LOCUS       sequence-415143-       11839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Premade nuclei membrane marker, pUBQ10:H2B:mVenus:tHSP18.2M:FastRed,
            for braiding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11839)
  AUTHORS   Piepers M, Erbstein K, Reyes-Hernandez J, Song C, Tessi T, 
            Petrasiunaite V, Faerber N, Distel K, Maizel A
  TITLE     GreenGate 2.0: Backwards compatible addons for assembly of complex 
            transcriptional units and their stacking with GreenGate.
  JOURNAL   PLoS One. 2023 Sep 8;18(9):e0290097. doi: 
            10.1371/journal.pone.0290097. eCollection 2023.
  PUBMED    37682951
REFERENCE   2  (bases 1 to 11839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2023-09-8"; volume: "18(9):e0290097. doi"; pages: " 
            10.1371/journal.pone.0290097. eCollection 2023"
COMMENT     Sequence Label: pKD004
FEATURES             Location/Qualifiers
     source          1..11839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(277..984)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MLSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(552..571)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(join(3396..4109,4110..4112,4113..4115))
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3441..3462)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3790..3809
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4049..4070
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    5504..5528
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6829..7458
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7887..8960
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9026..9220
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9456..9478)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9564..9704
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9619..9638
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9719..9736)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9890..10478)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9970..9989)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10566..11381)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(10566..11381)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     11289..11308
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    11814..11838
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 taacgaattg ctcgagcacc tgccgatata ctttatccta gtttgcgcgc tatattttgt
       61 tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa
      121 ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata
      181 tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt
      241 ttgaacgatc tgcttgactc tagatactca catatgcttg tacagctcgt ccatgccgcc
      301 ggtggagtgg cggccctcgg cgcgttcgta ctgttccacg atggtgtagt cctcgttgtg
      361 ggaggtgatg tccaacttga tgttgacgtt gtaggcgccg ggcagctgca cgggcttctt
      421 ggccttgtag gtggtcttga cctcagcgtc gtagtggccg ccgtccttca gcttcagcct
      481 ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac atccgctcgg aggaggcctc
      541 ccagcccatg gtcttcttct gcattacggg gccgtcggag gggaagttgg tgccgcgcag
      601 cttcaccttg tagatgaact cgccgtcctg cagggaggag tcctgggtca cggtcaccac
      661 gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga aggacagctt
      721 caagtagtcg gggatgtcgg cggggtgctt cacgtaggcc ttggagccgt acatgaactg
      781 aggggacagg atgtcccagg cgaagggcag ggggccaccc ttggtcacct tcagcttggc
      841 ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc tcgatctcga actcgtggcc
      901 gttcacggag ccctccatgt gcaccttgaa gcgcatgaac tccttgatga tggccatgtt
      961 atcctcctcg cccttgctca acatagtagt gtgctggcca ccacgagtac ggtcacggtc
     1021 atgttcccca ccagtatgtt gctgtccgta gtactgagct ctgtctttca gatcctgagc
     1081 tttgcttccc aacttcatcc ttgcactgtc caacttgtct gatccctgtg ggtgctctcc
     1141 cgttgcgtac ctattcacaa tcaataggta tcataccaag cattttacct atatagatga
     1201 tatacacaga cacattcaag ctagctaggt caaatttcgt atttagttaa ccaaatgttc
     1261 cctagaattt gcaatttctt attgttacat ttgttattcg accaaaaaaa ttgatccaaa
     1321 gctactggct caacacatgc atgcacatat tgcacaaaat tatgaagtaa gatgataaat
     1381 gtgtgcttac ttgtaaatcc aagagaaaac ggttatagcg gcaatgccaa accctccaga
     1441 ggaaagaaaa ccggtgatga ggagtgcaac tgtgatgaga gccgggacaa ggattgggct
     1501 gaagataacg agcagaggtg ttgcaacagt caaagctatg acagttccaa caagggtaag
     1561 gctggagaga acaaggaggg aaccaccagc tgtgacagca gttgcagctt tagcaatctg
     1621 cctagacttg gagtagtcag atcctcgtcc ggacatctgg tactgatctc gatctcggcc
     1681 catcatcggg tactggtccc tgccgatgat atcgtgatgg gttcctctag ctgtatccgc
     1741 catttttttg ttcttgttta ctagagagaa tgagattttg aatgagagat tgatcaaaaa
     1801 gatgtgtata tattttggaa gaggtaggag tgtttgttag atatattaag aaaggagacg
     1861 agaagtcacg tgtcaatgga gacatcatgc aattgtaggc cacgtgttaa tggcgtcacc
     1921 atgcagccgc tgcatgtgtt aacgagagga gtctattcaa agacacgttt gttttttgcc
     1981 tagctcagaa tgtggcgttg gcatcgaacc gaaacataaa acgcaaaacg aacctttggg
     2041 ctcgtcagta tgggtttggg ccttcaatag attccttcta tacggcccac atatccacta
     2101 ttggcaacaa aattagaaaa atttaggatt tagttttaaa atttgttccg cgacattcta
     2161 gaacaatgaa gaaaatggat acaaataata aatctatctg cctcttaaaa gttccaaatt
     2221 tcaccacgtc actgctttga taatgcaggt cctcgtcgtc tctcctccgc tcatcgccgc
     2281 cgcatctctc tctaaacctc tcaattctct ctctaaagcc gcactctctt tctccagagc
     2341 caaacccatt tgtcctttcc ctcaaacctc tcgacgacca atctccgtat acaaatcccc
