LOCUS       sequence-415036-       11839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Premade nuclei membrane marker, pUBQ10:H2B:mVenus:tHSP18.2M:FastRed,
            for braiding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11839)
  AUTHORS   Piepers M, Erbstein K, Reyes-Hernandez J, Song C, Tessi T, 
            Petrasiunaite V, Faerber N, Distel K, Maizel A
  TITLE     GreenGate 2.0: Backwards compatible addons for assembly of complex 
            transcriptional units and their stacking with GreenGate.
  JOURNAL   PLoS One. 2023 Sep 8;18(9):e0290097. doi: 
            10.1371/journal.pone.0290097. eCollection 2023.
  PUBMED    37682951
REFERENCE   2  (bases 1 to 11839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2023-09-8"; volume: "18(9):e0290097. doi"; pages: " 
            10.1371/journal.pone.0290097. eCollection 2023"
COMMENT     Sequence Label: pKD003
FEATURES             Location/Qualifiers
     source          1..11839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1389..1391,1392..1394,1395..2108)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1434..1455)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1695..1714)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2042..2063
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4520..5227
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MLSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     4933..4952
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5504..5528
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6829..7458
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7887..8960
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9026..9220
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9456..9478)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9564..9704
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9619..9638
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9719..9736)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9890..10478)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9970..9989)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10566..11381)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(10566..11381)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     11289..11308
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    11814..11838
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 taacgaatta ccatggcacc tgctgcaacc tcgacgagtc agtaataaac ggcgtcaaag
       61 tggttgcagc cggcacacac gagtcgtgtt tatcaactca aagcacaaat acttttcctc
      121 aacctaaaaa taaggcaatt agccaaaaac aactttgcgt gtaaacaacg ctcaatacac
      181 gtgtcatttt attattagct attgcttcac cgccttagct ttctcgtgac ctagtcgtcc
      241 tcgtcttttc ttcttcttct tctataaaac aatacccaaa gagctcttct tcttcacaat
      301 tcagatttca atttctcaaa atcttaaaaa ctttctctca attctctcta ccgtgatcaa
      361 ggtaaatttc tgtgttcctt attctctcaa aatcttcgat tttgttttcg ttcgatccca
      421 atttcgtata tgttctttgg tttagattct gttaatctta gatcgaagac gattttctgg
      481 gtttgatcgt tagatatcat cttaattctc gattagggtt tcatagatat catccgattt
      541 gttcaaataa tttgagtttt gtcgaataat tactcttcga tttgtgattt ctatctagat
      601 ctggtgttag tttctagttt gtgcgatcga atttgtagat taatctgagt ttttctgatt
      661 aacaaacaat ggcaccaaaa gccggaaaga aaccagcaga gaaaaagccc gcagagaaag
      721 ctccagcaga agaagagaaa gtagccgaaa aagcaccagc agagaagaaa ccaaaagccg
      781 gaaaaaaact accaaaagaa gcagtcaccg gaggagtcga gaagaagaag aaacgagtca
      841 agaaaagcac agagacatac aagatctaca tcttcaaggt gttgaaacaa gttcatccag
      901 atattggaat ctcaagtaaa gctatgggga ttatgaacag ttttatcaat gatatattcg
      961 agaaacttgc gcaagaagct tcaaaattag ctaggtataa taagaaacct acgattactt
     1021 ctagggagat tcagacagct gttagactag tgttacctgg tgaacttgct aagcatgctg
     1081 tttctgaagg aactaaggct gttactaagt ttactagctc ttcaggttcg acagccgcta
     1141 gtgcgtctgc atcagggagt ggttccggaa gcggctctgg atcgggctca gggagtggtt
     1201 ccggcagcgg ctctggatcg gcggccgctg caggctccgc tgctgcttca ggttctggct
     1261 ccggatctgg ctcaggtagt gggtccggat caggatcagg ttcgggttca gggtccggat
     1321 ctggttccgg ctcttcaagt tcaggtgcta gcggtggcag cggtggcact agtggtggag
     1381 gcggatccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1441 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     1501 ccacctacgg caagctgacc ctgaagctga tctgcaccac cggcaagctg cccgtgccct
     1561 ggcccaccct cgtgaccacc ctgggctacg gcctgcagtg cttcgcccgc taccccgacc
     1621 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1681 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     1741 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1801 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcacc gccgacaagc
     1861 agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcggcgtgc
     1921 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     1981 acaaccacta cctgagctac cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc
     2041 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     2101 acaagtagct gcgagctcat atgaagatga agatgaaata tttggtgtgt caaataaata
     2161 gcttgtgtgc ttaagtttgt gtttttttct tggcttgttg tgttatgaat ttgtggcttt
     2221 ttctaatatt aaatgaatgt aagatctcat tataatgaat aaacaaatgt ttctataatc
     2281 cattgtgaat gttttgttgg atctcttctg cagcatataa ctactgtatg tgctatggta
     2341 tggactatgg aatatgatta aagataagga attcactact tcaagtgtat gtaggtatag
