LOCUS       sequence-415154-       13759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses RGG-BcLOV-EGFR for optogenetic activation of EGFR 
            signaling. Includes an mCherry tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13759)
  AUTHORS   Pal AA, Benman W, Mumford TR, Huang Z, Chow BY, Bugaj LJ
  TITLE     Optogenetic clustering and membrane translocation of the BcLOV4 
            photoreceptor.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Aug 8;120(32):e2221615120. doi: 
            10.1073/pnas.2221615120. Epub 2023 Aug 1.
  PUBMED    37527339
REFERENCE   2  (bases 1 to 13759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-08-8"; volume: "120(32):e2221615120. 
            doi"; pages: " 10.1073/pnas.2221615120. Epub 2023 Aug 1"
COMMENT     Sequence Label: RGG-BcLOV-mCh-EGFR
FEATURES             Location/Qualifiers
     source          1..13759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2031..2738
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRNSTGGMDELYK"
     primer_bind     complement(2176..2194)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2444..2463
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4521..5109
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4574..4594)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4992..5003)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5112..5128)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5113..5129)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5219..5452
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5477..5495)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5478..5495)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5785..5804)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6018..6037)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6137..6159
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6197..6215)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6283..6387
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6388..6456,6457..7248)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7419..8007
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7908..7927
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8161..8178
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8248..8268)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8253..8582
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8433..8568
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9716..9781
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9911..9931
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10356..10490
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10393..10412)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10447..10466
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10658..11291
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11338..11463
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11960..12193
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12378..12422
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12707..12824
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        12905..13208
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        13209..13412
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     13363..13383
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      13450..13459
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggggtggtgg gggaggaggc aatcgcggct acaataataa ccgcggtggg ggtggaggcg
       61 gctataatcg gcaggaccgt ggagacggcg gttctagtaa tttctcgcgg ggagggtata
      121 acaacagaga tgagggctca gacaacagag gctcaggcag atcatacaac aacgatcggc
      181 gcgacaatgg tggtgacggg ggtagcggca gcggtagcat ggccacagac gcaatcgacc
      241 attactacca gaacagagac ggcccccctt ccaggagctt tagcgacgaa tacgactcat
      301 acaataagac agcccctctg ccacctctga gcgcctgccc agagcgcgcc cagaccccta
      361 acagcaagag aatccccatc acacccaatt ccgccaagag cacctccgag tctcctcgga
      421 gaatcacatt ctctcgcgac gcaggcagct ccaccaaggg cagcacccag acaagcccat
      481 ccaaccaggg ctccgtgtct agccggaatg gctctaacgg aaatggcctg ggcctgtcct
      541 ctgccggcaa cttcgccgat ttctttagct ccgaggtgtt ttatatcgtg ctgcacaatc
      601 ccaccacagc ccacagattc ctgaagtttt gccagagcag ggcctgttcc gagaacctgg
      661 agtttctgca gaagatcgac gagtacaata ggctgatcga tgaggccaca cgcctgctgt
      721 ctaccatcta cagcacatac gtgagcagcg aggcccctaa gcagatcaac atctctaata
      781 gccacgccag gcgcctgagc cacgacatca agggagcaac ccacaacgtg ctgcccggcc
      841 tggaggatat cttcaatggc tcccaggagc agatcgagaa gctgctggcc tctgacctgt
      901 atcctagatt tgtgaagcac caggtgaccg catccgccac aatggccctg gcaaacgaca
      961 aggagaggta cccaggcctg ggcgattgct tctgtctgac agacccacgg agagccgata
     1021 atcccatcgt gttcgcaagc gacggattcg tgagcgtgac cggctattcc cgctctgata
     1081 tcatcccaag gaactgccgc ttcctgcagg gcagctttac cgaccggcag gccacaaaga
     1141 ggctgcgcac ctccatcgag aatcgcgagg agacagtgga gctgctgctg aactatcgga
     1201 agaatggcga tcccttttgg aacctgctgt acgtgtcccc tctgctggac ggcaatggcg
     1261 atgtgagatt ctttctgggc ggccagatca actgttctac cacaatccac agccggaccg
     1321 acgtgctgag aatcctgagc ctgaacgacg aggatacagg ctccttcatc gagggctcta
     1381 ataagacccc cagcgtgcgg tccaaggagt ctaacaatgc ctccaactat ggcaagacat
     1441 ccttctttaa gtcttttaag aagtacaagg ccaataccgt ggagatcaga gacgaggccg
     1501 gcatggaggg cgagctgatc gataggatcg gcaagctgca gttcaagaca caggtggagg
     1561 agttttacac cgcctattct aagtacctgg tgctgagcta tcagccacag tcccagaacc
     1621 tgatcgtgaa gtacttcagc cccggcatcg tggatatgct gaacctgaat atgcctaatg
     1681 gcctggtggc cccaatcgtg gacaaggata tcttcaaggt gctgacagag cactcccctt
     1741 cctctgtgcc acggaccttt aagaacgtgg tgagggaagg catcaaagca ggcagggccg
     1801 tgtctgtgga gacaggcctg ctgacaggca tcgaggagat caagaagtct cagaacatct
     1861 tcagcggcac cacagccaat cgggagagag gctggaagaa ggccgaggag aagtacgtgt
     1921 gccactttac cccctgtaag gatgagatgg gcagggtcgg ctgggtggtg ctgactgtcg
     1981 ctcctaaact ggaaagattt gaattcggtg gtggatcagg tggaggatct atggtgagca
