LOCUS       sequence-415145-       11818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Premade nuclei membrane marker, 
            pUBQ10:H2B:PmScarlet-I:tHSP18.2M:FastRed, for braiding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11818)
  AUTHORS   Piepers M, Erbstein K, Reyes-Hernandez J, Song C, Tessi T, 
            Petrasiunaite V, Faerber N, Distel K, Maizel A
  TITLE     GreenGate 2.0: Backwards compatible addons for assembly of complex 
            transcriptional units and their stacking with GreenGate.
  JOURNAL   PLoS One. 2023 Sep 8;18(9):e0290097. doi: 
            10.1371/journal.pone.0290097. eCollection 2023.
  PUBMED    37682951
REFERENCE   2  (bases 1 to 11818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2023-09-8"; volume: "18(9):e0290097. doi"; pages: " 
            10.1371/journal.pone.0290097. eCollection 2023"
COMMENT     Sequence Label: pKD001
FEATURES             Location/Qualifiers
     source          1..11818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4499..5206
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MLSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     4912..4931
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5483..5507
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6808..7437
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7866..8939
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9005..9199
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9435..9457)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9543..9683
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9598..9617
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9698..9715)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9869..10457)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9949..9968)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10545..11360)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(10545..11360)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     11268..11287
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    11793..11817
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 taacgaatta ccatggcacc tgctgcaacc tcgacgagtc agtaataaac ggcgtcaaag
       61 tggttgcagc cggcacacac gagtcgtgtt tatcaactca aagcacaaat acttttcctc
      121 aacctaaaaa taaggcaatt agccaaaaac aactttgcgt gtaaacaacg ctcaatacac
      181 gtgtcatttt attattagct attgcttcac cgccttagct ttctcgtgac ctagtcgtcc
      241 tcgtcttttc ttcttcttct tctataaaac aatacccaaa gagctcttct tcttcacaat
      301 tcagatttca atttctcaaa atcttaaaaa ctttctctca attctctcta ccgtgatcaa
      361 ggtaaatttc tgtgttcctt attctctcaa aatcttcgat tttgttttcg ttcgatccca
      421 atttcgtata tgttctttgg tttagattct gttaatctta gatcgaagac gattttctgg
      481 gtttgatcgt tagatatcat cttaattctc gattagggtt tcatagatat catccgattt
      541 gttcaaataa tttgagtttt gtcgaataat tactcttcga tttgtgattt ctatctagat
      601 ctggtgttag tttctagttt gtgcgatcga atttgtagat taatctgagt ttttctgatt
      661 aacaaacaat ggcaccaaaa gccggaaaga aaccagcaga gaaaaagccc gcagagaaag
      721 ctccagcaga agaagagaaa gtagccgaaa aagcaccagc agagaagaaa ccaaaagccg
      781 gaaaaaaact accaaaagaa gcagtcaccg gaggagtcga gaagaagaag aaacgagtca
      841 agaaaagcac agagacatac aagatctaca tcttcaaggt gttgaaacaa gttcatccag
      901 atattggaat ctcaagtaaa gctatgggga ttatgaacag ttttatcaat gatatattcg
      961 agaaacttgc gcaagaagct tcaaaattag ctaggtataa taagaaacct acgattactt
     1021 ctagggagat tcagacagct gttagactag tgttacctgg tgaacttgct aagcatgctg
     1081 tttctgaagg aactaaggct gttactaagt ttactagctc ttcaggttcg acagccgcta
     1141 gtgcgtctgc atcagggagt ggttccggaa gcggctctgg atcgggctca gggagtggtt
     1201 ccggcagcgg ctctggatcg gcggccgctg caggctccgc tgctgcttca ggttctggct
     1261 ccggatctgg ctcaggtagt gggtccggat caggatcagg ttcgggttca gggtccggat
     1321 ctggttccgg ctcttcaagt tcaggtgcta gcggtggcag cggtggcact agtggtggag
     1381 gcggatccat ggtgagcaag ggcgaggcag tgatcaaaga gttcatgagg ttcaaggtcc
     1441 acatggaagg cagcatgaac ggacatgagt tcgagatcga aggtgaaggt gagggtagac
     1501 cttacgaggg aactcagact gctaagctca aggtgacaaa aggtggaccg cttccgttca
     1561 gctgggatat tctttcaccg cagttcatgt acggcagcag ggctttcatc aagcaccctg
     1621 ctgatattcc ggactactac aagcagtctt tcccagaggg attcaagtgg gagcgtgtga
     1681 tgaatttcga ggatggtggt gctgtgactg tgacccagga tacttctctt gaggatggga
     1741 ccctcatcta caaggtgaag ctcaggggaa ctaacttccc tcctgatggt cctgtgatgc
     1801 agaaaaagac tatgggctgg gaagcctcta ccgagagact ttatcctgag gacggtgtgc
     1861 ttaagggcga catcaagatg gctcttaggc ttaaggacgg tggacgttac ctcgctgatt
     1921 tcaagactac ctacaaggcc aagaaaccgg tgcaaatgcc tggggcttac aacgttgaca
     1981 gaaagctcga catcaccagc cacaacgagg attacactgt ggttgagcag tacgagaggt
     2041 ctgagggtcg tcattctact ggtggcatgg acgagctgta caagtagctg cgagctcata
     2101 tgaagatgaa gatgaaatat ttggtgtgtc aaataaatag cttgtgtgct taagtttgtg
     2161 tttttttctt ggcttgttgt gttatgaatt tgtggctttt tctaatatta aatgaatgta
     2221 agatctcatt ataatgaata aacaaatgtt tctataatcc attgtgaatg ttttgttgga
     2281 tctcttctgc agcatataac tactgtatgt gctatggtat ggactatgga atatgattaa
     2341 agataaggaa ttcactactt caagtgtatg taggtatagt aacatgaaca agaacagact
     2401 caagtacaag atcgcatacg aaaatggaaa tggcaatgtc acttccacat aatcaaacac
     2461 gaatcctcat atcaacaagg cctgagattc taactagctc ataacaactt agccaatagt