     2401 aatgaacaac cttttcaaca gactcggatt cgggtctggt cctcaagccc aagctgaccc
     2461 atcttccgcc gcaatcgccc aaggacccga cgatgatgtt ccgtcttcag gtcagcaatt
     2521 cgcgcaattt ggcgccggtt gtttctgggg agtggagcta gcttaccaga gagttcctgg
     2581 tgtgaccaag accgaggttg ggtatagcca tggcatcgtg cacaatccga gttacgagga
     2641 tgtctgtact ggcactacgg gacataacga ggttgttaga gttcagtatg atcccaaaga
     2701 gtgtagcttt gagagcttgc ttgatgtttt ctggaatcgt catgatccaa ccaccttgaa
     2761 tcgtcaggtg attgattgct ttaaccctaa aaatctagac gttatcagta tcagatcgta
     2821 atttgaatca atagagagct ctatgtgtgt tctgcatttg gggctgaatt gtttattaga
     2881 taacaacgac tagattcacc tgtttagacg aactctctgt cccttagtct ttagttctac
     2941 atacatttgg tagtctcaag taactattgg ctaagttgtt atgagctagt tagaatctca
     3001 ggccttgttg atatgaggat tcgtgtttga ttatgtggaa gtgacattgc catttccatt
     3061 ttcgtatgcg atcttgtact tgagtctgtt cttgttcatg ttactatacc tacatacact
     3121 tgaagtagtg aattccttat ctttaatcat attccatagt ccataccata gcacatacag
     3181 tagttatatg ctgcagaaga gatccaacaa aacattcaca atggattata gaaacatttg
     3241 tttattcatt ataatgagat cttacattca tttaatatta gaaaaagcca caaattcata
     3301 acacaacaag ccaagaaaaa aacacaaact taagcacaca agctatttat ttgacacacc
     3361 aaatatttca tcttcatctt catatgagct cgcagctact tgtacagctc gtccatgccg
     3421 agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg
     3481 gggtctttgc tcagcttgga ctggtagctc aggtagtggt tgtcgggcag cagcacgggg
     3541 ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgcc gccgtcctcg
     3601 atgttgtggc ggatcttgaa gttggccttg atgccgttct tctgcttgtc ggcggtgata
     3661 tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc
     3721 ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc
     3781 tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag
     3841 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcgggcg
     3901 aagcactgca ggccgtagcc cagggtggtc acgagggtgg gccagggcac gggcagcttg
     3961 ccggtggtgc agatcagctt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
     4021 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
     4081 accccggtga acagctcctc gcccttgctc accatggatc cgcctccacc actagtgcca
     4141 ccgctgccac cgctagcacc tgaacttgaa gagccggaac cagatccgga ccctgaaccc
     4201 gaacctgatc ctgatccgga cccactacct gagccagatc cggagccaga acctgaagca
     4261 gcagcggagc ctgcagcggc cgccgatcca gagccgctgc cggaaccact ccctgagccc
     4321 gatccagagc cgcttccgga accactccct gatgcagacg cactagcggc tgtcgaacct
     4381 gaagagctag taaacttagt aacagcctta gttccttcag aaacagcatg cttagcaagt
     4441 tcaccaggta acactagtct aacagctgtc tgaatctccc tagaagtaat cgtaggtttc
     4501 ttattatacc tagctaattt tgaagcttct tgcgcaagtt tctcgaatat atcattgata
     4561 aaactgttca taatccccat agctttactt gagattccaa tatctggatg aacttgtttc
     4621 aacaccttga agatgtagat cttgtatgtc tctgtgcttt tcttgactcg tttcttcttc
     4681 ttctcgactc ctccggtgac tgcttctttt ggtagttttt ttccggcttt tggtttcttc
     4741 tctgctggtg ctttttcggc tactttctct tcttctgctg gagctttctc tgcgggcttt
     4801 ttctctgctg gtttctttcc ggcttttggt gccattgttt gttaatcaga aaaactcaga
     4861 ttaatctaca aattcgatcg cacaaactag aaactaacac cagatctaga tagaaatcac
     4921 aaatcgaaga gtaattattc gacaaaactc aaattatttg aacaaatcgg atgatatcta
     4981 tgaaacccta atcgagaatt aagatgatat ctaacgatca aacccagaaa atcgtcttcg
     5041 atctaagatt aacagaatct aaaccaaaga acatatacga aattgggatc gaacgaaaac
     5101 aaaatcgaag attttgagag aataaggaac acagaaattt accttgatca cggtagagag
     5161 aattgagaga aagtttttaa gattttgaga aattgaaatc tgaattgtga agaagaagag
     5221 ctctttgggt attgttttat agaagaagaa gaagaaaaga cgaggacgac taggtcacga
     5281 gaaagctaag gcggtgaagc aatagctaat aataaaatga cacgtgtatt gagcgttgtt
     5341 tacacgcaaa gttgtttttg gctaattgcc ttatttttag gttgaggaaa agtatttgtg
     5401 ctttgagttg ataaacacga ctcgtgtgtg ccggctgcaa ccactttgac gccgtttatt
     5461 actgactcgt cgaggttgac tgtagcaggt gccatggtaa ttctgacagg atatattggc
     5521 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
     5581 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
     5641 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
     5701 agccgtcatc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
     5761 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
     5821 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
     5881 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
     5941 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
     6001 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
     6061 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
     6121 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
     6181 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
     6241 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     6301 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     6361 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     6421 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     6481 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     6541 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     6601 cgggtacgtg ttcgagccgc ccgcgcacct ctcaaccgtg cggctgcatg aaatcctggc