     2041 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     2101 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     2161 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     2221 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     2281 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     2341 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     2401 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     2461 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     2521 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     2581 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     2641 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     2701 gccgcaactc caccggcggc atggacgagc tgtacaaggg ctcttccggg acaggctcct
     2761 ccggcactag tcgaaggcgc cacatcgttc ggaagcgcac gctgcggagg ctgctgcagg
     2821 agagggagct tgtggagcct cttacaccca gtggagaagc tcccaaccaa gctctcttga
     2881 ggatcttgaa ggaaactgaa ttcaaaaaga tcaaagtgct gggctccggt gcgttcggca
     2941 cggtgtataa gggactctgg atcccagaag gtgagaaagt taaaattccc gtcgctatca
     3001 aggaattaag agaagcaaca tctccgaaag ccaacaagga aatcctcgat gaagcctacg
     3061 tgatggccag cgtggacaac ccccacgtgt gccgcctgct gggcatctgc ctcacctcca
     3121 ccgtgcaact catcacgcag ctcatgccct tcggctgcct cctggactat gtccgggaac
     3181 acaaagacaa tattggctcc cagtacctgc tcaactggtg tgtgcagatc gcaaagggca
     3241 tgaactactt ggaggaccgt cgcttggtgc accgcgacct ggcagccagg aacgtactgg
     3301 tgaaaacacc gcagcatgtc aagatcacag attttgggct ggccaaactg ctgggtgcgg
     3361 aagagaaaga ataccatgca gaaggaggca aagtgcctat caagtggatg gcattggaat
     3421 caattttaca cagaatctat acccaccaga gtgatgtctg gagctacggg gtgaccgttt
     3481 gggagttgat gacctttgga tccaagccat atgacggaat ccctgccagc gagatctcct
     3541 ccatcctgga gaaaggagaa cgcctccctc agccacccat atgtaccatc gatgtctaca
     3601 tgatcatggt caagtgctgg atgatagacg cagatagtcg cccaaagttc cgtgagttga
     3661 tcatcgaatt ctccaaaatg gcccgagacc cccagcgcta ccttgtcatt cagggggatg
     3721 aaagaatgca tttgccaagt cctacagact ccaacttcta ccgtgccctg atggatgaag
     3781 aagacatgga cgacgtggtg gatgccgacg agtacctcat cccacagcag ggcttcttca
     3841 gcagcccctc cacgtcacgg actcccctcc tgagctctct gagtgcaacc agcaacaatt
     3901 ccaccgtggc ttgcattgat agaaatgggc tgcaaagctg tcccatcaag gaagacagct
     3961 tcttgcagcg atacagctca gaccccacag gcgccttgac tgaggacagc atagacgaca
     4021 ccttcctccc agtgcctgaa tacataaacc agtccgttcc caaaaggccc gctggctctg
     4081 tgcagaatcc tgtctatcac aatcagcctc tgaaccccgc gcccagcaga gacccacact
     4141 accaggaccc ccacagcact gcagtgggca accccgagta tctcaacact gtccagccca
     4201 cctgtgtcaa cagcacattc gacagccctg cccactgggc ccagaaaggc agccaccaaa
     4261 ttagcctgga caaccctgac taccagcagg acttctttcc caaggaagcc aagccaaatg
     4321 gcatctttaa gggctccaca gctgaaaatg cagaatacct aagggtcgcg ccacaaagca
     4381 gtgaatttat tggagcataa tctagagggc ccgtttaaac ccggcggccg caactcccac
     4441 ctgcaacatg cgtgactgac tgaggccgcg actctagagt cgacctgcag gcatgcaagc
     4501 ttgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     4561 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4621 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4681 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4741 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4801 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4861 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     4921 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     4981 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5041 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5101 cctccccgca tcgataccgt cgacctcgag ggaattaatt tgagctcggt acctttaaga
     5161 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     5221 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     5281 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     5341 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     5401 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag
     5461 aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta
     5521 tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg
     5581 gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg
     5641 gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa
     5701 tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg
     5761 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     5821 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     5881 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
     5941 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
     6001 gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     6061 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     6121 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     6181 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     6241 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6301 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6361 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6421 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6481 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6541 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6601 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     6661 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6721 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6781 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6841 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6901 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6961 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     7021 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7081 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7141 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7201 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7261 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7321 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7381 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7441 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7501 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7561 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7621 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7681 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7741 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7801 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7861 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7921 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7981 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     8041 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     8101 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     8161 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     8221 cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     8281 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     8341 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     8401 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     8461 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     8521 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     8581 aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg
