LOCUS       sequence-415042-       12916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12916)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1959
FEATURES             Location/Qualifiers
     source          1..12916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4113..4122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8773..9361
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8826..8846)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9244..9255)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9433..9666
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9744..9865
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9781..9800)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9835..9854
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9905..10040
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10060..10079)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10061..10079)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10089..10106)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10089..10105)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10098..10120)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10247..10702
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10334..10353)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10544..10565
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10728..10832
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10833..10901,10902..11693)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11051..11070)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11864..12452
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12353..12372
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12606..12623
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12740..12761
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12776..12781,12782..12799,12800..12806)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12814..12830
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12819..12841
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12838..12854
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12838..12854
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12873..12893
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12875..12893
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcg gccaatgtgg gatcgatgtt
     4141 tcaatattgg aagcgctttg atttacagca gctgcagaga gaactcgatg ccaccgcaac
     4201 ggtattggcg aaccggcagg atgaaagtga gcagtccaga aagcggctta tcgaacagag
     4261 ccgggagttc aagaagaaca ctccagagga tttgcgcaag caggtagcgc cgctgctgaa
     4321 gagtttccaa ggagagattg atgcactgag taaaagaagc aaggaagctg aagcagcttt
     4381 cttgaatgtc tacaaaagat tgattgacgt cccagatccc gtaccagctt tggatctcgg
     4441 acagcaactc cagctcaaag tgcagcgcct gcacgatatt gaaacagaga accagaaact
     4501 tagggaaact ctggaagaat acaacaagga atttgctgaa gtgaaaaatc aagaggttac
     4561 gataaaagca cttaaagaga aaatccgaga atatgaacag acactgaaga accaagccga
     4621 aaccatagct cttgagaagg aacagaagtt acagaatgac tttgcagaaa aggagagaaa
     4681 gctgcaggag acacagatgt ccaccacctc aaagctggag gaagctgagc ataaggttca
     4741 gagcctacaa acagccctgg aaaaaactcg aacagaatta tttgacctga aaaccaaata
     4801 cgatgaagaa actactgcaa aggccgacga gattgaaatg atcatgacgg accttgaaag
     4861 ggcaaaccag agggcagagg tggctcagag agaggcggag accttaaggg aacagctctc
     4921 atcggccaat cactccctcc agctggcctc acagatccag aaggcaccag acgtggagca
     4981 ggccatagag gtgctgaccc gctccagcct agaagttgag ttggccgcca aggagcggga
     5041 gatcgcacag ctggtggagg acgtgcagag actccaggcc agcctcacca agctgcggga
     5101 gaattcggcc agccagatct cacagcttga gcagcagctg agcgccaaaa acagcacact
     5161 caaacaactg gaagaaaaac tcaaaggcca ggctgactat gaagaggtga agaaagagct
     5221 gaacattctg aagtccatgg agtttgcacc gtccgagggc gctgggacac aggatgcggc
     5281 caagcccctg gaggtgctgt tgctggagaa gaaccgctcg ctgcagtccg agaacgccgc
     5341 gctgcgcatc tccaacagcg acctgagcgg gtcagccagg aggaaaggga aagaccagcc
     5401 tgaaagtcgg cgcccgggat ctttgccggc cccccctcct tctcagttgc cccgcaaccc
     5461 gggggagcag gcttccaata ctaatggtac acaccagttc tcaccagcgg ggttaagtca
     5521 agactttttc agctcatccc tggcaagccc cagcctaccc ctggcttcta caggaaaatt
     5581 tgcactaaac tctcttctcc agcggcagct aatgcagtcc ttctactcca aggctatgca
     5641 ggaagccgga agcacaagca tgattttttc aacaggtcca tacagcacaa actccatatc
     5701 ttcccaaagt ccattacaac aaagcccaga tgtcaatggc atggccccat cccccagcca
     5761 gtcagaaagt gctgggagcg tctccgaggg cgaggagatg gacactgcag aaatcgcccg
     5821 gcaggtcaaa gagcagctga ttaagcacaa tatcggacaa cgtattttcg gacattatgt
     5881 gttgggactg tctcaagggt ccgtgagcga gattctggcc cggcccaagc catggaataa
     5941 actgactgtt cgtggcaagg agccatttca caagatgaaa cagttcctct ccgatgagca
     6001 gaacatcctg gccctccgta gcatccaagg cagacaaaga gagaatccag gccagagcct
     6061 gaacagacta tttcaggaag taccgaaacg aagaaatggg tctgaaggta acatcaccac
     6121 ccggatccga gcctcggaga ctggctctga tgaagccatc aagtccatcc tagagcaagc
     6181 caagagggag ctccaagtgc agaaaactgc agagccggcc cagccttcct ccgcatccgg
     6241 cagcgggaac tctgatgacg ccatccgctc catcctgcag caagcccgcc gggagatgga
     6301 ggcccagcag gctgccctcg accctgcctt aaagcaggca ccactgtccc agagtgacat
     6361 caccatcctc acccccaagc ttctgtccac ctcgcccatg cccaccgtgt ccagctaccc
     6421 acctctcgcc atctccctga agaagccctc cgcagctcct gaggccggtg cctctgctct
     6481 gccgaacccc ccggccctca aaaaggaggc ccaggacgcc cccgggctgg acccccaggg
     6541 agcagccgat tgtgcacaag gggtcctgag acaggtgaaa aatgaggtgg gccgcagcgg
     6601 tgcctggaag gaccactggt ggagcgcggt gcagccggag agaagaaatg ccgcctcctc
     6661 cgaggaggcc aaggccgaag aaacgggcgg cgggaaagag aagggcagcg gtggcagcgg
     6721 aggtggcagc cagcctcggg ccgagcgcag tcagctccag ggaccctcgt cgtcagagta
     6781 ctggaaggag tggcccagcg ctgagtcccc atactcccag agctcagagc tgagtctgac
     6841 cggggccagc cgcagcgaga caccacagaa cagccccctg ccatcctccc cgatcgtgcc
     6901 catgtccaag cccaccaagc cctcggtccc cccgctgacc cccgagcagt acgaggtcta
     6961 catgtaccag gaggtggaca ccatcgagct cacccggcag gttaaggaaa agctggccaa
     7021 gaacggcatc tgccagagaa tcttcgggga gaaggtgctg ggcctgtccc agggcagcgt
     7081 cagcgacatg ctgtcccgac cgaagccatg gagcaagctg acgcagaaag gccgagaacc
     7141 cttcatccgg atgcagctct ggctgaacgg cgagctaggc cagggtgttc tacccgtcca
     7201 gggccagcag caagggccag tcctccactc cgtgacatcg ctccaggacc cgctgcagca
