LOCUS       sequence-415153-       13744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Fus-BcLOV-EGFR for optogenetic activation of EGFR 
            signaling. Includes an mCherry tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13744)
  AUTHORS   Pal AA, Benman W, Mumford TR, Huang Z, Chow BY, Bugaj LJ
  TITLE     Optogenetic clustering and membrane translocation of the BcLOV4 
            photoreceptor.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Aug 8;120(32):e2221615120. doi: 
            10.1073/pnas.2221615120. Epub 2023 Aug 1.
  PUBMED    37527339
REFERENCE   2  (bases 1 to 13744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-08-8"; volume: "120(32):e2221615120. 
            doi"; pages: " 10.1073/pnas.2221615120. Epub 2023 Aug 1"
COMMENT     Sequence Label: Fus-BcLOV-mCh-EGFR
FEATURES             Location/Qualifiers
     source          1..13744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..237
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(307..327)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        312..641
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      492..627
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1775..1840
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1970..1990
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2415..2549
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2452..2471)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2506..2525
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2717..3350
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3397..3522
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4019..4252
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4437..4481
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4766..4883
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4964..5267
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5268..5471
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5422..5442
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      5509..5518
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5895..5906
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             7834..8541
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7979..7997)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8247..8266
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    10324..10912
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10377..10397)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10795..10806)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10915..10931)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10916..10932)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             11022..11255
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(11280..11298)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(11281..11298)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(11588..11607)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(11821..11840)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11940..11962
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12000..12018)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12086..12190
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12191..12259,12260..13051)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      join(13222..13744,1..66)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13711..13730
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
       61 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
      121 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
      181 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
      241 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
      301 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
      361 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
      421 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
      481 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
      541 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
      601 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag
      661 gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa
      721 aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg
      781 caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac
      841 atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca
      901 agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg
      961 taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt
     1021 ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg
     1081 gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc
     1141 gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc
     1201 tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata
     1261 tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc
     1321 cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag
     1381 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat
     1441 cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
     1501 gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
     1561 tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat
     1621 gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg
     1681 atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
     1741 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
     1801 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
     1861 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
     1921 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
     1981 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
     2041 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
     2101 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
     2161 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
     2221 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
     2281 atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg
     2341 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     2401 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     2461 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     2521 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
     2581 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
     2641 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
