LOCUS       sequence-415152-       13252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses BcLOV-EGFR for optogenetic activation of EGFR signaling. 
            Includes an mCherry tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13252)
  AUTHORS   Pal AA, Benman W, Mumford TR, Huang Z, Chow BY, Bugaj LJ
  TITLE     Optogenetic clustering and membrane translocation of the BcLOV4 
            photoreceptor.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Aug 8;120(32):e2221615120. doi: 
            10.1073/pnas.2221615120. Epub 2023 Aug 1.
  PUBMED    37527339
REFERENCE   2  (bases 1 to 13252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-08-8"; volume: "120(32):e2221615120. 
            doi"; pages: " 10.1073/pnas.2221615120. Epub 2023 Aug 1"
COMMENT     Sequence Label: BcLOV-mCh-EGFR
FEATURES             Location/Qualifiers
     source          1..13252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1236..1301
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1431..1451
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1876..2010
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1913..1932)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1967..1986
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2178..2811
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2858..2983
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3480..3713
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3898..3942
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4227..4344
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4425..4728
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4729..4932
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4883..4903
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      4985..4994
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6803..7510
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6948..6966)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7216..7235
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    9293..9881
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9346..9366)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9764..9775)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9884..9900)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9885..9901)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9991..10224
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(10249..10267)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10250..10267)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10557..10576)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10790..10809)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10909..10931
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10969..10987)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11055..11159
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11160..11228,11229..12020)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      12191..12779
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12680..12699
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12933..12950
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(13020..13040)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(13025..13252,1..102)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(13205..13252,1..88)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
       61 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg acacaagaca
      121 ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag gcagtgcgta
      181 aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc
      241 gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca
      301 catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc
      361 aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct
      421 gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt
      481 tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat
      541 ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc ggttgcgatt
      601 cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg
      661 ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca gccgtgggat
      721 atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcactg
      781 ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa gtattctgga
      841 ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca
      901 tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc
      961 ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat
     1021 ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg tgatgagcga
     1081 tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg gcggcaactg
     1141 gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt gtgacataat
     1201 tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat
     1261 aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg
     1321 atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa
     1381 tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga
     1441 agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg
     1501 ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg
     1561 cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca
     1621 gttataatca taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta
     1681 ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg
     1741 aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag
     1801 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     1861 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     1921 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     1981 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
     2041 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
     2101 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
     2161 actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga
     2221 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
     2281 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
     2341 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
     2401 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
     2461 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
     2521 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
     2581 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
     2641 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     2701 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     2761 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     2821 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     2881 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     2941 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     3001 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     3061 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     3121 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     3181 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     3241 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     3301 taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg
     3361 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     3421 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     3481 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     3541 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     3601 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     3661 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     3721 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     3781 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     3841 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     3901 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     3961 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     4021 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     4081 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     4141 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     4201 cgccaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     4261 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     4321 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     4381 atcgataagc ttgatatcga attcagccca tatatggagt tccgcgttac ataacttacg
     4441 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     4501 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     4561 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     4621 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     4681 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     4741 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     4801 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     4861 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     4921 ataagcagag ctggtttagt gaaccgtcag atccgctagc gctacgcgtc ccaagctgga
     4981 attcgccacc atggccacag acgcaatcga ccattactac cagaacagag acggcccccc
     5041 ttccaggagc tttagcgacg aatacgactc atacaataag acagcccctc tgccacctct
     5101 gagcgcctgc ccagagcgcg cccagacccc taacagcaag agaatcccca tcacacccaa
     5161 ttccgccaag agcacctccg agtctcctcg gagaatcaca ttctctcgcg acgcaggcag
     5221 ctccaccaag ggcagcaccc agacaagccc atccaaccag ggctccgtgt ctagccggaa
