LOCUS       sequence-415340-       12061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Premade plasma membrane marker, 
            pUBQ10:PiP1;4:mTurquoise2:tHSP18.2M:FastRed, for braiding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12061)
  AUTHORS   Piepers M, Erbstein K, Reyes-Hernandez J, Song C, Tessi T, 
            Petrasiunaite V, Faerber N, Distel K, Maizel A
  TITLE     GreenGate 2.0: Backwards compatible addons for assembly of complex 
            transcriptional units and their stacking with GreenGate.
  JOURNAL   PLoS One. 2023 Sep 8;18(9):e0290097. doi: 
            10.1371/journal.pone.0290097. eCollection 2023.
  PUBMED    37682951
REFERENCE   2  (bases 1 to 12061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2023-09-8"; volume: "18(9):e0290097. doi"; pages: " 
            10.1371/journal.pone.0290097. eCollection 2023"
COMMENT     Sequence Label: pKD016
FEATURES             Location/Qualifiers
     source          1..12061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1611..1613,1614..1616,1617..2330)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1656..1677)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1917..1936)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2264..2285
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4742..5449
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MLSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     5155..5174
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5726..5750
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7051..7680
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8109..9182
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9248..9442
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9678..9700)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9786..9926
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9841..9860
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9941..9958)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10112..10700)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10192..10211)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10788..11603)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(10788..11603)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     11511..11530
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    12036..12060
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 taacgaatta ccatggcacc tgctgcaacc tcgacgagtc agtaataaac ggcgtcaaag
       61 tggttgcagc cggcacacac gagtcgtgtt tatcaactca aagcacaaat acttttcctc
      121 aacctaaaaa taaggcaatt agccaaaaac aactttgcgt gtaaacaacg ctcaatacac
      181 gtgtcatttt attattagct attgcttcac cgccttagct ttctcgtgac ctagtcgtcc
      241 tcgtcttttc ttcttcttct tctataaaac aatacccaaa gagctcttct tcttcacaat
      301 tcagatttca atttctcaaa atcttaaaaa ctttctctca attctctcta ccgtgatcaa
      361 ggtaaatttc tgtgttcctt attctctcaa aatcttcgat tttgttttcg ttcgatccca
      421 atttcgtata tgttctttgg tttagattct gttaatctta gatcgaagac gattttctgg
      481 gtttgatcgt tagatatcat cttaattctc gattagggtt tcatagatat catccgattt
      541 gttcaaataa tttgagtttt gtcgaataat tactcttcga tttgtgattt ctatctagat
      601 ctggtgttag tttctagttt gtgcgatcga atttgtagat taatctgagt ttttctgatt
      661 aacaaacagt attcagtcga ctggtaccaa caggctcaac aatggaaggc aaagaagaag
      721 atgtacgagt gggagctaac aagttcccgg agaggcaacc catcggtaca tcggctcagt
      781 ccaccgacaa ggactacaaa gagccacctc ctgcgccact gttcgagccc ggcgagctca
      841 gctcatggtc tttctacaga gccggaatag ctgagtttat cgctactttc ttgtttctct
      901 acatcactgt tttgactgta atgggagtta agagagcacc aaacatgtgt gcttctgttg
      961 gaatccaagg tatcgcgtgg gcttttggtg gcatgatctt tgctcttgtc tactgtactg
     1021 ctggaatttc aggtggacac atcaaccctg ctgtaacatt cggtctgttc ttggctcgga
     1081 agttatctct gaccagagca gtgttctaca tgattatgca atgccttgga gccatctgtg
     1141 gtgccggagt cgtcaaaggt ttccagccaa cgccgtacca gactctcggt ggtggtgcta
     1201 acaccgttgc tcatggctac accaaaggtt ctggccttgg tgctgaaatc atcggaacat
     1261 tcgttctcgt ctacactgtc ttctccgcca ccgacgccaa gagaagcgcc cgtgactcac
     1321 acgtcccgat tttggcgccg ctcccaatcg gatttgcagt gttcttggta cacttggcaa
     1381 caataccgat caccgggacc ggaatcaacc cagctagaag tcttggagcc gcaattatct
     1441 acaacaagga ccactcttgg gatgaccatt ggattttctg ggttggacca ttcattggag
     1501 cagctctagc agcactatat caccagattg tcatcagagc gattcctttc aagagcaaga
     1561 gttcaggtgc tagcggtggc agcggtggca ctagtggtgg aggcggatcc atggtgagca
     1621 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1681 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1741 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1801 ccctgtcctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     1861 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1921 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1981 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     2041 acaactactt tagcgacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
     2101 ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc
     2161 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     2221 cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     2281 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtag ctgcgagctc
     2341 atatgaagat gaagatgaaa tatttggtgt gtcaaataaa tagcttgtgt gcttaagttt
     2401 gtgttttttt cttggcttgt tgtgttatga atttgtggct ttttctaata ttaaatgaat
     2461 gtaagatctc attataatga ataaacaaat gtttctataa tccattgtga atgttttgtt
