LOCUS       sequence-415313-       13521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses RGG-BcLOV-SOS for optogenetic activation of Ras/Erk 
            signaling. Includes an mCherry tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13521)
  AUTHORS   Pal AA, Benman W, Mumford TR, Huang Z, Chow BY, Bugaj LJ
  TITLE     Optogenetic clustering and membrane translocation of the BcLOV4 
            photoreceptor.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Aug 8;120(32):e2221615120. doi: 
            10.1073/pnas.2221615120. Epub 2023 Aug 1.
  PUBMED    37527339
REFERENCE   2  (bases 1 to 13521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-08-8"; volume: "120(32):e2221615120. 
            doi"; pages: " 10.1073/pnas.2221615120. Epub 2023 Aug 1"
COMMENT     Sequence Label: RGG-BcLOV-mCh-SOS
FEATURES             Location/Qualifiers
     source          1..13521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2023..2730
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2168..2186)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2436..2455
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4313..4901
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4366..4386)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4784..4795)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4904..4920)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4905..4921)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5011..5244
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5269..5287)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5270..5287)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5577..5596)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5810..5829)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5929..5951
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5989..6007)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6075..6179
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6180..6248,6249..7040)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6398..6417)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7211..7799
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7700..7719
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7953..7970
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8040..8060)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8045..8374
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8225..8360
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9508..9573
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9703..9723
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10148..10282
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10185..10204)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10239..10258
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10450..11083
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11130..11255
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11752..11985
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12170..12214
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12499..12616
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        12769..13176
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
ORIGIN
        1 gggggaggag gcaatcgcgg ctacaataat aaccgcggtg ggggtggagg cggctataat
       61 cggcaggacc gtggagacgg cggttctagt aatttctcgc ggggagggta taacaacaga
      121 gatgagggct cagacaacag aggctcaggc agatcataca acaacgatcg gcgcgacaat
      181 ggtggtgacg ggggtagcgg cagcggtagc atggccacag acgcaatcga ccattactac
      241 cagaacagag acggcccccc ttccaggagc tttagcgacg aatacgactc atacaataag
      301 acagcccctc tgccacctct gagcgcctgc ccagagcgcg cccagacccc taacagcaag
      361 agaatcccca tcacacccaa ttccgccaag agcacctccg agtctcctcg gagaatcaca
      421 ttctctcgcg acgcaggcag ctccaccaag ggcagcaccc agacaagccc atccaaccag
      481 ggctccgtgt ctagccggaa tggctctaac ggaaatggcc tgggcctgtc ctctgccggc
      541 aacttcgccg atttctttag ctccgaggtg ttttatatcg tgctgcacaa tcccaccaca
      601 gcccacagat tcctgaagtt ttgccagagc agggcctgtt ccgagaacct ggagtttctg
      661 cagaagatcg acgagtacaa taggctgatc gatgaggcca cacgcctgct gtctaccatc
      721 tacagcacat acgtgagcag cgaggcccct aagcagatca acatctctaa tagccacgcc
      781 aggcgcctga gccacgacat caagggagca acccacaacg tgctgcccgg cctggaggat
      841 atcttcaatg gctcccagga gcagatcgag aagctgctgg cctctgacct gtatcctaga
      901 tttgtgaagc accaggtgac cgcatccgcc acaatggccc tggcaaacga caaggagagg
      961 tacccaggcc tgggcgattg cttctgtctg acagacccac ggagagccga taatcccatc
     1021 gtgttcgcaa gcgacggatt cgtgagcgtg accggctatt cccgctctga tatcatccca
     1081 aggaactgcc gcttcctgca gggcagcttt accgaccggc aggccacaaa gaggctgcgc
     1141 acctccatcg agaatcgcga ggagacagtg gagctgctgc tgaactatcg gaagaatggc
     1201 gatccctttt ggaacctgct gtacgtgtcc cctctgctgg acggcaatgg cgatgtgaga
     1261 ttctttctgg gcggccagat caactgttct accacaatcc acagccggac cgacgtgctg
     1321 agaatcctga gcctgaacga cgaggataca ggctccttca tcgagggctc taataagacc
     1381 cccagcgtgc ggtccaagga gtctaacaat gcctccaact atggcaagac atccttcttt
     1441 aagtctttta agaagtacaa ggccaatacc gtggagatca gagacgaggc cggcatggag
     1501 ggcgagctga tcgataggat cggcaagctg cagttcaaga cacaggtgga ggagttttac
     1561 accgcctatt ctaagtacct ggtgctgagc tatcagccac agtcccagaa cctgatcgtg
     1621 aagtacttca gccccggcat cgtggatatg ctgaacctga atatgcctaa tggcctggtg
     1681 gccccaatcg tggacaagga tatcttcaag gtgctgacag agcactcccc ttcctctgtg
     1741 ccacggacct ttaagaacgt ggtgagggaa ggcatcaaag caggcagggc cgtgtctgtg
     1801 gagacaggcc tgctgacagg catcgaggag atcaagaagt ctcagaacat cttcagcggc
     1861 accacagcca atcgggagag aggctggaag aaggccgagg agaagtacgt gtgccacttt
     1921 accccctgta aggatgagat gggcagggtc ggctgggtgg tgctgactgt cgctcctaaa
     1981 ctggaaagat ttgaattcgg tggtggatca ggtggaggat ctatggtgag caagggcgag