     2521 tacttgagac taccaaatgt atgtagaact aaagactaag ggacagagag ttcgtctaaa
     2581 caggtgaatc tagtcgttgt tatctaataa acaattcagc cccaaatgca gaacacacat
     2641 agagctctct attgattcaa attacgatct gatactgata acgtctagat ttttagggtt
     2701 aaagcaatca atcacctgac gattcaaggt ggttggatca tgacgattcc agaaaacatc
     2761 aagcaagctc tcaaagctac actctttggg atcatactga actctaacaa cctcgttatg
     2821 tcccgtagtg ccagtacaga catcctcgta actcggattg tgcacgatgc catggctata
     2881 cccaacctcg gtcttggtca caccaggaac tctctggtaa gctagctcca ctccccagaa
     2941 acaaccggcg ccaaattgcg cgaattgctg acctgaagac ggaacatcat cgtcgggtcc
     3001 ttgggcgatt gcggcggaag atgggtcagc ttgggcttga ggaccagacc cgaatccgag
     3061 tctgttgaaa aggttgttca ttggggattt gtatacggag attggtcgtc gagaggtttg
     3121 agggaaagga caaatgggtt tggctctgga gaaagagagt gcggctttag agagagaatt
     3181 gagaggttta gagagagatg cggcggcgat gagcggagga gagacgacga ggacctgcat
     3241 tatcaaagca gtgacgtggt gaaatttgga acttttaaga ggcagataga tttattattt
     3301 gtatccattt tcttcattgt tctagaatgt cgcggaacaa attttaaaac taaatcctaa
     3361 atttttctaa ttttgttgcc aatagtggat atgtgggccg tatagaagga atctattgaa
     3421 ggcccaaacc catactgacg agcccaaagg ttcgttttgc gttttatgtt tcggttcgat
     3481 gccaacgcca cattctgagc taggcaaaaa acaaacgtgt ctttgaatag actcctctcg
     3541 ttaacacatg cagcggctgc atggtgacgc cattaacacg tggcctacaa ttgcatgatg
     3601 tctccattga cacgtgactt ctcgtctcct ttcttaatat atctaacaaa cactcctacc
     3661 tcttccaaaa tatatacaca tctttttgat caatctctca ttcaaaatct cattctctct
     3721 agtaaacaag aacaaaaaaa tggcggatac agctagagga acccatcacg atatcatcgg
     3781 cagggaccag tacccgatga tgggccgaga tcgagatcag taccagatgt ccggacgagg
     3841 atctgactac tccaagtcta ggcagattgc taaagctgca actgctgtca cagctggtgg
     3901 ttccctcctt gttctctcca gccttaccct tgttggaact gtcatagctt tgactgttgc
     3961 aacacctctg ctcgttatct tcagcccaat ccttgtcccg gctctcatca cagttgcact
     4021 cctcatcacc ggttttcttt cctctggagg gtttggcatt gccgctataa ccgttttctc
     4081 ttggatttac aagtaagcac acatttatca tcttacttca taattttgtg caatatgtgc
     4141 atgcatgtgt tgagccagta gctttggatc aatttttttg gtcgaataac aaatgtaaca
     4201 ataagaaatt gcaaattcta gggaacattt ggttaactaa atacgaaatt tgacctagct
     4261 agcttgaatg tgtctgtgta tatcatctat ataggtaaaa tgcttggtat gatacctatt
     4321 gattgtgaat aggtacgcaa cgggagagca cccacaggga tcagacaagt tggacagtgc
     4381 aaggatgaag ttgggaagca aagctcagga tctgaaagac agagctcagt actacggaca
     4441 gcaacatact ggtggggaac atgaccgtga ccgtactcgt ggtggccagc acactactat
     4501 gttgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     4561 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     4621 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     4681 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     4741 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     4801 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     4861 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     4921 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     4981 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     5041 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     5101 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     5161 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagcata tgtgagtatc
     5221 tagagtcaag cagatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     5281 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     5341 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     5401 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa agtatgtcat
     5461 atggcaggtg ctcgagcaat tctgacagga tatattggcg ggtaaaccta agagaaaaga
     5521 gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
     5581 ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc
     5641 tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcatct gaaaacgaca
     5701 tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc
     5761 ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg
     5821 ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc
     5881 aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg
     5941 agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac
     6001 gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc
     6061 tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc
     6121 cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg
     6181 ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg
     6241 cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc
     6301 gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc
     6361 atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca
     6421 ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg
     6481 agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt
     6541 tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc
     6601 cgcgcacctc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct
     6661 ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg
     6721 atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt
     6781 aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc
     6841 gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc
     6901 gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg
     6961 gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag
