LOCUS       sequence-41532-e         467 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Koo BK, Stange DE, Sato T, Karthaus W, Farin HF, Huch M, van Es JH, 
            Clevers H
  TITLE     Controlled gene expression in primary Lgr5 organoid cultures.
  JOURNAL   Nat Methods. 2011 Dec 4. doi: 10.1038/nmeth.1802.
  PUBMED    22138822
REFERENCE   2  (bases 1 to 467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2011 Dec 4. doi: 10.1038/nmeth.1802."
COMMENT     Sequence Label: pMSCV-loxp-dsRed-loxp-3xHA-Puro-WPRE
FEATURES             Location/Qualifiers
     source          1..467
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(176..196)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 attttgtaat ccagaggttg attatcggga attatcgatg catggggtcg tgcgctcctt
       61 tcggtcgggc gctgcgggtc gtggggcggg cgtcaggcac cgggcttgcg ggtcatgcac
      121 caggtgcgcg gtccttcggg cacctcgacg tcggcggtga cggtgaagcc gagccgctcg
      181 tagaagggga ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg
      241 gccgcctcca ctccggggag cacgacggcg ctgcccagac ccttgccctg gtggtcgggc
      301 gagacgccga cggtggccag gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg
      361 ccttccatct gttgctgcgc ggccagccgg gaaccgctca actcggccat gctcgggccg
      421 atctcggcga acaccgcccc cgcttcaaca ctctccggcg tggtcna
//