     2041 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     2101 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     2161 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     2221 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     2281 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     2341 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     2401 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     2461 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     2521 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     2581 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     2641 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     2701 tccaccggcg gcatggacga gctgtacaag ggtggtggat caggtggagg atctcaacca
     2761 ctgagattac caagtcctga agtatatcgt tttgtagtaa aagactctga ggaaaacatt
     2821 gtttttgaag acaacttgca aagtagaagt ggcatcccca ttattaaagg aggaactgta
     2881 gtgaaattaa ttgaaaggtt aacatatcat atgtatgcag atcccaattt tgttcgtact
     2941 tttcttacca catatcgttc attttgtaaa ccacaggaat tgctgagctt actgattgaa
     3001 cggtttgaaa ttccagagcc agaacctact gacgcagaca aattggcaat agagaaaggc
     3061 gagcagccaa tcagtgcaga ccttaaaaga tttcgcaagg aatatgtcca accagtacaa
     3121 cttaggatct taaatgtatt tcggcattgg gttgaacatc atttttatga ctttgaaaga
     3181 gacttggaat tgcttgaaag actagaatcc ttcatttcaa gtgtaagagg gaaagctatg
     3241 aaaaaatggg tagagtcaat tgctaagatc atcaggagga agaagcaagc tcaggcaaac
     3301 ggagtaagcc ataatattac ctttgaaagt ccacctccac caattgaatg gcatatcagc
     3361 aaaccaggac agtttgaaac atttgatctc atgacacttc atccaataga aattgcacgt
     3421 cagctgacac ttttggagtc tgatctttac aggaaagttc aaccgtctga acttgtaggg
     3481 agtgtgtgga ccaaagaaga taaagaaata aattctccaa atttattaaa aatgattcgc
     3541 cataccacaa atctcaccct ctggtttgaa aaatgcattg tggaagcaga aaattttgaa
     3601 gaacgggtgg cagtactaag tagaattata gaaattctgc aagtttttca agatttgaat
     3661 aatttcaatg gcgtattgga gatagtcagt gcagtaaatt cagtgtcagt atacagacta
     3721 gaccatacct ttgaggcact gcaggaaagg aaaaggaaaa ttttggacga agctgtggaa
     3781 ttaagtcaag atcactttaa aaaataccta gtaaaactta agtcaatcaa tccaccttgt
     3841 gtgccttttt ttggaatata tttaacaaat attctgaaga ccgaagaagg gaataatgat
     3901 tttttaaaaa agaaagggaa agatttaatc aatttcagta agaggaggaa agtagctgaa
     3961 attactggag aaattcagca gtatcagaat cagccttact gtttacggat agaaccagat
     4021 atgaggagat tctttgaaaa ccttaacccc atgggaagtg catctgaaaa agagtttaca
     4081 gattatttgt tcaacaagtc actagaaatt gaacctcgaa actgcaaaca gccacctcga
     4141 tttcctagga aatcaacttt ttccttaaaa tctcctggaa taaggcctaa cacaggccga
     4201 catggctcta cctaagcggc cgcaactccc acctgcaaca tgcgtgactg actgaggccg
     4261 cgactctaga gtcgacctgc aggcatgcaa gcttgatatc aagcttatcg ataatcaacc
     4321 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     4381 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     4441 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4501 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4561 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     4621 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4681 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     4741 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     4801 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     4861 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
     4921 agggaattaa tttgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     4981 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     5041 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     5101 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     5161 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     5221 agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca
     5281 cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga
     5341 caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct
     5401 ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc
     5461 cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct
     5521 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
     5581 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
     5641 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
     5701 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
     5761 cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct
     5821 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     5881 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     5941 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     6001 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     6061 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6121 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6181 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6241 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     6301 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     6361 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6421 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6481 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6541 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6601 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6661 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6721 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6781 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     6841 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     6901 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     6961 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     7021 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7081 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7141 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7201 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7261 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7321 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     7381 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7441 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7501 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7561 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7621 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7681 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7741 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7801 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7861 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     7921 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     7981 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
