggtgttaa tggcaaaaat
     2401 cttggcccca taaatgaatg gtggatcact ggctttgatg gaggtgaaaa ggccctcatc
     2461 ggcttcagca cctcatttgc cgaatacatt ctgatggatc ccagtcccga gtatgcgccc
     2521 atatttgggc tgatgcagga gaagatctac atcagcaaga ttgtggtgga gttcctgcag
     2581 agcaattccg actcgaccta tgaggacctg atcaacaaga tcgagaccac ggttcctcct
     2641 tctggcctca acttgaaccg cttcacagag gactccctcc tgcgacacgc gcagtttgtg
     2701 gtggagcagg tggagagtta tgacgaggcc ggggacagtg atgagcagcc catcttcctg
     2761 acgccctgca tgcgggacct gatcaagctg gctggggtca cgctgggaca gaggcgagcc
     2821 caggcgaggc ggcagaccat caggcattct accagggaga aggacagggg acccacgaaa
     2881 gccaccacca ccaagctggt ctaccagatc ttcgatactt tcttcgcaga gcaaattgaa
     2941 aaggatgaca gagaagacaa ggagaacgcc tttaagcgcc ggcgatgtgg cgtctgtgag
     3001 gtgtgtcagc agcctgagtg tgggaaatgt aaagcctgca aggacatggt taaatttggt
     3061 ggcagtggac ggagcaagca ggcttgccaa gagcggaggt gtcccaatat ggccatgaag
     3121 gaggcagatg acgatgagga agtcgatgat aacatcccag agatgccgtc acccaaaaaa
     3181 atgcaccagg ggaagaagaa gaaacagaac aagaatcgca tctcttgggt cggagaagcc
     3241 gtcaagactg atgggaagaa gagttactat aagaaggtgt gcattgatgc ggaaaccctg
     3301 gaagtggggg actgtgtctc tgttattcca gatgattcct caaaaccgct gtatctagca
     3361 agggtcacgg cgctgtggga ggacagcagc aacgggcaga tgtttcacgc ccactggttc
     3421 tgcgctggga cagacacagt cctcggggcc acgtcggacc ctctggagct gttcttggtg
     3481 gatgaatgtg aggacatgca gctttcatat atccacagca aagtgaaagt catctacaaa
     3541 gccccctccg aaaactgggc catggaggga ggcatggatc ccgagtccct gctggagggg
     3601 gacgacggga agacctactt ctaccagctg tggtatgatc aagactacgc gagattcgag
     3661 tcccctccaa aaacccagcc aacagaggac aacaagttca aattctgtgt gagctgtgcc
     3721 cgtctggctg agatgaggca aaaagaaatc cccagggtcc tggagcagct cgaggacctg
     3781 gatagccggg tcctctacta ctcagccacc aagaacggca tcctgtaccg agttggtgat
     3841 ggtgtgtacc tgccccctga ggccttcacg ttcaacatca agctgtccag tcccgtgaaa
     3901 cgcccacgga aggagcccgt ggatgaggac ctgtacccag agcactaccg gaaatactcc
     3961 gactacatca aaggcagcaa cctggatgcc cctgagccct accgaattgg ccggatcaaa
     4021 gagatcttct gtcccaagaa gagcaacggc aggcccaatg agactgacat caaaatccgg
     4081 gtcaacaagt tctacaggcc tgagaacacc cacaagtcca ctccagcgag ctaccacgca
     4141 gacatcaacc tgctctactg gagcgacgag gaggccgtgg tggacttcaa ggctgtgcag
     4201 ggccgctgca ccgtggagta tggggaggac ctgcccgagt gcgtccaggt gtactccatg
     4261 ggcggcccca accgcttcta cttcctcgag gcctataatg caaagagcaa aagctttgaa
     4321 gatcctccca accatgcccg tagccctgga aacaaaggga agggcaaggg aaaagggaag
     4381 ggcaagccca agtcccaagc ctgtgagccg agcgagccag agatagagat caagctgccc
     4441 aagctgcgga ccctggatgt gttttctggc tgcggggggt tgtcggaggg attccaccaa
     4501 gcaggcatct ctgacacgct gtgggccatc gagatgtggg accctgcggc ccaggcgttc
     4561 cggctgaaca accccggctc cacagtgttc acagaggact gcaacatcct gctgaagctg
     4621 gtcatggctg gggagaccac caactcccgc ggccagcggc tgccccagaa gggagacgtg
     4681 gagatgctgt gcggcgggcc gccctgccag ggcttcagcg gcatgaaccg cttcaattcg
     4741 cgcacctact ccaagttcaa aaactctctg gtggtttcct tcctcagcta ctgcgactac
     4801 taccggcccc ggttcttcct cctggagaat gtcaggaact ttgtctcctt caagcgctcc
     4861 atggtcctga agctcaccct ccgctgcctg gtccgcatgg gctatcagtg caccttcggc
     4921 gtgctgcagg ccggtcagta cggcgtggcc cagactagga ggcgggccat catcctggcc
     4981 gcggcccctg gagagaagct ccctctgttc ccggagccac tgcacgtgtt tgctccccgg
     5041 gcctgccagc tgagcgtggt ggtggatgac aagaagtttg tgagcaacat aaccaggttg
     5101 agctcgggtc ctttccggac catcacggtg cgagacacga tgtccgacct gccggaggtg
     5161 cggaatggag cctcggcact ggagatctcc tacaacgggg agcctcagtc ctggttccag
     5221 aggcagctcc ggggcgcaca gtaccagccc atcctcaggg accacatctg taaggacatg
     5281 agtgcattgg tggctgcccg catgcggcac atccccttgg ccccagggtc agactggcgc
     5341 gatctgccca acatcgaggt gcggctctca gacggcacca tggccaggaa gctgcggtat
     5401 acccaccatg acaggaagaa cggccgcagc agctctgggg ccctccgtgg ggtctgctcc
     5461 tgcgtggaag ccggcaaagc ctgcgacccc gcagccaggc agttcaacac cctcatcccc
     5521 tggtgcctgc cccacaccgg gaaccggcac aaccactggg ctggcctcta tggaaggctc
     5581 gagtgggacg gcttcttcag cacaaccgtc accaaccccg agcccatggg caagcagggc
     5641 cgcgtgctcc acccagagca gcaccgtgtg gtgagcgtgc gggagtgtgc ccgctcccag
     5701 ggcttccctg acacctaccg gctcttcggc aacatcctgg acaagcaccg gcaggtgggc
     5761 aatgccgtgc caccgcccct ggccaaagcc attggcttgg agatcaagct ttgtatgttg
     5821 gccaaagccc gagagagtgc ctcagctaaa ataaaggagg aggaagctgc taaggactag
     5881 acccagcttt cttgtacaaa gtggtcatcc gcggtctaga gggccctatt ctatagtgtc
     5941 acctaaatgc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     6001 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     6061 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     6121 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     6181 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     6241 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     6301 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     6361 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     6421 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     6481 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     6541 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     6601 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     6661 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     6721 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     6781 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     6841 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     6901 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     6961 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     7021 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
     7081 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     7141 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     7201 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     7261 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     7321 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     7381 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     7441 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     7501 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     7561 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     7621 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     7681 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     7741 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     7801 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     7861 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     7921 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     7981 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     8041 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     8101 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     8161 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     8221 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     8281 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     8341 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     8401 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     8461 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8521 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8581 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8641 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8701 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8761 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8821 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8881 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8941 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     9001 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     9061 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9121 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     9181 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     9241 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9301 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     9361 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     9421 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9481 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9541 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9601 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9661 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9721 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9781 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9841 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9901 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9961 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    10021 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    10081 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    10141 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    10201 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    10261 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    10321 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
    10381 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
    10441 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
    10501 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
    10561 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt t
//
