LOCUS       sequence-415031-       10205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of Mouse Nr4a1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Henriques-Oliveira L, Altman AR, Kurochkin I, Ascic E, Halitzki E, 
            Matei A, Pertiga-Cabral D, Ulmert I, Holst S, Nair MS, Cunha PP, 
            Park S, Vergani S, Kharas MG, Yuan J, Lahl K, Rosa FF, Pires CF, 
            Pereira C-F
  TITLE     Anchored screening identifies transcription factor blueprints 
            underlying dendritic cell diversity and subset-specific anti-tumor 
            immunity
  JOURNAL   Immunity. 58, 1-20. October 2025.
REFERENCE   2  (bases 1 to 10205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            58, 1-20. October 2025."
COMMENT     Sequence Label: pFUW-TetO-Nr4a1
FEATURES             Location/Qualifiers
     source          1..10205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2403..2421,2422..2444,2445..2463,2464..2486,2487..2505,
                     2506..2528,2529..2547,2548..2570,2571..2589,2590..2612,
                     2613..2631,2632..2654,2655..2673)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2706..2744
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2741..2765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4686..5274
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4739..4759)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5157..5168)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5277..5293)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5278..5294)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5799..5979
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6005..6022)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6011..6235
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6281..6709
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6368..6387)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6578..6599
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6718..6738)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6723..7052
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6903..7038
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6965..6984
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7100..7147
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7166..7540
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7670..7791
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7707..7726)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7761..7780
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7840..7856)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7840..7856)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7853..7875)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7864..7880
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7888..7894,7895..7912,7913..7918))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7933..7954
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8071..8088)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8242..8830)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8322..8341)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9001..9792,9793..9861))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9624..9643
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9862..9966)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10041..10060)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     2401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2461 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2521 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2581 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg
     2701 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     2761 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     2821 ccagcctccg cggccccgaa ttcaccggtt ctagaatgcc ctgtattcaa gctcaatatg
     2881 gaacaccagc aacgagccca ggaccgcgtg accacctgac cggtgatccc ctggcccttg
     2941 agttcggcaa gcctaccatg gacctggcca gccccgagac agcacctgcc gcacctgcta
     3001 cactgcccag cttcagcacc ttcatggacg ggtacaccgg agagtttgac accttcctct
     3061 accagctgcc ggggacgacc cagccgtgct cctcagcttg ttcctctgcc tcctccacgt
     3121 cttcttcctc atcctcggcc acctcccccg cttcggcgtc cttcaagttt gaggacttcc
     3181 aggtgtacgg ctgctacccg ggcaccctga gcggcccatt agatgagacc ctatcctcca
     3241 gcggctctga gtactatggc agtccctgct cagccccctc gccatctaca cccaacttcc
     3301 agccgtccca gctttctccc tgggacggct catttggcca cttctccccg agccagactt
     3361 atgaaggcct ctgggcatgg acagagcagt tgcctaaggc ttcttcaggg cctccgccac
     3421 ctccaacctt cttctccttc agtcctccca ctggccccag ccccagcctg gcccagagtt
     3481 ctctgaaatt gttcccacca ccagccaccc accagcttgg ggagggggag agctattcca
     3541 tgccagcagc tttccccggc ttggcaccca cctctccgaa ccgtgacact tccggcattc
     3601 tggacgcacc cgtgacctcc accaagtccc ggagcggggc ttcaggtggc agcgagggcc
     3661 gctgtgcagt ctgtggtgac aatgcttcgt gtcagcacta tggggtccgc acctgtgagg
     3721 gctgcaaggg cttcttcaag cgcacagtac agaaaagcgc caagtacatc tgcctggcaa
     3781 acaaggattg ccctgtggac aagaggcggc ggaaccgctg ccagttctgc cgcttccaga
     3841 agtgcctggc tgtgggcatg gtgaaggaag ttgtacggac agacagccta aaagggcggc
     3901 ggggccggct accttcaaaa cccaagcagc ctccagatgc ctcccctacc aatcttctca
     3961 cttccctcat ccgggcacac ttggactccg ggcctagcac tgccaaattg gactattcca
     4021 agttccagga actggtgctg ccccgcttcg ggaaggaaga tgccggtgac gtgcaacaat
     4081 tttatgactt gctctctggt tccctggacg ttatccgaaa gtgggcagaa aaaatccctg
     4141 gcttcattga gctttgccca ggagaccaag acctgttgct agagtctgcc ttcctggaac
     4201 tcttcatcct ccgcctggca taccgatcta aacccggtga ggggaagctc atcttctgct
     4261 caggcctggt actacaccag ctgcagtgtg cccgtggctt tggtgattgg attgacaaca
     4321 tcctggcctt ctcacggtcc ctgcacagct tgggtgttga tgttcccgcc tttgcctgcc
     4381 tgtccgctct ggtcctcatc actgatcgac acgggctcca ggaccctcgt cgggtggaag
     4441 agctgcagaa tcgcattgct agctgtctga aggagcacat ggctaccgtg gcaggagacc
     4501 cacagccggc cagctgcctg tcacgtctgc tgggcaaact gcctgagctt cggaccctgt
     4561 gcactcaagg cctgcagcgc atcttttgcc tcaagttgga ggacttggta ccccctccac
     4621 ctattgtgga caagatcttt atggacacat tgtctttctg attcgaagat atcaagctta
     4681 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     4741 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4801 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     4861 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     4921 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     4981 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     5041 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     5101 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     5161 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     5221 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     5281 accgtcgacc tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     5341 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     5401 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     5461 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     5521 atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc
     5581 cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag
     5641 agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc
     5701 atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat
     5761 ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga
     5821 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     5881 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     5941 ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga
     6001 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     6061 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     6121 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     6181 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     6241 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     6301 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6361 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6421 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6481 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6541 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6601 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6661 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     6721 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6781 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     6841 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     6901 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6961 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     7021 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     7081 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
     7141 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
     7201 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
     7261 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
     7321 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
     7381 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
     7441 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
     7501 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
     7561 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     7621 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     7681 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7741 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     7801 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     7861 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7921 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7981 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8041 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8101 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8161 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8221 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8281 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8341 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8401 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8461 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8521 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8581 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8641 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     8701 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8761 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8821 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8881 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8941 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9001 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9061 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9961 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
    10021 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
    10081 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
    10141 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
    10201 cttcg
//
