LOCUS       sequence-415179-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of N-terminally tagged GABARAP with mTagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Uffing A, Gold L, Gensch T, Weiergraber OH, Hoffmann S, Willbold D
  TITLE     Highlighting the hidden: monitoring the avidity-driven association 
            of a fluorescent GABARAP tandem with microtubules in living cells.
  JOURNAL   Autophagy Rep. 2024 May 16;3(1):2348899. doi: 
            10.1080/27694127.2024.2348899. eCollection 2024.
  PUBMED    40395516
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Autophagy 
            Rep."; date: "2024-05-16"; volume: "3(1):2348899. doi"; pages: " 
            10.1080/27694127.2024.2348899. eCollection 2024"
COMMENT     Sequence Label: pcDNA5/FRT/TO mTagBFP2-GABARAP
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3..21
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    24..42
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     52..76
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             159..869
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 tctccctatc agtgatagag atctccctat cagtgataga gatcgtcgac gagctcgttt
       61 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
      121 ccgggaccga tccagcctcc ggactctagc gtttaaacat ggtgtctaag ggcgaagagc
      181 tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac aaccatcact
      241 tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca
      301 aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact agcttcctct
      361 acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc aagcagtcct
      421 tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg
      481 ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg
      541 tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccttca
      601 ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg gccctgaagc
      661 tcgtgggcgg gagccatctg atcgcaaacg ccaagaccac atatagatcc aagaaacccg
      721 ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa agaatcaagg
      781 aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga tactgcgacc
      841 tccctagcaa actggggcac aagcttaatt ccggactcag atctcgagct atgaagttcg
      901 tgtacaaaga agagcatccg ttcgagaagc gccgctctga gggcgagaag atccgaaaga
      961 aatacccgga ccgggtgccg gtgatagtag aaa
//
