LOCUS       sequence-415326-        4742 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template for hlpA-YFP amplification associated with erythromycin 
            resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Mignolet J, Staub J, van der Meer JR, Veening J-W, Libante V
  TITLE     A Modular Genetic Toolbox for Precise Gene Regulation and 
            Multi-Color Imaging in Streptococci
  JOURNAL   bioRxiv 2025.05.10.653270
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.05.10.653270"
COMMENT     Sequence Label: pJethlpAYFPEry
FEATURES             Location/Qualifiers
     source          1..4742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     305..324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(2574..2580,2581..2598,2599..2604))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    2619..2640
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2760..2777)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2931..3519)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3011..3030)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3690..4481,4482..4550))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4313..4332
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4551..4655)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4723..4741
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tcatggcaaa caaacaagat ttgatcgcta aagtagcaga agctacagaa
      421 ttgactaaga aagactcagc agcagcagtt gaagctgtat ttgcagcagt agctgactat
      481 cttgcagctg gtgaaaaagt tcaattgatc ggttttggta actttgaagt tcgtgagcgt
      541 gcagaacgta aaggtcgcaa cccacaaact ggtaaagaaa tgacaattgc agcttctaaa
      601 gtaccagcat tcaaagctgg taaagctctt aaagacgctg ttaaaggatc cggatctggt
      661 ggagaagctg cagctaaatc tagttctaga ggatctggtg gagaagctgc agctaaagct
      721 ggatcaaaag gagaagaact ttttacaggt gtagtaccta tcttggttga attggatggt
      781 gatgttaacg gtcacaaatt ttctgtacgt ggtgaaggtg aaggtgatgc aactaacggt
      841 aaattgacac ttaaattgat ttgtacaact ggaaaacttc ctgttccttg gcctactctt
      901 gttacaacat tgggttatgg acttatgtgt tttgctcgtt atcctgatca tatgaaacgt
      961 cacgattttt ttaaatctgc tatgccagaa ggttatgtac aagaacgtac aatttcattt
     1021 aaagatgacg gaacatataa aacacgtgct gaagtaaaat tcgaaggtga cactcttgtt
     1081 aatcgtatcg aattgaaagg aatcgatttc aaagaagatg gtaacatttt gggacacaaa
     1141 cttgaataca acttcaactc tcataatgtt tatatcacag ctgacaaaca aaaaaacggt
     1201 attaaagcta attttaaaat tcgtcacaat gttgaagatg gaggagttca attggctgat
     1261 cattatcaac aaaatacacc aatcggagac ggaccagtat tgcttccaga taaccactac
     1321 ctttcttatc aatcaaaact ttcaaaagat cctaacgaaa aacgtgacca tatggtactt
     1381 cttgaatttg ttacagcagc aggtatcact cacggtatgg acgaacttta taaataagaa
     1441 ttccaaactt aagagtgtgt tgatagtgca gtatcttaaa attttgtata ataggaattg
     1501 aagttaaatt agatgctaaa aatttgtaat taagaaggag tgattacatg aacaaaaata
     1561 taaaatattc tcaaaacttt ttaacgagtg aaaaagtact caaccaaata ataaaacaat
     1621 tgaatttaaa agaaaccgat accgtttacg aaattggaac aggtaaaggg catttaacga
     1681 cgaaactggc taaaataagt aaacaggtaa cgtctattga attagacagt catctattca
     1741 acttatcgtc agaaaaatta aaactgaata ctcgtgtcac tttaattcac caagatattc
     1801 tacagtttca attccctaac aaacagaggt ataaaattgt tgggagtatt ccttaccatt
     1861 taagcacaca aattattaaa aaagtggttt ttgaaagcca tgcgtctgac atctatctga
     1921 ttgttgaaga aggattctac aagcgtacct tggatattca ccgaacacta gggttgctct
     1981 tgcacactca agtctcgatt cagcaattgc ttaagctgcc agcggaatgc tttcatccta
     2041 aaccaaaagt aaacagtgtc ttaataaaac ttacccgcca taccacagat gttccagata
     2101 aatattggaa gctatatacg tactttgttt caaaatgggt caatcgagaa tatcgtcaac
     2161 tgtttactaa aaatcagttt catcaagcaa tgaaacacgc caaagtaaac aatttaagta
     2221 ccgttactta tgagcaagta ttgtctattt ttaatagtta tctattattt aacgggagga
     2281 aataattcta tgagtcgctt ttgtaaattt ggaaagttac acgttactaa agggaatgta
     2341 gataaattat taggtatact actgacagct tccaaggagc taaagaggtc caagcttcag
     2401 gaaactgaga caggaatttt attaaaaatt taaattttga agaaagttca gggttaatag
     2461 catccatttt ttgctttgca agttcctcag cattcttaac aaaagacgtc tcttttgaca
     2521 tgtttaaagt ttaaacctcc tgtgtgaaat tattatccgc tcataattcc acacattata
     2581 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     2641 attgcgttgc gctcactgcc aattgctttc cagtcgggaa acctgtcgtg ccagctgcat
     2701 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     2761 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2821 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2881 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2941 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     3001 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     3061 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3121 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3181 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3241 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3301 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3361 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3421 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3481 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3541 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3601 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3661 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3721 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3781 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3841 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3901 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3961 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     4021 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     4081 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4141 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4201 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     4261 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     4321 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4381 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4441 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4501 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4561 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4621 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     4681 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     4741 cc
//
