LOCUS       sequence-41508-e         626 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Rishi V, Gal J, Krylov D, Fridriksson J, Boysen MS, Mandrup S, 
            Vinson C
  TITLE     SREBP-1 dimerization specificity maps to both the helix-loop-helix 
            and leucine zipper domains: use of a dominant negative.
  JOURNAL   J Biol Chem. 2004 Mar 19;279(12):11863-74. Epub 2003 Dec 31.
  PUBMED    14702347
REFERENCE   2  (bases 1 to 626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-03-19"; volume: "279(12)"; pages: "11863-74. 
            Epub 2003 Dec 31"
COMMENT     Sequence Label: CMV566 empty vector
FEATURES             Location/Qualifiers
     source          1..626
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             89..115
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(147..165)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(148..165)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(185..202)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    191..415
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(548..567)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctcca ccatggcgta tccctacgac gtgcccgatt atgcccatat
      121 gtgatgaagc ttctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga
      181 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
      241 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
      301 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
      361 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
      421 gaggcggaaa gaaccagctg gggctcgagg ggggatcccc acgcgccctg tagcggcgca
      481 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
      541 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
      601 caagctctaa atcgggggct cccttt
//
