LOCUS       sequence-415331-        6564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Fujita N, Jaye DL, Kajita M, Geigerman C, Moreno CS, Wade PA
  TITLE     MTA3, a Mi-2/NuRD complex subunit, regulates an invasive growth 
            pathway in breast cancer.
  JOURNAL   Cell. 2003 Apr 18. 113(2):207-19.
  PUBMED    12705869
REFERENCE   2  (bases 1 to 6564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2003 
            Apr 18. 113(2):207-19."
COMMENT     Sequence Label: Snail_pGL2
FEATURES             Location/Qualifiers
     source          1..6564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1..122
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             1189..2841
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(1255..1273)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          3082..3147
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3277..3297
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3722..3856
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3998..4015)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4169..4757)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4249..4268)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4928..5719,5720..5788))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5551..5570
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5789..5893)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5920..6375
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6007..6026)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6217..6238
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
       61 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
      121 tatggtactg taactgagct aacataaccc gggaggtacc tctagactga aaatccttcg
      181 gtggctcccc agtgcccttg aggcaaagtc caaactccta cgaggccctg gggcccagca
      241 catctgaccc ctccgggcat gcccatccca ccccatccct ggaagctgct ctctaggagt
      301 tactctgaag cagttgccac ttcttccctc gggccttttc ccttgataat tcttcacttc
      361 ctctgggaag tcaccccgac cccctgtcag gtgacccgcc tcttaacggt cgccgcgtcc
      421 cgtctctccc ccaccaaagc acacttccct ttgcattgta attatctgtt tacttcgtct
      481 gtctccctca ctggaccaga agctaccctt cgggagaggc tctgagtgtt ctgtccgggg
      541 ctgtgccctg gccccaggta cagtgcccca cacgtgctgg gcgctccgta aacactggat
      601 aagggaagga acgggtgctc ttggctagct gggccaggct gctttgcaaa aaggccgtgg
      661 catttcaagt cgccgagagc cacgtgcggt gtccctttcc tcgcttcctc cccagcgatg
      721 tgcgtttccc tcgtcaatgc cacgctctcc aggcgccagc cgggcggagg aaatttccgc
      781 cccctcccaa gcccgaggcg ggggcgggcg tcggaaggtc aggtgtcccg gccggcgcgc
      841 agcgccaggg ggcgtcagaa gcgctcagac caccgggcgc tgagccggtg ggcgcgcggc
      901 gtcctgccgg ggtcccacct cgcaggggcc tcgcttcgct cgacgtcccg ccccggacag
      961 ccccagcgcg ggggacgacc cgcgctgcgc cagcgaaccc cgcctcggag gagtccccgc
     1021 ccgggctctc accgccacgc ggcgcgagcc cggccagcag ccggcgcacc tgctcgggga
     1081 gtggccttcg gcggagacga gcctccgatt ggcgcgaagg tgacaaaggg gcgtggcaga
     1141 taaggccccg cccctcgaga agcttggcat tccggtactg ttggtaaaat ggaagacgcc
     1201 aaaaacataa agaaaggccc ggcgccattc tatcctctag aggatggaac cgctggagag
     1261 caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat
     1321 gcacatatcg aggtgaacat cacgtacgcg gaatacttcg aaatgtccgt tcggttggca
     1381 gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac
     1441 tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc
     1501 gcgaacgaca tttataatga acgtgaattg ctcaacagta tgaacatttc gcagcctacc
     1561 gtagtgtttg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaaattacca
     1621 ataatccaga aaattattat catggattct aaaacggatt accagggatt tcagtcgatg
     1681 tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt tgtaccagag
     1741 tcctttgatc gtgacaaaac aattgcactg ataatgaatt cctctggatc tactgggtta
     1801 cctaagggtg tggcccttcc gcatagaact gcctgcgtca gattctcgca tgccagagat
     1861 cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat
     1921 cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta
     1981 atgtatagat ttgaagaaga gctgttttta cgatcccttc aggattacaa aattcaaagt
     2041 gcgttgctag taccaaccct attttcattc ttcgccaaaa gcactctgat tgacaaatac
     2101 gatttatcta atttacacga aattgcttct gggggcgcac ctctttcgaa agaagtcggg
     2161 gaagcggttg caaaacgctt ccatcttcca gggatacgac aaggatatgg gctcactgag
     2221 actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa
     2281 gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt
     2341 aatcagagag gcgaattatg tgtcagagga cctatgatta tgtccggtta tgtaaacaat
     2401 ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct
     2461 tactgggacg aagacgaaca cttcttcata gttgaccgct tgaagtcttt aattaaatac
     2521 aaaggatatc aggtggcccc cgctgaattg gaatcgatat tgttacaaca ccccaacatc
     2581 ttcgacgcgg gcgtggcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt
     2641 gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt
     2701 caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa
     2761 ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag
     2821 ggcggaaagt ccaaattgta aaatgtaact gtattcagcg atgacgaaat tcttagctat
     2881 tgtaatactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattggtgcc
     2941 cttaaacgcc tggttgctac gcctgaataa gtgataataa gcggatgaat ggcagaaatt
     3001 cgccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
     3061 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
     3121 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
     3181 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
     3241 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
     3301 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
     3361 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
     3421 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
     3481 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
     3541 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
     3601 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     3661 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     3721 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     3781 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     3841 ttatcatgtc tggatccgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
     3901 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
     3961 actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac tcgctgcgct
     4021 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     4081 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     4141 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     4201 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     4261 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     4321 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     4381 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     4441 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     4501 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     4561 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     4621 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     4681 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     4741 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4801 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4861 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     4921 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4981 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     5041 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     5101 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     5161 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     5221 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     5281 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     5341 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     5401 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     5461 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     5521 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     5581 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5641 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5701 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5761 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5821 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5881 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
     5941 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     6001 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     6061 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6121 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     6181 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     6241 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     6301 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     6361 taacgcttac aatttgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     6421 tgcgggcctc ttcgctatta cgccagccca agctaccatg ataagtaagt aatattaagg
     6481 tacgtggagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     6541 aaaatgaatg caattgttgt tgtt
//