     8041 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     8101 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     8161 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     8221 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     8281 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     8341 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
     8401 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
     8461 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
     8521 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
     8581 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
     8641 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
     8701 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
     8761 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
     8821 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
     8881 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
     8941 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
     9001 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
     9061 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
     9121 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
     9181 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
     9241 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
     9301 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
     9361 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
     9421 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
     9481 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
     9541 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
     9601 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
     9661 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
     9721 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
     9781 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
     9841 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
     9901 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat
     9961 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg
    10021 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
    10081 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    10141 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    10201 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    10261 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
    10321 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
    10381 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
    10441 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
    10501 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
    10561 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
    10621 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
    10681 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
    10741 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
    10801 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
    10861 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
    10921 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
    10981 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
    11041 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
    11101 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
    11161 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
    11221 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
    11281 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
    11341 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
    11401 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
    11461 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
    11521 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
    11581 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
    11641 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
    11701 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
    11761 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
    11821 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
    11881 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
    11941 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
    12001 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
    12061 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
    12121 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
    12181 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
    12241 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
    12301 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
    12361 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
    12421 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat
    12481 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
    12541 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
    12601 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa
    12661 gcttgatatc gaattcctgc agccccgata aaataaaaga ttttatttag tctccagaaa
    12721 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagctgcagt aacgccattt
    12781 tgcaaggcat ggaaaaatac caaaccaaga atagagaagt tcagatcaag ggcgggtaca
    12841 tgaaaatagc taacgttggg ccaaacagga tatctgcggt gagcagtttc ggccccggcc
    12901 cggggccaag aacagatggt caccgcagtt tcggccccgg cccgaggcca agaacagatg
    12961 gtccccagat atggcccaac cctcagcagt ttcttaagac ccatcagatg tttccaggct
    13021 cccccaagga cctgaaatga ccctgcgcct tatttgaatt aaccaatcag cctgcttctc
    13081 gcttctgttc gcgcgcttct gcttcccgag ctctataaaa gagctcacaa cccctcactc
    13141 ggcgcgccag tcctccgaca gactgagtcg cccggggggg atctggagct ctcgagaatt
    13201 ctcacgcgta tggaatcgaa tcaatctaac aacggtggat cgggaaacgc agcattgaat
    13261 cgtgggggcc gttacgtacc accacacttg agaggcggag atggtggtgc cgccgctgct
    13321 gcctcagccg gaggcgacga cagacgcggt ggggccgggg gtggtggcta tcgtagaggt
    13381 ggtggaaatt cagggggcgg cggcggagga ggctacgatc gtggatacaa cgacaaccgg
    13441 gatgaccgtg acaatcgggg cggctccggc ggttatggcc gggacagaaa ttacgaggat
    13501 cgcggttata acgggggtgg t
//
