LOCUS       sequence-41510-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Rishi V, Gal J, Krylov D, Fridriksson J, Boysen MS, Mandrup S, 
            Vinson C
  TITLE     SREBP-1 dimerization specificity maps to both the helix-loop-helix 
            and leucine zipper domains: use of a dominant negative.
  JOURNAL   J Biol Chem. 2004 Mar 19;279(12):11863-74. Epub 2003 Dec 31.
  PUBMED    14702347
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-03-19"; volume: "279(12)"; pages: "11863-74. 
            Epub 2003 Dec 31"
COMMENT     Sequence Label: CMV500 empty vector
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             89..112
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(144..162)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(145..162)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(182..199)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    188..412
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(545..564)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     755..776
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ccaccatgga ctacaaggac gacgatgaca agcatatgtg
      121 atgaagcttc tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca
      181 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
      241 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
      301 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
      361 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
      421 gcggaaagaa ccagctgggg ctcgaggggg gatccccacg cgccctgtag cggcgcatta
      481 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
      541 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
      601 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
      661 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
      721 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
      781 acactcaacc
//
