LOCUS       sequence-415371-        3041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTR4 plasmid with CDS of Arabidopsis thaliana MEMBRANE-ASSOCIATED 
            MANNITOL-INDUCED (MAMI, AT5G54110.1) including stop codon for 
            N-terminal epitope tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Simranjit Kaur, Thomas Colby, Domenika Thieme, Carsten Proksch, 
            Susanne Matschi, eIvan Matic,, Lennart Wirthmueller
  TITLE     Untargeted proteomics identifies plant substrates of the 
            bacterial-derived ADP-ribosyltransferase AvrRpm1
  JOURNAL   bioRxiv 2023.09.25.558804
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.09.25.558804"
COMMENT     Sequence Label: pENTR4 CDS A. thaliana MAMI
FEATURES             Location/Qualifiers
     source          1..3041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     500..519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      764..850
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      942..969
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     977..1000
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    1019..1118
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      1251..1260
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1931..2030)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2142..2161)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2153..2962
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2220..2239)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
       61 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      121 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      181 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      241 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      301 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      361 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      421 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      481 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      541 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      601 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
      661 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgctagc atggatctcg
      721 gggacgtcta actactaagc gagagtaggg aactgccagg catcaaataa aacgaaaggc
      781 tcagtcggaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
      841 taggacaaat ccgccgggag cggatttgaa cgttgtgaag caacggcccg gagggtggcg
      901 ggcaggacgc ccgccataaa ctgccaggca tcaaactaag cagaaggcca tcctgacgga
      961 tggccttttt gcgtttctac aaactcttcc tgttagttag ttacttaagc tcgggcccca
     1021 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg
     1081 cttttttata atgccaactt tgtacaaaaa agcaggctcc actccaatag gtgaccgtca
     1141 gaatccgagt gtggagaaga agaagaacct cttcaggcta tgtccgtttt ggcagaggag
     1201 aagcaccact tcttcatctt caacacagaa tccaaaccaa aactaccgta gccgccatgg
     1261 aaatcggaat acagatattt ccgccgtctc taagcctccg ttgactatgt cttccgtcgc
     1321 cagatcactt cttccagctc gccgtcgtct ccgactagat ccttctagtt atctgtattt
     1381 cccttatgaa cctggaaaac aagttcgcag cgcaataaag cttaagaaca ctagcaaatc
     1441 tcatactgca tttaagttcc aaacaacggc acctaagagt tgttatatgc gtcctcccgg
     1501 aggtgttttg gctccaggag agagtgtttt tgctactgtg ttcaagtttg tggaacatcc
     1561 agagaacaat gaaaagcagc cgttgaatca gaagagcaag gttaagttta agattatgag
     1621 cctgaaagtt aaaccaggag tagagtatgt gcctgagttg tttgatgagc aaaaggatca
     1681 agtagcagtg gagcaggttc ttcgggtcat ttttattgat gcggaccgcc caagtgctgc
     1741 attggagaaa ctgaaacgtc aattggatga agctgaagct gcagttgaag caagaaagaa
     1801 gcctccccca gagacagggc ctcgagttgt cggggagggt cttgtcatag acgaatggaa
     1861 ggagagaaga gagaagtacc ttgctcggca acaagtcgaa tccgtagatt ctttgtccta
     1921 agatatctag acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     1981 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     2041 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     2101 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
     2161 tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg
     2221 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg
     2281 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     2341 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     2401 gcattttatc cgtactcctg gtgatgcatg gttactcacc actgcgatcc ccggaaaaac
     2461 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     2521 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     2581 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     2641 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact
     2701 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     2761 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     2821 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     2881 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     2941 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacatt attcagattg
     3001 ggccccgttc cactgagcgt cagaccccgt agaaaagatc a
//
