LOCUS       sequence-415314-       13506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Fus-BcLOV-SOS for optogenetic activation of Ras/Erk 
            signaling. Includes an mCherry tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13506)
  AUTHORS   Pal AA, Benman W, Mumford TR, Huang Z, Chow BY, Bugaj LJ
  TITLE     Optogenetic clustering and membrane translocation of the BcLOV4 
            photoreceptor.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Aug 8;120(32):e2221615120. doi: 
            10.1073/pnas.2221615120. Epub 2023 Aug 1.
  PUBMED    37527339
REFERENCE   2  (bases 1 to 13506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-08-8"; volume: "120(32):e2221615120. 
            doi"; pages: " 10.1073/pnas.2221615120. Epub 2023 Aug 1"
COMMENT     Sequence Label: Fus-BcLOV-mCh-SOS
FEATURES             Location/Qualifiers
     source          1..13506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1236..1301
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1431..1451
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1876..2010
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1913..1932)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1967..1986
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2178..2811
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2858..2983
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3480..3713
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3898..3942
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4227..4344
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4497..4904
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             5318..5329
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             7257..7964
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7402..7420)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7670..7689
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    9547..10135
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9600..9620)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10018..10029)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10138..10154)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10139..10155)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10245..10478
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(10503..10521)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10504..10521)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10811..10830)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(11044..11063)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11163..11185
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11223..11241)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11309..11413
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11414..11482,11483..12274)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11632..11651)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12445..13033
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12934..12953
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13187..13204
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(13274..13294)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(13279..13506,1..102)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(13459..13506,1..88)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
       61 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg acacaagaca
      121 ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag gcagtgcgta
      181 aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc
      241 gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca
      301 catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc
      361 aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct
      421 gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt
      481 tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat
      541 ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc ggttgcgatt
      601 cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg
      661 ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca gccgtgggat
      721 atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcactg
      781 ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa gtattctgga
      841 ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca
      901 tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc
      961 ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat
     1021 ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg tgatgagcga
     1081 tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg gcggcaactg
     1141 gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt gtgacataat
     1201 tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat
     1261 aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg
     1321 atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa
     1381 tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga
     1441 agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg
     1501 ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg
     1561 cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca
     1621 gttataatca taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta
     1681 ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg
     1741 aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag
     1801 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     1861 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     1921 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     1981 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
     2041 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
     2101 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
     2161 actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga
     2221 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
     2281 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
     2341 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
     2401 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
     2461 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
     2521 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
     2581 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
