LOCUS       sequence-415372-        5237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTR4 plasmid with CDS of Arabidopsis thaliana PHOTOTROPIN 1 
            (PHOT1, AT3G45780.1) without stop codon for C-terminal epitope tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Simranjit Kaur, Thomas Colby, Domenika Thieme, Carsten Proksch, 
            Susanne Matschi, eIvan Matic,, Lennart Wirthmueller
  TITLE     Untargeted proteomics identifies plant substrates of the 
            bacterial-derived ADP-ribosyltransferase AvrRpm1
  JOURNAL   bioRxiv 2023.09.25.558804
REFERENCE   2  (bases 1 to 5237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.09.25.558804"
COMMENT     Sequence Label: pENTR4 CDS A. thaliana PHOT1
FEATURES             Location/Qualifiers
     source          1..5237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     500..519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      764..850
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      942..969
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     977..1000
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    1019..1118
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1278..1295)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     protein_bind    complement(4127..4226)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4338..4357)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4349..5158
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4416..4435)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
       61 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      121 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      181 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      241 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      301 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      361 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      421 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      481 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      541 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      601 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
      661 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgctagc atggatctcg
      721 gggacgtcta actactaagc gagagtaggg aactgccagg catcaaataa aacgaaaggc
      781 tcagtcggaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
      841 taggacaaat ccgccgggag cggatttgaa cgttgtgaag caacggcccg gagggtggcg
      901 ggcaggacgc ccgccataaa ctgccaggca tcaaactaag cagaaggcca tcctgacgga
      961 tggccttttt gcgtttctac aaactcttcc tgttagttag ttacttaagc tcgggcccca
     1021 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg
     1081 cttttttata atgccaactt tgtacaaaaa agcaggctcc acatggaacc aacagaaaaa
     1141 ccatcgacca aaccatcttc tcggactcta cctagagaca ctcgtggctc tctcgaagta
     1201 ttcaacccgt caactcagct gacccgaccc gataacccgg tgttccgtcc tgaaccacca
     1261 gcgtggcaaa acttgagtga tccacgtggc accagtcctc aaccccgacc acaacaagaa
     1321 ccagctccat ccaaccctgt tcggtctgat caagaaatcg ctgtcacgac ctcatggatg
     1381 gctctgaaag acccatcacc ggagacaatc tccaagaaga cgatcaccgc cgagaaacca
     1441 cagaagtcgg cggtggcggc tgagcagaga gcggcagagt ggggattggt tttgaagacc
     1501 gatacaaaga cgggaaagcc acaaggagta ggcgtgagga actctggtgg aactgagaat
     1561 gatcctaacg ggaaaaagac tacatcgcag agaaactcgc aaaactcttg ccggagctcc
     1621 ggtgagatgt cggacggaga tgttcccggc gggaggagtg ggattccaag agtatcggaa
     1681 gatctaaaag atgcgttgtc gacgtttcaa caaacgtttg tggtctcaga tgctacaaaa
     1741 cctgattatc cgattatgta tgcaagtgct ggtttcttca atatgactgg ttacacttcc
     1801 aaagaagtcg tcggcagaaa ctgccgattt ttacaaggat caggtacaga tgcagatgag
     1861 ttagcaaaga taagagagac attagctgct ggtaacaatt attgtgggcg tatattgaat
     1921 tacaagaaag atgggacctc tttttggaat cttctcacga ttgctcccat taaagacgag
     1981 agtggcaaag tcctcaaatt tatcggaatg caagtggagg tgagcaagca cactgaaggg