     2521 ggatctcttc tgcagcatat aactactgta tgtgctatgg tatggactat ggaatatgat
     2581 taaagataag gaattcacta cttcaagtgt atgtaggtat agtaacatga acaagaacag
     2641 actcaagtac aagatcgcat acgaaaatgg aaatggcaat gtcacttcca cataatcaaa
     2701 cacgaatcct catatcaaca aggcctgaga ttctaactag ctcataacaa cttagccaat
     2761 agttacttga gactaccaaa tgtatgtaga actaaagact aagggacaga gagttcgtct
     2821 aaacaggtga atctagtcgt tgttatctaa taaacaattc agccccaaat gcagaacaca
     2881 catagagctc tctattgatt caaattacga tctgatactg ataacgtcta gatttttagg
     2941 gttaaagcaa tcaatcacct gacgattcaa ggtggttgga tcatgacgat tccagaaaac
     3001 atcaagcaag ctctcaaagc tacactcttt gggatcatac tgaactctaa caacctcgtt
     3061 atgtcccgta gtgccagtac agacatcctc gtaactcgga ttgtgcacga tgccatggct
     3121 atacccaacc tcggtcttgg tcacaccagg aactctctgg taagctagct ccactcccca
     3181 gaaacaaccg gcgccaaatt gcgcgaattg ctgacctgaa gacggaacat catcgtcggg
     3241 tccttgggcg attgcggcgg aagatgggtc agcttgggct tgaggaccag acccgaatcc
     3301 gagtctgttg aaaaggttgt tcattgggga tttgtatacg gagattggtc gtcgagaggt
     3361 ttgagggaaa ggacaaatgg gtttggctct ggagaaagag agtgcggctt tagagagaga
     3421 attgagaggt ttagagagag atgcggcggc gatgagcgga ggagagacga cgaggacctg
     3481 cattatcaaa gcagtgacgt ggtgaaattt ggaactttta agaggcagat agatttatta
     3541 tttgtatcca ttttcttcat tgttctagaa tgtcgcggaa caaattttaa aactaaatcc
     3601 taaatttttc taattttgtt gccaatagtg gatatgtggg ccgtatagaa ggaatctatt
     3661 gaaggcccaa acccatactg acgagcccaa aggttcgttt tgcgttttat gtttcggttc
     3721 gatgccaacg ccacattctg agctaggcaa aaaacaaacg tgtctttgaa tagactcctc
     3781 tcgttaacac atgcagcggc tgcatggtga cgccattaac acgtggccta caattgcatg
     3841 atgtctccat tgacacgtga cttctcgtct cctttcttaa tatatctaac aaacactcct
     3901 acctcttcca aaatatatac acatcttttt gatcaatctc tcattcaaaa tctcattctc
     3961 tctagtaaac aagaacaaaa aaatggcgga tacagctaga ggaacccatc acgatatcat
     4021 cggcagggac cagtacccga tgatgggccg agatcgagat cagtaccaga tgtccggacg
     4081 aggatctgac tactccaagt ctaggcagat tgctaaagct gcaactgctg tcacagctgg
     4141 tggttccctc cttgttctct ccagccttac ccttgttgga actgtcatag ctttgactgt
     4201 tgcaacacct ctgctcgtta tcttcagccc aatccttgtc ccggctctca tcacagttgc
     4261 actcctcatc accggttttc tttcctctgg agggtttggc attgccgcta taaccgtttt
     4321 ctcttggatt tacaagtaag cacacattta tcatcttact tcataatttt gtgcaatatg
     4381 tgcatgcatg tgttgagcca gtagctttgg atcaattttt ttggtcgaat aacaaatgta
     4441 acaataagaa attgcaaatt ctagggaaca tttggttaac taaatacgaa atttgaccta
     4501 gctagcttga atgtgtctgt gtatatcatc tatataggta aaatgcttgg tatgatacct
     4561 attgattgtg aataggtacg caacgggaga gcacccacag ggatcagaca agttggacag
     4621 tgcaaggatg aagttgggaa gcaaagctca ggatctgaaa gacagagctc agtactacgg
     4681 acagcaacat actggtgggg aacatgaccg tgaccgtact cgtggtggcc agcacactac
     4741 tatgttgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     4801 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     4861 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     4921 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     4981 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     5041 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     5101 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     5161 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     5221 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     5281 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     5341 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     5401 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagc atatgtgagt
     5461 atctagagtc aagcagatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
     5521 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
     5581 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
     5641 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaagtatgt
     5701 catatggcag gtgctcgagc aattctgaca ggatatattg gcgggtaaac ctaagagaaa
     5761 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
     5821 catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac
     5881 ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtca tctgaaaacg
     5941 acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt
     6001 ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt
     6061 acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg
     6121 accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt
     6181 ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc
     6241 tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg
     6301 acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag
     6361 agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca
     6421 ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca
     6481 aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg
     6541 cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg
     6601 tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg
     6661 ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg
     6721 ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc
     6781 gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc
     6841 gcccgcgcac ctctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa
     6901 gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag
     6961 gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg
     7021 agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa
     7081 ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg
     7141 gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg
     7201 cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg
     7261 aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg
     7321 gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac
     7381 gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat
     7441 ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt
     7501 gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag
     7561 cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag
     7621 ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga
     7681 gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga
     7741 gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc
     7801 gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc
     7861 aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga
     7921 gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa
     7981 gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca
     8041 ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca ctggaacccc caagcccgag
     8101 gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg
     8161 atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag
     8221 aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc
     8281 aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag
     8341 attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg
     8401 tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc
     8461 ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt
     8521 acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag
     8581 ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct
     8641 gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa
     8701 acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg
     8761 tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc
     8821 cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga
     8881 acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt
     8941 ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga
     9001 cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca
     9061 agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg
     9121 gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct
     9181 aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggac
     9241 tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc
     9301 cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata
     9361 taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta
     9421 aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag
     9481 cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg
     9541 ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag
     9601 ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc
     9661 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg tgacggtcac agcttgtctg
     9721 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     9781 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
     9841 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     9901 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     9961 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    10021 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    10081 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    10141 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    10201 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    10261 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    10321 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    10381 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    10441 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    10501 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
    10561 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    10621 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    10681 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    10741 ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc taggtgatta gaaaaactca
    10801 tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga
    10861 aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga
    10921 tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc
    10981 tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag
    11041 aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg
    11101 tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgagt
    11161 cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc
    11221 aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc
    11281 tggaatgctg ttttccctgg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg
    11341 ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc
    11401 tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca
    11461 tcgggcttcc catacaatcg gtagattgtc gcacctgatt gcccgacatt atcgcgagcc
    11521 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct tgagcaagac
    11581 gtttcccgtt gaatatggct cataacagaa cttattattt ccttcctctt ttctacagta
    11641 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc
    11701 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta
    11761 taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg
    11821 tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc
    11881 ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg
    11941 gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg
    12001 ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac
    12061 a
//