     5281 tggctctaac ggaaatggcc tgggcctgtc ctctgccggc aacttcgccg atttctttag
     5341 ctccgaggtg ttttatatcg tgctgcacaa tcccaccaca gcccacagat tcctgaagtt
     5401 ttgccagagc agggcctgtt ccgagaacct ggagtttctg cagaagatcg acgagtacaa
     5461 taggctgatc gatgaggcca cacgcctgct gtctaccatc tacagcacat acgtgagcag
     5521 cgaggcccct aagcagatca acatctctaa tagccacgcc aggcgcctga gccacgacat
     5581 caagggagca acccacaacg tgctgcccgg cctggaggat atcttcaatg gctcccagga
     5641 gcagatcgag aagctgctgg cctctgacct gtatcctaga tttgtgaagc accaggtgac
     5701 cgcatccgcc acaatggccc tggcaaacga caaggagagg tacccaggcc tgggcgattg
     5761 cttctgtctg acagacccac ggagagccga taatcccatc gtgttcgcaa gcgacggatt
     5821 cgtgagcgtg accggctatt cccgctctga tatcatccca aggaactgcc gcttcctgca
     5881 gggcagcttt accgaccggc aggccacaaa gaggctgcgc acctccatcg agaatcgcga
     5941 ggagacagtg gagctgctgc tgaactatcg gaagaatggc gatccctttt ggaacctgct
     6001 gtacgtgtcc cctctgctgg acggcaatgg cgatgtgaga ttctttctgg gcggccagat
     6061 caactgttct accacaatcc acagccggac cgacgtgctg agaatcctga gcctgaacga
     6121 cgaggataca ggctccttca tcgagggctc taataagacc cccagcgtgc ggtccaagga
     6181 gtctaacaat gcctccaact atggcaagac atccttcttt aagtctttta agaagtacaa
     6241 ggccaatacc gtggagatca gagacgaggc cggcatggag ggcgagctga tcgataggat
     6301 cggcaagctg cagttcaaga cacaggtgga ggagttttac accgcctatt ctaagtacct
     6361 ggtgctgagc tatcagccac agtcccagaa cctgatcgtg aagtacttca gccccggcat
     6421 cgtggatatg ctgaacctga atatgcctaa tggcctggtg gccccaatcg tggacaagga
     6481 tatcttcaag gtgctgacag agcactcccc ttcctctgtg ccacggacct ttaagaacgt
     6541 ggtgagggaa ggcatcaaag caggcagggc cgtgtctgtg gagacaggcc tgctgacagg
     6601 catcgaggag atcaagaagt ctcagaacat cttcagcggc accacagcca atcgggagag
     6661 aggctggaag aaggccgagg agaagtacgt gtgccacttt accccctgta aggatgagat
     6721 gggcagggtc ggctgggtgg tgctgactgt cgctcctaaa ctggaaagat ttgaattcgg
     6781 tggtggatca ggtggaggat ctatggtgag caagggcgag gaggataaca tggccatcat
     6841 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     6901 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     6961 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     7021 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     7081 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     7141 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     7201 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     7261 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     7321 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     7381 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     7441 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     7501 gctgtacaag ggctcttccg ggacaggctc ctccggcact agtcgaaggc gccacatcgt
     7561 tcggaagcgc acgctgcgga ggctgctgca ggagagggag cttgtggagc ctcttacacc
     7621 cagtggagaa gctcccaacc aagctctctt gaggatcttg aaggaaactg aattcaaaaa
     7681 gatcaaagtg ctgggctccg gtgcgttcgg cacggtgtat aagggactct ggatcccaga
     7741 aggtgagaaa gttaaaattc ccgtcgctat caaggaatta agagaagcaa catctccgaa
     7801 agccaacaag gaaatcctcg atgaagccta cgtgatggcc agcgtggaca acccccacgt
     7861 gtgccgcctg ctgggcatct gcctcacctc caccgtgcaa ctcatcacgc agctcatgcc
     7921 cttcggctgc ctcctggact atgtccggga acacaaagac aatattggct cccagtacct
     7981 gctcaactgg tgtgtgcaga tcgcaaaggg catgaactac ttggaggacc gtcgcttggt
     8041 gcaccgcgac ctggcagcca ggaacgtact ggtgaaaaca ccgcagcatg tcaagatcac
     8101 agattttggg ctggccaaac tgctgggtgc ggaagagaaa gaataccatg cagaaggagg
     8161 caaagtgcct atcaagtgga tggcattgga atcaatttta cacagaatct atacccacca
     8221 gagtgatgtc tggagctacg gggtgaccgt ttgggagttg atgacctttg gatccaagcc
     8281 atatgacgga atccctgcca gcgagatctc ctccatcctg gagaaaggag aacgcctccc
     8341 tcagccaccc atatgtacca tcgatgtcta catgatcatg gtcaagtgct ggatgataga
     8401 cgcagatagt cgcccaaagt tccgtgagtt gatcatcgaa ttctccaaaa tggcccgaga
     8461 cccccagcgc taccttgtca ttcaggggga tgaaagaatg catttgccaa gtcctacaga
     8521 ctccaacttc taccgtgccc tgatggatga agaagacatg gacgacgtgg tggatgccga
     8581 cgagtacctc atcccacagc agggcttctt cagcagcccc tccacgtcac ggactcccct
     8641 cctgagctct ctgagtgcaa ccagcaacaa ttccaccgtg gcttgcattg atagaaatgg
     8701 gctgcaaagc tgtcccatca aggaagacag cttcttgcag cgatacagct cagaccccac
     8761 aggcgccttg actgaggaca gcatagacga caccttcctc ccagtgcctg aatacataaa
     8821 ccagtccgtt cccaaaaggc ccgctggctc tgtgcagaat cctgtctatc acaatcagcc
     8881 tctgaacccc gcgcccagca gagacccaca ctaccaggac ccccacagca ctgcagtggg
     8941 caaccccgag tatctcaaca ctgtccagcc cacctgtgtc aacagcacat tcgacagccc
     9001 tgcccactgg gcccagaaag gcagccacca aattagcctg gacaaccctg actaccagca
     9061 ggacttcttt cccaaggaag ccaagccaaa tggcatcttt aagggctcca cagctgaaaa
     9121 tgcagaatac ctaagggtcg cgccacaaag cagtgaattt attggagcat aatctagagg
     9181 gcccgtttaa acccggcggc cgcaactccc acctgcaaca tgcgtgactg actgaggccg
     9241 cgactctaga gtcgacctgc aggcatgcaa gcttgatatc aagcttatcg ataatcaacc
     9301 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     9361 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     9421 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     9481 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     9541 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     9601 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     9661 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     9721 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     9781 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     9841 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
     9901 agggaattaa tttgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     9961 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
    10021 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
    10081 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
    10141 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
    10201 agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca
    10261 cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga
    10321 caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct
    10381 ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc
    10441 cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct
    10501 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
    10561 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
    10621 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
    10681 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
    10741 cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct
    10801 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
    10861 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
    10921 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
    10981 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
    11041 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    11101 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
    11161 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    11221 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    11281 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    11341 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    11401 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    11461 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    11521 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    11581 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    11641 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    11701 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    11761 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    11821 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    11881 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    11941 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    12001 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    12061 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    12121 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    12181 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    12241 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    12301 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    12361 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    12421 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    12481 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    12541 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    12601 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    12661 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    12721 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    12781 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    12841 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    12901 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    12961 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
    13021 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    13081 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
    13141 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
    13201 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gc
//