     2041 gccaaagaaa aggctttgag gcccaatggg cttccggaat ctctgattcg atacgatgcc
     2101 cgccaaaaag atatggcgac aaactcagtg acagagctag tagaggcggt gaagagacct
     2161 cgggcactaa gcgaatcgac gaatctccat cccttcatga caaaatcgga gagcgatgaa
     2221 cttccgaaaa aaccagctcg gcgaatgtcc gaaaatgttg ttccgtcagg ccgaagaaac
     2281 tctggcggcg gaagaagaaa ctcgatgcag cgaatcaacg aaattcccga gaaaaaatct
     2341 agaaaatctt ccctttcctt catggggatc aagaagaaga gcgaatcttt ggatgaatct
     2401 atagacgatg gatttataga gtatggagaa gaagacgatg agattagtga tagggacgag
     2461 agacctgaga gtgtggatga taaagtgaga caaaaggaaa tgagaaaggg tattgatcta
     2521 gctactacac tcgaacgtat cgagaagaat ttcgtcatca ctgatcctag gcttcccgat
     2581 aatcccatta tttttgcatc tgatagtttc ttggagctca cggaatatag ccgtgaagaa
     2641 attcttggaa gaaattgcag gtttctacaa ggtccagaga ctgatctaac cacagtgaag
     2701 aagattcgaa atgctattga taaccaaacc gaagtgacag ttcagctcat caactacacc
     2761 aagagcggaa agaagttctg gaacattttc cacttgcaac ctatgcgtga tcagaaggga
     2821 gaagtacaat actttattgg agttcaacta gacgggagca agcacgtaga accagttcgc
     2881 aatgtcattg aagaaaccgc agtgaaagag ggagaagacc tggtgaaaaa aacagctgtg
     2941 aatatcgatg aagcggttcg agaacttcct gatgccaaca tgacaccaga ggatttatgg
     3001 gcaaaccact caaaggttgt tcattgtaag cctcacagga aagattcacc accatggata
     3061 gctatccaaa aggtgttgga aagtggtgaa ccgattggtt tgaagcattt caaaccggtg
     3121 aaacctttgg gttctggtga cacaggaagt gtgcatctag tggaactcgt tggaacagat
     3181 cagttgtttg caatgaaagc aatggacaag gctgttatgc ttaaccgaaa caaagtacat
     3241 agagctagag ctgagagaga gatcctagat ttgcttgacc atccttttct tcctgcactc
     3301 tacgcttctt ttcagacaaa aacacatata tgtcttataa cagattacta tccaggagga
     3361 gaactcttca tgctcctaga tcgacaacct aggaaggttc tcaaagaaga tgctgtaaga
     3421 ttctatgctg ctcaagttgt cgttgcactc gagtatcttc actgtcaagg aataatttac
     3481 cgagacttga aaccggaaaa cgttttaatc caaggcaatg gcgatatctc tttgtcggat
     3541 tttgatctgt cttgcttgac atcttgcaaa cctcagctgt tgattccgag tatagacgag
     3601 aagaagaaga aaaagcaaca aaagagtcaa caaactccaa tcttcatggc tgaaccaatg
     3661 cgtgcatcaa actcatttgt tggcactgaa gagtacattg ctccggaaat tatatccgga
     3721 gcaggacata cgagtgcagt agactggtgg gctcttggta tacttatgta tgaaatgtta
     3781 tacggataca ctccttttag aggaaaaaca agacagaaga ctttcaccaa tgttcttcaa
     3841 aaagatctca agtttccagc tagcattcct gcaagtcttc aagtgaaaca gctgattttt
     3901 aggctgttac aacgagatcc gaagaaaaga ctaggttgtt ttgaaggagc aaatgaagtc
     3961 aagcaacatt ctttcttcaa aggcataaat tgggctctga ttcgatgcac gaaccctcca
     4021 gagctcgaga ctccgatatt ttctggtgaa gctgaaaacg gagagaaagt tgttgatcct
     4081 gagctagaag atctgcaaac aaatgttttt aactcgagat atctagaccc agctttcttg
     4141 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     4201 atcagtcaaa ataaaatcat tatttgccat ccagctgcag ctctggcccg tgtctcaaaa
     4261 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc
     4321 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc
     4381 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     4441 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt
     4501 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     4561 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctggtga
     4621 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata
     4681 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     4741 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
     4801 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     4861 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt
     4921 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     4981 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     5041 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     5101 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     5161 agaattggtt aattggttgt aacattattc agattgggcc ccgttccact gagcgtcaga
     5221 ccccgtagaa aagatca
//
