LOCUS       sequence-41525-e         585 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Koo BK, Stange DE, Sato T, Karthaus W, Farin HF, Huch M, van Es JH, 
            Clevers H
  TITLE     Controlled gene expression in primary Lgr5 organoid cultures.
  JOURNAL   Nat Methods. 2011 Dec 4. doi: 10.1038/nmeth.1802.
  PUBMED    22138822
REFERENCE   2  (bases 1 to 585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2011 Dec 4. doi: 10.1038/nmeth.1802."
COMMENT     Sequence Label: pMSCV-FLIP-puro-dsRed-GFP-miRNA
FEATURES             Location/Qualifiers
     source          1..585
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           40..66
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           130..171
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     protein_bind    236..269
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(342..365)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(540..559)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgatcgttta aagggaggta gtgagtcgac cagtggatcc tggaggcttg ctgaaggctg
       61 tatgctgtcg ttcatgcact cgctgaaggt tttggccact gactgacctt cagcgtgcat
      121 gaacgacagg acacaaggcc tgttactagc actcacatgg aacaaatggc ccagatctgg
      181 ccgcactcga gtttaaaccc ctgactctcc cctttttttt ttcctccagg tatgcataac
      241 ttcgtataat gtgtactata cgaagttatg gcttctagac tactggaaca ggtggtggcg
      301 ggcctcggcg cgctcgtact gctccaccac ggtgtagtcc tcgttgtggg aggtgatgtc
      361 cagcttggag tccacgtagt agtagccggg cagcttcacg ggcttcttgg ccatgtanat
      421 tgacttgaac tccaccaggt agtggccgcc gcccttcagc ttcagcgcct tgtggatctc
      481 gcccttcagc acgccgtcgc gggggtacag gcgctcggtg gagggctccc agcccagagt
      541 cttcttctgc attacggggc cgtcggaggg gaagttcacg cccaa
//
