LOCUS       sequence-415149-       12999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses BcLOV-SOS for optogenetic activation of Ras/Erk signaling.
            Includes an mCherry tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12999)
  AUTHORS   Pal AA, Benman W, Mumford TR, Huang Z, Chow BY, Bugaj LJ
  TITLE     Optogenetic clustering and membrane translocation of the BcLOV4 
            photoreceptor.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Aug 8;120(32):e2221615120. doi: 
            10.1073/pnas.2221615120. Epub 2023 Aug 1.
  PUBMED    37527339
REFERENCE   2  (bases 1 to 12999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-08-8"; volume: "120(32):e2221615120. 
            doi"; pages: " 10.1073/pnas.2221615120. Epub 2023 Aug 1"
COMMENT     Sequence Label: BcLOV-mCh-SOS
FEATURES             Location/Qualifiers
     source          1..12999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(41..61)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        46..375
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      226..361
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1509..1574
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1704..1724
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2149..2283
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2186..2205)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2240..2259
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2451..3084
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3131..3256
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3753..3986
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4171..4215
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4500..4617
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4770..5177
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             7023..7730
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7168..7186)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7436..7455
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    9313..9901
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9366..9386)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9784..9795)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9904..9920)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9905..9921)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10011..10244
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(10269..10287)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10270..10287)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10577..10596)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10810..10829)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10929..10951
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10989..11007)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11075..11179
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11180..11248,11249..12040)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11398..11417)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12211..12799
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12700..12719
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12953..12970
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg
       61 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
      121 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
      181 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
      241 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
      301 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
      361 ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc
      421 actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact
      481 ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt
      541 ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg
      601 ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg
      661 gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa
      721 ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc
      781 gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac
      841 ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt
      901 gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc
      961 ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc
     1021 tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg
     1081 cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct
     1141 gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg
     1201 ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca
     1261 ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat
     1321 cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata
     1381 cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg
     1441 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa
     1501 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
     1561 tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct
     1621 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg
     1681 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact
     1741 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt
     1801 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa
     1861 aatattctgt aacctttata agtaggcata acagttataa tcataacata ctgttttttc
     1921 ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct
     1981 ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta
     2041 gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     2101 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     2161 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     2221 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     2281 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt
     2341 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt
     2401 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct
     2461 aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     2521 cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
     2581 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
     2641 gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     2701 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
     2761 ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt
     2821 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata
     2881 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     2941 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     3001 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     3061 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     3121 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     3181 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     3241 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     3301 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     3361 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     3421 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     3481 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     3541 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg
     3601 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     3661 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     3721 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     3781 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     3841 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     3901 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     3961 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     4021 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     4081 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     4141 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     4201 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     4261 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     4321 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     4381 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     4441 tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt catccacaat
     4501 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4561 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     4621 gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat cgaattcctg
     4681 cagccccgat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
     4741 ccacctgtag gtttggcaag ctagctgcag taacgccatt ttgcaaggca tggaaaaata
     4801 ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag ctaacgttgg
     4861 gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa gaacagatgg
     4921 tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga tatggcccaa
     4981 ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg acctgaaatg
     5041 accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt cgcgcgcttc
     5101 tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca gtcctccgac
     5161 agactgagtc gcccgggggg gatctggagc tctcgagaat tctcacgcgt atggccacag