     8641 tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg
     8701 ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat
     8761 aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag
     8821 atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt
     8881 gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc
     8941 atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc
     9001 tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg
     9061 gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg
     9121 caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct
     9181 ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat
     9241 cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag
     9301 tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg
     9361 ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc
     9421 gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg
     9481 attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag
     9541 gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt
     9601 ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta
     9661 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa
     9721 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta
     9781 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa
     9841 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca
     9901 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt
     9961 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta
    10021 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac
    10081 ctttataagt aggcataaca gttataatca taacatactg ttttttctta ctccacacag
    10141 gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat
    10201 ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca
    10261 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
    10321 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
    10381 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    10441 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
    10501 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
    10561 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    10621 attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa
    10681 agaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
    10741 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
    10801 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
    10861 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
    10921 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
    10981 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
    11041 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
    11101 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
    11161 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
    11221 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
    11281 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
    11341 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
    11401 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
    11461 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
    11521 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
    11581 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
    11641 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
    11701 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
    11761 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca
    11821 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
    11881 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
    11941 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
    12001 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
    12061 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
    12121 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
    12181 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
    12241 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
    12301 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
    12361 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
    12421 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
    12481 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
    12541 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
    12601 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
    12661 tgaacggatc tcgacggtat cgccaaatgg cagtattcat ccacaatttt aaaagaaaag
    12721 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
    12781 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
    12841 acagcagaga tccagtttgg atcgataagc ttgatatcga attcagccca tatatggagt
    12901 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
    12961 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
    13021 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
    13081 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
    13141 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
    13201 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
    13261 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
    13321 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
    13381 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc
    13441 gctacgcgtg ccaccatgga atcgaatcaa tctaacaacg gtggatcggg aaacgcagca
    13501 ttgaatcgtg ggggccgtta cgtaccacca cacttgagag gcggagatgg tggtgccgcc
    13561 gctgctgcct cagccggagg cgacgacaga cgcggtgggg ccgggggtgg tggctatcgt
    13621 agaggtggtg gaaattcagg gggcggcggc ggaggaggct acgatcgtgg atacaacgac
    13681 aaccgggatg accgtgacaa tcggggcggc tccggcggtt atggccggga cagaaattac
    13741 gaggatcgcg gttataacg
//