     2701 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     2761 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     2821 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     2881 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     2941 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     3001 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     3061 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     3121 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     3181 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     3241 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     3301 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     3361 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     3421 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     3481 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     3541 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     3601 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     3661 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     3721 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     3781 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     3841 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
     3901 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     3961 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     4021 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     4081 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     4141 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     4201 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     4261 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     4321 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     4381 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     4441 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     4501 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     4561 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     4621 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     4681 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     4741 gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     4801 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     4861 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga
     4921 tcgataagct tgatatcgaa ttcagcccat atatggagtt ccgcgttaca taacttacgg
     4981 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     5041 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     5101 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     5161 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     5221 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     5281 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     5341 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     5401 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     5461 taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctacgcgtgc caccatggcc
     5521 tcaaacgatt atacccaaca agcaacccaa agctatgggg cctaccccac ccagcccggg
     5581 cagggctatt cccagcagag cagtcagccc tacggacagc agagttacag tggttatagc
     5641 cagtccacgg acacttcagg ctatggccag agcagctatt cttcttatgg ccagagccag
     5701 aacacaggct atggaactca gtcaactccc cagggatatg gctcgactgg cggctatggc
     5761 agtagccaga gctcccaatc gtcttacggg cagcagtcct cctatcctgg ctatggccag
     5821 cagccagctc ccagcagcac ctcgggaagt tacggtagca gttctcagag cagcagctat
     5881 gggcagcccc agagtgggag ctacagccag cagcctagct atggtggaca gcagcaaagc
     5941 tatggacagc agcaaagcta taatccccct cagggctatg gacagcagaa ccagtacaac
     6001 agcggtagcg gcagcggtag catggccaca gacgcaatcg accattacta ccagaacaga
     6061 gacggccccc cttccaggag ctttagcgac gaatacgact catacaataa gacagcccct
     6121 ctgccacctc tgagcgcctg cccagagcgc gcccagaccc ctaacagcaa gagaatcccc
     6181 atcacaccca attccgccaa gagcacctcc gagtctcctc ggagaatcac attctctcgc
     6241 gacgcaggca gctccaccaa gggcagcacc cagacaagcc catccaacca gggctccgtg
     6301 tctagccgga atggctctaa cggaaatggc ctgggcctgt cctctgccgg caacttcgcc
     6361 gatttcttta gctccgaggt gttttatatc gtgctgcaca atcccaccac agcccacaga
     6421 ttcctgaagt tttgccagag cagggcctgt tccgagaacc tggagtttct gcagaagatc
     6481 gacgagtaca ataggctgat cgatgaggcc acacgcctgc tgtctaccat ctacagcaca
     6541 tacgtgagca gcgaggcccc taagcagatc aacatctcta atagccacgc caggcgcctg
     6601 agccacgaca tcaagggagc aacccacaac gtgctgcccg gcctggagga tatcttcaat
     6661 ggctcccagg agcagatcga gaagctgctg gcctctgacc tgtatcctag atttgtgaag
     6721 caccaggtga ccgcatccgc cacaatggcc ctggcaaacg acaaggagag gtacccaggc
     6781 ctgggcgatt gcttctgtct gacagaccca cggagagccg ataatcccat cgtgttcgca
     6841 agcgacggat tcgtgagcgt gaccggctat tcccgctctg atatcatccc aaggaactgc
     6901 cgcttcctgc agggcagctt taccgaccgg caggccacaa agaggctgcg cacctccatc
     6961 gagaatcgcg aggagacagt ggagctgctg ctgaactatc ggaagaatgg cgatcccttt
     7021 tggaacctgc tgtacgtgtc ccctctgctg gacggcaatg gcgatgtgag attctttctg
     7081 ggcggccaga tcaactgttc taccacaatc cacagccgga ccgacgtgct gagaatcctg
     7141 agcctgaacg acgaggatac aggctccttc atcgagggct ctaataagac ccccagcgtg
     7201 cggtccaagg agtctaacaa tgcctccaac tatggcaaga catccttctt taagtctttt
     7261 aagaagtaca aggccaatac cgtggagatc agagacgagg ccggcatgga gggcgagctg
     7321 atcgatagga tcggcaagct gcagttcaag acacaggtgg aggagtttta caccgcctat
     7381 tctaagtacc tggtgctgag ctatcagcca cagtcccaga acctgatcgt gaagtacttc
     7441 agccccggca tcgtggatat gctgaacctg aatatgccta atggcctggt ggccccaatc
     7501 gtggacaagg atatcttcaa ggtgctgaca gagcactccc cttcctctgt gccacggacc
     7561 tttaagaacg tggtgaggga aggcatcaaa gcaggcaggg ccgtgtctgt ggagacaggc
     7621 ctgctgacag gcatcgagga gatcaagaag tctcagaaca tcttcagcgg caccacagcc
     7681 aatcgggaga gaggctggaa gaaggccgag gagaagtacg tgtgccactt taccccctgt
     7741 aaggatgaga tgggcagggt cggctgggtg gtgctgactg tcgctcctaa actggaaaga
     7801 tttgaattcg gtggtggatc aggtggagga tctatggtga gcaagggcga ggaggataac