     2641 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     2701 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     2761 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     2821 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     2881 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     2941 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     3001 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     3061 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     3121 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     3181 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     3241 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     3301 taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg
     3361 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     3421 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     3481 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     3541 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     3601 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     3661 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     3721 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     3781 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     3841 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     3901 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     3961 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     4021 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     4081 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     4141 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     4201 cgccaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     4261 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     4321 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     4381 atcgataagc ttgatatcga attcctgcag ccccgataaa ataaaagatt ttatttagtc
     4441 tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta gctgcagtaa
     4501 cgccattttg caaggcatgg aaaaatacca aaccaagaat agagaagttc agatcaaggg
     4561 cgggtacatg aaaatagcta acgttgggcc aaacaggata tctgcggtga gcagtttcgg
     4621 ccccggcccg gggccaagaa cagatggtca ccgcagtttc ggccccggcc cgaggccaag
     4681 aacagatggt ccccagatat ggcccaaccc tcagcagttt cttaagaccc atcagatgtt
     4741 tccaggctcc cccaaggacc tgaaatgacc ctgcgcctta tttgaattaa ccaatcagcc
     4801 tgcttctcgc ttctgttcgc gcgcttctgc ttcccgagct ctataaaaga gctcacaacc
     4861 cctcactcgg cgcgccagtc ctccgacaga ctgagtcgcc cgggggggat ctggagctct
     4921 cgagaattct cacgcgtatg gcctcaaacg attataccca acaagcaacc caaagctatg
     4981 gggcctaccc cacccagccc gggcagggct attcccagca gagcagtcag ccctacggac
     5041 agcagagtta cagtggttat agccagtcca cggacacttc aggctatggc cagagcagct
     5101 attcttctta tggccagagc cagaacacag gctatggaac tcagtcaact ccccagggat
     5161 atggctcgac tggcggctat ggcagtagcc agagctccca atcgtcttac gggcagcagt
     5221 cctcctatcc tggctatggc cagcagccag ctcccagcag cacctcggga agttacggta
     5281 gcagttctca gagcagcagc tatgggcagc cccagagtgg gagctacagc cagcagccta
     5341 gctatggtgg acagcagcaa agctatggac agcagcaaag ctataatccc cctcagggct
     5401 atggacagca gaaccagtac aacagcggta gcggcagcgg tagcatggcc acagacgcaa
     5461 tcgaccatta ctaccagaac agagacggcc ccccttccag gagctttagc gacgaatacg
     5521 actcatacaa taagacagcc cctctgccac ctctgagcgc ctgcccagag cgcgcccaga
     5581 cccctaacag caagagaatc cccatcacac ccaattccgc caagagcacc tccgagtctc
     5641 ctcggagaat cacattctct cgcgacgcag gcagctccac caagggcagc acccagacaa
     5701 gcccatccaa ccagggctcc gtgtctagcc ggaatggctc taacggaaat ggcctgggcc
     5761 tgtcctctgc cggcaacttc gccgatttct ttagctccga ggtgttttat atcgtgctgc
     5821 acaatcccac cacagcccac agattcctga agttttgcca gagcagggcc tgttccgaga
     5881 acctggagtt tctgcagaag atcgacgagt acaataggct gatcgatgag gccacacgcc
     5941 tgctgtctac catctacagc acatacgtga gcagcgaggc ccctaagcag atcaacatct
     6001 ctaatagcca cgccaggcgc ctgagccacg acatcaaggg agcaacccac aacgtgctgc
     6061 ccggcctgga ggatatcttc aatggctccc aggagcagat cgagaagctg ctggcctctg
     6121 acctgtatcc tagatttgtg aagcaccagg tgaccgcatc cgccacaatg gccctggcaa
     6181 acgacaagga gaggtaccca ggcctgggcg attgcttctg tctgacagac ccacggagag
     6241 ccgataatcc catcgtgttc gcaagcgacg gattcgtgag cgtgaccggc tattcccgct
     6301 ctgatatcat cccaaggaac tgccgcttcc tgcagggcag ctttaccgac cggcaggcca
     6361 caaagaggct gcgcacctcc atcgagaatc gcgaggagac agtggagctg ctgctgaact
     6421 atcggaagaa tggcgatccc ttttggaacc tgctgtacgt gtcccctctg ctggacggca
     6481 atggcgatgt gagattcttt ctgggcggcc agatcaactg ttctaccaca atccacagcc
     6541 ggaccgacgt gctgagaatc ctgagcctga acgacgagga tacaggctcc ttcatcgagg
     6601 gctctaataa gacccccagc gtgcggtcca aggagtctaa caatgcctcc aactatggca
     6661 agacatcctt ctttaagtct tttaagaagt acaaggccaa taccgtggag atcagagacg
     6721 aggccggcat ggagggcgag ctgatcgata ggatcggcaa gctgcagttc aagacacagg
     6781 tggaggagtt ttacaccgcc tattctaagt acctggtgct gagctatcag ccacagtccc
     6841 agaacctgat cgtgaagtac ttcagccccg gcatcgtgga tatgctgaac ctgaatatgc
     6901 ctaatggcct ggtggcccca atcgtggaca aggatatctt caaggtgctg acagagcact
     6961 ccccttcctc tgtgccacgg acctttaaga acgtggtgag ggaaggcatc aaagcaggca
     7021 gggccgtgtc tgtggagaca ggcctgctga caggcatcga ggagatcaag aagtctcaga
     7081 acatcttcag cggcaccaca gccaatcggg agagaggctg gaagaaggcc gaggagaagt
     7141 acgtgtgcca ctttaccccc tgtaaggatg agatgggcag ggtcggctgg gtggtgctga
     7201 ctgtcgctcc taaactggaa agatttgaat tcggtggtgg atcaggtgga ggatctatgg
     7261 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     7321 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     7381 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
     7441 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
     7501 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     7561 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     7621 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     7681 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     7741 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     7801 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     7861 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     7921 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagggtggt ggatcaggtg