     7261 gggctgtgtg agctcagaaa gcactccaaa gacctccgcc agctgcagcc ctgcccctga
     7321 gtccccgatg agttccagtg agtcggtgaa gagcctgacc gagctggtcc agcagccctg
     7381 tccccccatc gaggcgagca aggacagcaa gccaccagag cccagtgacc cgccagcatc
     7441 cgactcccag cccacaaccc cgctgcctct ctccggacac tcggccctca gcatccaaga
     7501 attagtagcc atgtccccgg agctggacac ctacggcata accaagcggg tgaaggaggt
     7561 gctgacggac aacaacctcg gccagcgctt atttggggag accatcttag ggctcaccca
     7621 aggctctgtc tctgacctcc ttgcccgccc caaaccctgg cataagctca gtctgaaagg
     7681 acgagagccc ttcgtccgga tgcagctgtg gctgaacgac cccaacaatg tggagaagct
     7741 gatggacatg aaacggatgg agaagaaagc ctacatgaag cggcggcaca gctcagtcag
     7801 tgacagccag ccctgcgaac cgccctctgt cggcaccgag tacagccagg gcgccagccc
     7861 ccagccccag caccagctga agaaaccccg ggtggtgctg gctccggagg agaaggaggc
     7921 gctgaaacga gcgtatcagc aaaagccata cccgtcacca aaaaccatcg aagacctcgc
     7981 cacccagctc aacctgaaaa ccagcaccgt catcaactgg ttccacaact acaggtctcg
     8041 gatccgcaga gaactgttca ttgaggaaat tcaggccggg agtcagggcc aggcgggcgc
     8101 cagcgactca ccctcggccc gcagcggccg ggcggcgccc agctcggagg gcgacagctg
     8161 cgacggcgtg gaggccactg agggcccagg cagcgccgac accgaggagc ccaagtctca
     8221 gggagaggcc gagcgggagg aggtgccgcg gccggcggag cagacggagc cgccgccctc
     8281 ggggaccccg ggcccggacg acgcccgcga cgacgaccac gagggaggcc ccgtggaagg
     8341 cccggggccc ctgcccagcc ccgcctccgc gaccgccacc gccgcgcccg cggcccccga
     8401 ggacgccgct acctcagccg ccgccgcgcc gggggagggc cccgcggccc cgagctccgc
     8461 gccgccgccc agcaacagca gcagcagcag cgccccccgc aggcccagct cgctgcagag
     8521 ccttttcggc ctccccgagg ccgcgggcgc ccgggactcg cgcgacaacc ccctgcgcaa
     8581 gaagaaggcc gcgaacttga acagcatcat ccaccgcctg gagaaggccg ccagccggga
     8641 ggaacctatc gaatgggagt tctagtaatg aactagtccg taacttgaaa gtatttcgat
     8701 ttcttggctt tatatatctt gtggaaagga cgaaccggac caatcgtcgg taaggatacg
     8761 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     8821 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     8881 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     8941 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     9001 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     9061 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     9121 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     9181 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     9241 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     9301 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     9361 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     9421 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     9481 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     9541 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     9601 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     9661 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     9721 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     9781 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     9841 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     9901 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     9961 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    10021 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
    10081 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
    10141 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
    10201 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
    10261 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
    10321 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
    10381 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
    10441 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
    10501 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    10561 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
    10621 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
    10681 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
    10741 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
    10801 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
    10861 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
    10921 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
    10981 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
    11041 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
    11101 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
    11161 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
    11221 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
    11281 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
    11341 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
    11401 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
    11461 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
    11521 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
    11581 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    11641 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
    11701 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    11761 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    11821 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    11881 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    11941 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    12001 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    12061 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    12121 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    12181 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    12241 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    12301 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    12361 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    12421 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    12481 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    12541 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    12601 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    12661 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
    12721 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
    12781 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
    12841 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
    12901 agctggagct gcaagc
//