     6661 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     6721 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     6781 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     6841 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     6901 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     6961 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     7021 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     7081 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     7141 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     7201 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     7261 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     7321 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     7381 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     7441 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     7501 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     7561 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     7621 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
     7681 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     7741 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     7801 ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact
     7861 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
     7921 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
     7981 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
     8041 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
     8101 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
     8161 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
     8221 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
     8281 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
     8341 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
     8401 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
     8461 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
     8521 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
     8581 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
     8641 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
     8701 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
     8761 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
     8821 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
     8881 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
     8941 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
     9001 aaaaaggtcg aaaaggactc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
     9061 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
     9121 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
     9181 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
     9241 ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
     9301 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
     9361 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
     9421 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggtg
     9481 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     9541 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     9601 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     9661 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     9721 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     9781 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     9841 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     9901 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     9961 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    10021 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    10081 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    10141 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    10201 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    10261 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    10321 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    10381 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    10441 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    10501 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta
    10561 ggtgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta
    10621 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
    10681 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
    10741 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
    10801 acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca
    10861 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt
    10921 gattgcgcct gagcgagtcg aaatacgcga tcgctgttaa aaggacaatt acaaacagga
    10981 atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca
    11041 ggatattctt ctaatacctg gaatgctgtt ttccctggga tcgcagtggt gagtaaccat
    11101 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc
    11161 cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc
    11221 agaaacaact ctggcgcatc gggcttccca tacaatcggt agattgtcgc acctgattgc
    11281 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat
    11341 cgcggccttg agcaagacgt ttcccgttga atatggctca taacagaact tattatttcc
    11401 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc
    11461 caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt
    11521 gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc
    11581 acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg
    11641 tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa
    11701 gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta
    11761 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg
    11821 atatattgtg gtgtaaaca
//