     7861 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     7921 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     7981 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     8041 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     8101 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     8161 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     8221 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     8281 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     8341 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     8401 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     8461 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     8521 ggcatggacg agctgtacaa gggctcttcc gggacaggct cctccggcac tagtcgaagg
     8581 cgccacatcg ttcggaagcg cacgctgcgg aggctgctgc aggagaggga gcttgtggag
     8641 cctcttacac ccagtggaga agctcccaac caagctctct tgaggatctt gaaggaaact
     8701 gaattcaaaa agatcaaagt gctgggctcc ggtgcgttcg gcacggtgta taagggactc
     8761 tggatcccag aaggtgagaa agttaaaatt cccgtcgcta tcaaggaatt aagagaagca
     8821 acatctccga aagccaacaa ggaaatcctc gatgaagcct acgtgatggc cagcgtggac
     8881 aacccccacg tgtgccgcct gctgggcatc tgcctcacct ccaccgtgca actcatcacg
     8941 cagctcatgc ccttcggctg cctcctggac tatgtccggg aacacaaaga caatattggc
     9001 tcccagtacc tgctcaactg gtgtgtgcag atcgcaaagg gcatgaacta cttggaggac
     9061 cgtcgcttgg tgcaccgcga cctggcagcc aggaacgtac tggtgaaaac accgcagcat
     9121 gtcaagatca cagattttgg gctggccaaa ctgctgggtg cggaagagaa agaataccat
     9181 gcagaaggag gcaaagtgcc tatcaagtgg atggcattgg aatcaatttt acacagaatc
     9241 tatacccacc agagtgatgt ctggagctac ggggtgaccg tttgggagtt gatgaccttt
     9301 ggatccaagc catatgacgg aatccctgcc agcgagatct cctccatcct ggagaaagga
     9361 gaacgcctcc ctcagccacc catatgtacc atcgatgtct acatgatcat ggtcaagtgc
     9421 tggatgatag acgcagatag tcgcccaaag ttccgtgagt tgatcatcga attctccaaa
     9481 atggcccgag acccccagcg ctaccttgtc attcaggggg atgaaagaat gcatttgcca
     9541 agtcctacag actccaactt ctaccgtgcc ctgatggatg aagaagacat ggacgacgtg
     9601 gtggatgccg acgagtacct catcccacag cagggcttct tcagcagccc ctccacgtca
     9661 cggactcccc tcctgagctc tctgagtgca accagcaaca attccaccgt ggcttgcatt
     9721 gatagaaatg ggctgcaaag ctgtcccatc aaggaagaca gcttcttgca gcgatacagc
     9781 tcagacccca caggcgcctt gactgaggac agcatagacg acaccttcct cccagtgcct
     9841 gaatacataa accagtccgt tcccaaaagg cccgctggct ctgtgcagaa tcctgtctat
     9901 cacaatcagc ctctgaaccc cgcgcccagc agagacccac actaccagga cccccacagc
     9961 actgcagtgg gcaaccccga gtatctcaac actgtccagc ccacctgtgt caacagcaca
    10021 ttcgacagcc ctgcccactg ggcccagaaa ggcagccacc aaattagcct ggacaaccct
    10081 gactaccagc aggacttctt tcccaaggaa gccaagccaa atggcatctt taagggctcc
    10141 acagctgaaa atgcagaata cctaagggtc gcgccacaaa gcagtgaatt tattggagca
    10201 taatctagag ggcccgttta aacccggcgg ccgcaactcc cacctgcaac atgcgtgact
    10261 gactgaggcc gcgactctag agtcgacctg caggcatgca agcttgatat caagcttatc
    10321 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
    10381 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
    10441 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
    10501 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
    10561 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
    10621 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
    10681 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
    10741 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
    10801 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
    10861 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
    10921 cgtcgacctc gagggaatta atttgagctc ggtaccttta agaccaatga cttacaaggc
    10981 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
    11041 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
    11101 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    11161 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    11221 cagacccttt tagtcagtgt ggaaaatctc tagcagcatc tagaattaat tccgtgtatt
    11281 ctatagtgtc acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg
    11341 cgttctaacg acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc
    11401 ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt
    11461 tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct
    11521 agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
    11581 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
    11641 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
    11701 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
    11761 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag
    11821 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
    11881 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
    11941 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
    12001 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
    12061 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
    12121 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
    12181 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
    12241 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
    12301 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
    12361 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
    12421 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
    12481 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
    12541 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
    12601 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
    12661 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
    12721 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
    12781 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
    12841 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
    12901 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
    12961 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
    13021 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
    13081 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    13141 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    13201 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    13261 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    13321 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
    13381 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    13441 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    13501 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    13561 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    13621 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    13681 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    13741 cggg
//