     2401 taacatgaac aagaacagac tcaagtacaa gatcgcatac gaaaatggaa atggcaatgt
     2461 cacttccaca taatcaaaca cgaatcctca tatcaacaag gcctgagatt ctaactagct
     2521 cataacaact tagccaatag ttacttgaga ctaccaaatg tatgtagaac taaagactaa
     2581 gggacagaga gttcgtctaa acaggtgaat ctagtcgttg ttatctaata aacaattcag
     2641 ccccaaatgc agaacacaca tagagctctc tattgattca aattacgatc tgatactgat
     2701 aacgtctaga tttttagggt taaagcaatc aatcacctga cgattcaagg tggttggatc
     2761 atgacgattc cagaaaacat caagcaagct ctcaaagcta cactctttgg gatcatactg
     2821 aactctaaca acctcgttat gtcccgtagt gccagtacag acatcctcgt aactcggatt
     2881 gtgcacgatg ccatggctat acccaacctc ggtcttggtc acaccaggaa ctctctggta
     2941 agctagctcc actccccaga aacaaccggc gccaaattgc gcgaattgct gacctgaaga
     3001 cggaacatca tcgtcgggtc cttgggcgat tgcggcggaa gatgggtcag cttgggcttg
     3061 aggaccagac ccgaatccga gtctgttgaa aaggttgttc attggggatt tgtatacgga
     3121 gattggtcgt cgagaggttt gagggaaagg acaaatgggt ttggctctgg agaaagagag
     3181 tgcggcttta gagagagaat tgagaggttt agagagagat gcggcggcga tgagcggagg
     3241 agagacgacg aggacctgca ttatcaaagc agtgacgtgg tgaaatttgg aacttttaag
     3301 aggcagatag atttattatt tgtatccatt ttcttcattg ttctagaatg tcgcggaaca
     3361 aattttaaaa ctaaatccta aatttttcta attttgttgc caatagtgga tatgtgggcc
     3421 gtatagaagg aatctattga aggcccaaac ccatactgac gagcccaaag gttcgttttg
     3481 cgttttatgt ttcggttcga tgccaacgcc acattctgag ctaggcaaaa aacaaacgtg
     3541 tctttgaata gactcctctc gttaacacat gcagcggctg catggtgacg ccattaacac
     3601 gtggcctaca attgcatgat gtctccattg acacgtgact tctcgtctcc tttcttaata
     3661 tatctaacaa acactcctac ctcttccaaa atatatacac atctttttga tcaatctctc
     3721 attcaaaatc tcattctctc tagtaaacaa gaacaaaaaa atggcggata cagctagagg
     3781 aacccatcac gatatcatcg gcagggacca gtacccgatg atgggccgag atcgagatca
     3841 gtaccagatg tccggacgag gatctgacta ctccaagtct aggcagattg ctaaagctgc
     3901 aactgctgtc acagctggtg gttccctcct tgttctctcc agccttaccc ttgttggaac
     3961 tgtcatagct ttgactgttg caacacctct gctcgttatc ttcagcccaa tccttgtccc
     4021 ggctctcatc acagttgcac tcctcatcac cggttttctt tcctctggag ggtttggcat
     4081 tgccgctata accgttttct cttggattta caagtaagca cacatttatc atcttacttc
     4141 ataattttgt gcaatatgtg catgcatgtg ttgagccagt agctttggat caattttttt
     4201 ggtcgaataa caaatgtaac aataagaaat tgcaaattct agggaacatt tggttaacta
     4261 aatacgaaat ttgacctagc tagcttgaat gtgtctgtgt atatcatcta tataggtaaa
     4321 atgcttggta tgatacctat tgattgtgaa taggtacgca acgggagagc acccacaggg
     4381 atcagacaag ttggacagtg caaggatgaa gttgggaagc aaagctcagg atctgaaaga
     4441 cagagctcag tactacggac agcaacatac tggtggggaa catgaccgtg accgtactcg
     4501 tggtggccag cacactacta tgttgagcaa gggcgaggag gataacatgg ccatcatcaa
     4561 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     4621 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     4681 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     4741 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     4801 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     4861 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     4921 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     4981 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     5041 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     5101 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     5161 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     5221 gtacaagcat atgtgagtat ctagagtcaa gcagatcgtt caaacatttg gcaataaagt
     5281 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     5341 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     5401 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     5461 aactaggata aagtatgtca tatggcaggt gctcgagcaa ttctgacagg atatattggc
     5521 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
     5581 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
     5641 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
     5701 agccgtcatc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
     5761 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
     5821 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
     5881 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
     5941 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
     6001 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
     6061 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
     6121 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
     6181 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
     6241 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     6301 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     6361 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     6421 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     6481 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     6541 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     6601 cgggtacgtg ttcgagccgc ccgcgcacct ctcaaccgtg cggctgcatg aaatcctggc
