LOCUS       sequence-41524-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Rishi V, Gal J, Krylov D, Fridriksson J, Boysen MS, Mandrup S, 
            Vinson C
  TITLE     SREBP-1 dimerization specificity maps to both the helix-loop-helix 
            and leucine zipper domains: use of a dominant negative.
  JOURNAL   J Biol Chem. 2004 Mar 19;279(12):11863-74. Epub 2003 Dec 31.
  PUBMED    14702347
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-03-19"; volume: "279(12)"; pages: "11863-74. 
            Epub 2003 Dec 31"
COMMENT     Sequence Label: CMV500 A-SREBP1
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             89..112
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             116..148
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(385..403)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(386..403)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(423..440)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    429..654
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(787..806)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ccaccatgga ctacaaggac gacgatgaca agcatatggc
      121 tagcatgact ggtggacagc aaatgggtcg ggatcccgac gaagaggaag atgacgaaga
      181 agaactcgag gaactggaag acagcattgt ggagctcaaa gacctggtgg tgggcactga
      241 ggcaaagctg aataaatctg ccgtcttgcg caaggccatc gactatatcc gcttcttaca
      301 gcacagcaac cagaagctca agcaggagaa cctggccctg cgaaatgccg ctcacaaaag
      361 ctaaaagctt ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc
      421 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
      481 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
      541 gcattgtctg agtaggtgtc attctattct gggggggtgg ggtggggcag gacagcaagg
      601 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
      661 aggcggaaag aaccagctgg ggctcgaggg gggatcccca cgcgccctgt agcggcgcat
      721 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      781 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      841 aagctctaa
//