     7021 gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct
     7081 gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac
     7141 atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga
     7201 aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag
     7261 gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc
     7321 gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc
     7381 gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt
     7441 aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg
     7501 cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg
     7561 tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag
     7621 gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca
     7681 aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa
     7741 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc
     7801 gtaagcggct gggttgcctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa
     7861 tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg
     7921 acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag
     7981 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac
     8041 cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt
     8101 ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg
     8161 ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc
     8221 cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg
     8281 acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga
     8341 agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc
     8401 ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca
     8461 ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat
     8521 ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg
     8581 agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc
     8641 cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc
     8701 tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga
     8761 tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc
     8821 tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc
     8881 cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat
     8941 gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggactct
     9001 ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt
     9061 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa
     9121 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa
     9181 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc
     9241 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg
     9301 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg
     9361 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt
     9421 gatgacggtg aaaacctctg acacatgcag ctcccggtga cggtcacagc ttgtctgtaa
     9481 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     9541 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     9601 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     9661 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     9721 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     9781 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     9841 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     9901 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     9961 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    10021 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    10081 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    10141 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    10201 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    10261 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
    10321 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    10381 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    10441 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    10501 acgaaaactc acgttaaggg attttggtca tgcattctag gtgattagaa aaactcatcg
    10561 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
    10621 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
    10681 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
    10741 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
    10801 ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
    10861 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagtcga
    10921 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
    10981 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
    11041 aatgctgttt tccctgggat cgcagtggtg agtaaccatg catcatcagg agtacggata
    11101 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
    11161 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
    11221 ggcttcccat acaatcggta gattgtcgca cctgattgcc cgacattatc gcgagcccat
    11281 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggccttga gcaagacgtt
    11341 tcccgttgaa tatggctcat aacagaactt attatttcct tcctcttttc tacagtattt
    11401 aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt
    11461 ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa
    11521 catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca
    11581 tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta
    11641 gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct
    11701 ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg
    11761 agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaaca
//