     6661 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     6721 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     6781 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     6841 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     6901 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     6961 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     7021 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     7081 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     7141 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     7201 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     7261 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     7321 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     7381 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     7441 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     7501 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     7561 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     7621 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
     7681 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     7741 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     7801 ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact
     7861 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
     7921 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
     7981 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
     8041 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
     8101 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
     8161 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
     8221 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
     8281 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
     8341 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
     8401 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
     8461 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
     8521 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
     8581 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
     8641 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
     8701 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
     8761 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
     8821 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
     8881 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
     8941 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
     9001 aaaaaggtcg aaaaggactc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
     9061 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
     9121 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
     9181 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
     9241 ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
     9301 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
     9361 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
     9421 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggtg
     9481 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     9541 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     9601 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     9661 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     9721 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     9781 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     9841 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     9901 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     9961 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    10021 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    10081 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    10141 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    10201 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    10261 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    10321 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    10381 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    10441 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    10501 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta
    10561 ggtgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta
    10621 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
    10681 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
    10741 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
    10801 acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca
    10861 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt
    10921 gattgcgcct gagcgagtcg aaatacgcga tcgctgttaa aaggacaatt acaaacagga
    10981 atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca
    11041 ggatattctt ctaatacctg gaatgctgtt ttccctggga tcgcagtggt gagtaaccat
    11101 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc
    11161 cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc
    11221 agaaacaact ctggcgcatc gggcttccca tacaatcggt agattgtcgc acctgattgc
    11281 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat
    11341 cgcggccttg agcaagacgt ttcccgttga atatggctca taacagaact tattatttcc
    11401 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc
    11461 caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt
    11521 gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc
    11581 acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg
    11641 tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa
    11701 gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta
    11761 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg
    11821 atatattgtg gtgtaaaca
//