     5221 acgcaatcga ccattactac cagaacagag acggcccccc ttccaggagc tttagcgacg
     5281 aatacgactc atacaataag acagcccctc tgccacctct gagcgcctgc ccagagcgcg
     5341 cccagacccc taacagcaag agaatcccca tcacacccaa ttccgccaag agcacctccg
     5401 agtctcctcg gagaatcaca ttctctcgcg acgcaggcag ctccaccaag ggcagcaccc
     5461 agacaagccc atccaaccag ggctccgtgt ctagccggaa tggctctaac ggaaatggcc
     5521 tgggcctgtc ctctgccggc aacttcgccg atttctttag ctccgaggtg ttttatatcg
     5581 tgctgcacaa tcccaccaca gcccacagat tcctgaagtt ttgccagagc agggcctgtt
     5641 ccgagaacct ggagtttctg cagaagatcg acgagtacaa taggctgatc gatgaggcca
     5701 cacgcctgct gtctaccatc tacagcacat acgtgagcag cgaggcccct aagcagatca
     5761 acatctctaa tagccacgcc aggcgcctga gccacgacat caagggagca acccacaacg
     5821 tgctgcccgg cctggaggat atcttcaatg gctcccagga gcagatcgag aagctgctgg
     5881 cctctgacct gtatcctaga tttgtgaagc accaggtgac cgcatccgcc acaatggccc
     5941 tggcaaacga caaggagagg tacccaggcc tgggcgattg cttctgtctg acagacccac
     6001 ggagagccga taatcccatc gtgttcgcaa gcgacggatt cgtgagcgtg accggctatt
     6061 cccgctctga tatcatccca aggaactgcc gcttcctgca gggcagcttt accgaccggc
     6121 aggccacaaa gaggctgcgc acctccatcg agaatcgcga ggagacagtg gagctgctgc
     6181 tgaactatcg gaagaatggc gatccctttt ggaacctgct gtacgtgtcc cctctgctgg
     6241 acggcaatgg cgatgtgaga ttctttctgg gcggccagat caactgttct accacaatcc
     6301 acagccggac cgacgtgctg agaatcctga gcctgaacga cgaggataca ggctccttca
     6361 tcgagggctc taataagacc cccagcgtgc ggtccaagga gtctaacaat gcctccaact
     6421 atggcaagac atccttcttt aagtctttta agaagtacaa ggccaatacc gtggagatca
     6481 gagacgaggc cggcatggag ggcgagctga tcgataggat cggcaagctg cagttcaaga
     6541 cacaggtgga ggagttttac accgcctatt ctaagtacct ggtgctgagc tatcagccac
     6601 agtcccagaa cctgatcgtg aagtacttca gccccggcat cgtggatatg ctgaacctga
     6661 atatgcctaa tggcctggtg gccccaatcg tggacaagga tatcttcaag gtgctgacag
     6721 agcactcccc ttcctctgtg ccacggacct ttaagaacgt ggtgagggaa ggcatcaaag
     6781 caggcagggc cgtgtctgtg gagacaggcc tgctgacagg catcgaggag atcaagaagt
     6841 ctcagaacat cttcagcggc accacagcca atcgggagag aggctggaag aaggccgagg
     6901 agaagtacgt gtgccacttt accccctgta aggatgagat gggcagggtc ggctgggtgg
     6961 tgctgactgt cgctcctaaa ctggaaagat ttgaattcgg tggtggatca ggtggaggat
     7021 ctatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     7081 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     7141 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     7201 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     7261 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     7321 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     7381 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     7441 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     7501 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     7561 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     7621 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     7681 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag ggtggtggat
     7741 caggtggagg atctcaacca ctgagattac caagtcctga agtatatcgt tttgtagtaa
     7801 aagactctga ggaaaacatt gtttttgaag acaacttgca aagtagaagt ggcatcccca
     7861 ttattaaagg aggaactgta gtgaaattaa ttgaaaggtt aacatatcat atgtatgcag
     7921 atcccaattt tgttcgtact tttcttacca catatcgttc attttgtaaa ccacaggaat
     7981 tgctgagctt actgattgaa cggtttgaaa ttccagagcc agaacctact gacgcagaca
     8041 aattggcaat agagaaaggc gagcagccaa tcagtgcaga ccttaaaaga tttcgcaagg
     8101 aatatgtcca accagtacaa cttaggatct taaatgtatt tcggcattgg gttgaacatc
     8161 atttttatga ctttgaaaga gacttggaat tgcttgaaag actagaatcc ttcatttcaa
     8221 gtgtaagagg gaaagctatg aaaaaatggg tagagtcaat tgctaagatc atcaggagga
     8281 agaagcaagc tcaggcaaac ggagtaagcc ataatattac ctttgaaagt ccacctccac
     8341 caattgaatg gcatatcagc aaaccaggac agtttgaaac atttgatctc atgacacttc
     8401 atccaataga aattgcacgt cagctgacac ttttggagtc tgatctttac aggaaagttc
     8461 aaccgtctga acttgtaggg agtgtgtgga ccaaagaaga taaagaaata aattctccaa
     8521 atttattaaa aatgattcgc cataccacaa atctcaccct ctggtttgaa aaatgcattg
     8581 tggaagcaga aaattttgaa gaacgggtgg cagtactaag tagaattata gaaattctgc
     8641 aagtttttca agatttgaat aatttcaatg gcgtattgga gatagtcagt gcagtaaatt
     8701 cagtgtcagt atacagacta gaccatacct ttgaggcact gcaggaaagg aaaaggaaaa
     8761 ttttggacga agctgtggaa ttaagtcaag atcactttaa aaaataccta gtaaaactta
     8821 agtcaatcaa tccaccttgt gtgccttttt ttggaatata tttaacaaat attctgaaga
     8881 ccgaagaagg gaataatgat tttttaaaaa agaaagggaa agatttaatc aatttcagta
     8941 agaggaggaa agtagctgaa attactggag aaattcagca gtatcagaat cagccttact
     9001 gtttacggat agaaccagat atgaggagat tctttgaaaa ccttaacccc atgggaagtg
     9061 catctgaaaa agagtttaca gattatttgt tcaacaagtc actagaaatt gaacctcgaa
     9121 actgcaaaca gccacctcga tttcctagga aatcaacttt ttccttaaaa tctcctggaa
     9181 taaggcctaa cacaggccga catggctcta cctaagcggc cgcaactccc acctgcaaca
     9241 tgcgtgactg actgaggccg cgactctaga gtcgacctgc aggcatgcaa gcttgatatc
     9301 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     9361 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     9421 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     9481 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     9541 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     9601 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     9661 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     9721 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     9781 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     9841 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     9901 catcgatacc gtcgacctcg agggaattaa tttgagctcg gtacctttaa gaccaatgac
     9961 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
    10021 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
    10081 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
    10141 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
    10201 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt
    10261 ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa
    10321 ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg
    10381 aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc
    10441 ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg
    10501 gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca
    10561 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac
    10621 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga
    10681 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
    10741 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg
    10801 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
    10861 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
    10921 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
    10981 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
    11041 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
    11101 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
    11161 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
    11221 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
    11281 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
    11341 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
    11401 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
    11461 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
    11521 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
    11581 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
    11641 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
    11701 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
    11761 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
    11821 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
    11881 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
    11941 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
    12001 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
    12061 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
    12121 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    12181 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    12241 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    12301 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
    12361 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    12421 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    12481 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    12541 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    12601 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    12661 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    12721 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    12781 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    12841 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    12901 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
    12961 agtgagcgag gaagcggaag agcgcccaat acgcaaacc
//
