LOCUS       sequence-415327-        6076 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Truncated version of human Tyrosinase as control virus for 
            Tyrosinase expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6076)
  TITLE     Prigge Lab AAV vectors
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV_Cbh_DIO_hTyrNull_HATag
FEATURES             Location/Qualifiers
     source          1..6076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        145..430
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          655..882
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     protein_bind    complement(912..945)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(996..1029)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1041..1067)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(2697..2723)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    2744..2777
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    2828..2861
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2931..2951)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     repeat_region   3339..3479
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3339..3468
                     /label=AAV2 ITR
     rep_origin      3554..4009
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3641..3660)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3851..3872
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4026..4045)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4145..4167
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4205..4223)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4291..4395
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4396..4464,4465..5256)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4614..4633)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5427..6015
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5916..5935
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgttac ataacttacg gtaaatggcc cgcctggctg
      181 accgcccaac gacccccgcc cattgacgtc aatagtaacg ccaataggga ctttccattg
      241 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      301 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattgtgc
      361 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      421 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
      481 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
      541 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
      601 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
      661 gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
      721 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
      781 tgtaattagc tgagcaagag gtaagggttt aagggatggt tggttggtgg ggtattaatg
      841 tttaattacc tggagcacct gcctgaaatc actttttttc aggttgggga tcctctagag
      901 tcgactccgg aataacttcg tataggatac tttatacgaa gttatgcaga atggtagctg
      961 gattgtagct gctattagca atatgaaacc tcttaataac ttcgtatagc atacattata
     1021 cgaagttatg gcgcgcctta agcgtaatct ggaacatcgt atgggtaaaa ctccccggac
     1081 ccagctgcta aatggctctg atacaagctg tggtaatcct ctttctccat gaggagtggc
     1141 tgcttttctt caggaagctg ctttctcttg tgacgacaca gcaagctcac aagccctgcc
     1201 agcagggcag tgaggacggc ccctaccatc gccgccccaa ggagccatga ccagatccga
     1261 ctcgcttgtt ccaaatagga cttaatgtag tcttgaaaag agtctgggtc tgaatcttgt
     1321 agatagctat agtcatagcc cagatctttg gatgaaataa agaaatcacc atttctgtac
     1381 agtggtataa aaggaaccat gtaggattcc cggttatgtc caatgggtgc attggcttct
     1441 ggataaactt cttgaagagg acggtgcctt cggagccact gctcaaaaat actgtcaaca
     1501 aatgcatggt gaagaaggaa gataggatcg ttggcagatc cctgtacctg ggacattgtt
     1561 ccattcatat agatgtgcaa ggcattgtgc atgctgcttt gagaggcatc cgctatccca
     1621 gtaagtggac tagcaaatcc ttccagtgta tttctaaagc tgaaattggc agctttatcc
     1681 atggaaccag attcatattg ggtcaaactc aggcaaaatt ctacatcagc tgaagagggg
     1741 agccttgggg ttctggattt gtcatggttt ccaggattac gccgtaaagg tccctcgggc
     1801 gttccattgc ataaagactg atggctgttg tactcctcca atcggctaca gacaatctgc
     1861 caagaggaga agaatgatgc tgggctgagt aagttaggat ttgtggggtg ctgacctccc
     1921 atgtactcat ctgtgcaaat gtcacacttt tctgcatccc gccagtccca atatggaata
     1981 gtgaagtttt catctcctgt cagcttctgg atttcttgtt cccaccgcaa caagaagagt
     2041 ctatgccaag gcagaaaagc tggtgcttca tgggcaaaat caatgtctct ccagatttca
     2101 gatcccccaa gcagtgcatc cattgacaca taataatgtt acatccagac aaagaggtca
     2161 taaatattga tgtcgttaaa catgggtgtt gatccatttt tcatttggcc ataggtccct
     2221 atggggatga catagtctga gctgatggta tgctttgcta aagtgaggta ggcaaaaaat
     2281 ttgtccttct ctggggcact caaatcgaag atgtttcttc tcaccaagag tcgtctctct
     2341 gtgcagtttg gtccccaaaa gccaaacttg cagtttccac agttgaatcc catgaagttg
     2401 ccagagcact ggcaggtcct attataaaag acggaaggcc acgactcccg gtcatccacc
     2461 cctgtgaagg gaaattgagg cccaagtggt gcattggaca gaaggatatt ctgacaggaa
     2521 cctctgcctg aaagctggcc acagggactc ctgtccccgc tccacggtgg acagcattcc
     2581 ttctccatca ggttcttaga ggagacacag gctctaggga aatggccagc ggaggtctgg
     2641 aaactccaca gcaggcagta caaaacagcc aggagaaact ccccggaccc agctgcagcg
     2701 taatctggaa catcgtatgg gtacatcaag gtcttaggct agcataactt cgtataaagt
     2761 atcctatacg aagttatttg ccttaaccca gaaattatca ctgttattct ttagaatggt
     2821 gcaaagaata acttcgtata atgtatgcta tacgaagtta tgaattccgc tcgagataat
     2881 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     2941 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3001 gctttcattt tctcctcctt gtataaatcc tggttagttc ttgccacggc ggaactcatc
     3061 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     3121 gtgtttattt gtgaaatttg tgatgctatt gctttatttg taaccatcta gctttatttg
     3181 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     3241 caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta
     3301 aagcggccgc ggtaaccacg tgcggaccga gcggccgcag gaacccctag tgatggagtt
     3361 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     3421 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
     3481 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     3541 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     3601 cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct
     3661 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     3721 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     3781 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     3841 ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat
     3901 tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt
     3961 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
     4021 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     4081 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     4141 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     4201 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     4261 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4321 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4381 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4441 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4501 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4561 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4621 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     4681 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4741 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4801 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4861 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4921 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4981 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     5041 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     5101 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     5161 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5221 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5281 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5341 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5401 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5461 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5521 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     5581 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5641 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5701 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5761 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5821 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5881 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5941 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6001 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6061 ccttttgctc acatgt
//