     7981 gaggatctca accactgaga ttaccaagtc ctgaagtata tcgttttgta gtaaaagact
     8041 ctgaggaaaa cattgttttt gaagacaact tgcaaagtag aagtggcatc cccattatta
     8101 aaggaggaac tgtagtgaaa ttaattgaaa ggttaacata tcatatgtat gcagatccca
     8161 attttgttcg tacttttctt accacatatc gttcattttg taaaccacag gaattgctga
     8221 gcttactgat tgaacggttt gaaattccag agccagaacc tactgacgca gacaaattgg
     8281 caatagagaa aggcgagcag ccaatcagtg cagaccttaa aagatttcgc aaggaatatg
     8341 tccaaccagt acaacttagg atcttaaatg tatttcggca ttgggttgaa catcattttt
     8401 atgactttga aagagacttg gaattgcttg aaagactaga atccttcatt tcaagtgtaa
     8461 gagggaaagc tatgaaaaaa tgggtagagt caattgctaa gatcatcagg aggaagaagc
     8521 aagctcaggc aaacggagta agccataata ttacctttga aagtccacct ccaccaattg
     8581 aatggcatat cagcaaacca ggacagtttg aaacatttga tctcatgaca cttcatccaa
     8641 tagaaattgc acgtcagctg acacttttgg agtctgatct ttacaggaaa gttcaaccgt
     8701 ctgaacttgt agggagtgtg tggaccaaag aagataaaga aataaattct ccaaatttat
     8761 taaaaatgat tcgccatacc acaaatctca ccctctggtt tgaaaaatgc attgtggaag
     8821 cagaaaattt tgaagaacgg gtggcagtac taagtagaat tatagaaatt ctgcaagttt
     8881 ttcaagattt gaataatttc aatggcgtat tggagatagt cagtgcagta aattcagtgt
     8941 cagtatacag actagaccat acctttgagg cactgcagga aaggaaaagg aaaattttgg
     9001 acgaagctgt ggaattaagt caagatcact ttaaaaaata cctagtaaaa cttaagtcaa
     9061 tcaatccacc ttgtgtgcct ttttttggaa tatatttaac aaatattctg aagaccgaag
     9121 aagggaataa tgatttttta aaaaagaaag ggaaagattt aatcaatttc agtaagagga
     9181 ggaaagtagc tgaaattact ggagaaattc agcagtatca gaatcagcct tactgtttac
     9241 ggatagaacc agatatgagg agattctttg aaaaccttaa ccccatggga agtgcatctg
     9301 aaaaagagtt tacagattat ttgttcaaca agtcactaga aattgaacct cgaaactgca
     9361 aacagccacc tcgatttcct aggaaatcaa ctttttcctt aaaatctcct ggaataaggc
     9421 ctaacacagg ccgacatggc tctacctaag cggccgcaac tcccacctgc aacatgcgtg
     9481 actgactgag gccgcgactc tagagtcgac ctgcaggcat gcaagcttga tatcaagctt
     9541 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     9601 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     9661 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     9721 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     9781 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     9841 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     9901 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     9961 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
    10021 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
    10081 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
    10141 taccgtcgac ctcgagggaa ttaatttgag ctcggtacct ttaagaccaa tgacttacaa
    10201 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
    10261 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
    10321 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
    10381 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
    10441 cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt
    10501 attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag
    10561 ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag
    10621 accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga
    10681 gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc
    10741 gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
    10801 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
    10861 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
    10921 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
    10981 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct
    11041 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
    11101 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
    11161 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
    11221 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
    11281 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
    11341 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
    11401 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
    11461 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
    11521 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
    11581 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
    11641 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
    11701 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
    11761 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
    11821 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
    11881 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
    11941 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
    12001 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
    12061 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
    12121 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
    12181 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
    12241 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
    12301 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    12361 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    12421 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    12481 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    12541 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
    12601 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    12661 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    12721 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    12781 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    12841 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    12901 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    12961 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    13021 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    13081 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    13141 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
    13201 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
    13261 ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
    13321 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
    13381 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
    13441 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
    13501 catggc
//
