LOCUS       sequence-415375-        5977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTR4 plasmid with genomic sequence of Arabidopsis thaliana 
            PHOSPHOLIPASE D GAMMA 3 (PLDGAMMA3, AT4G11840) without stop codon 
            for C-terminal epitope tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Simranjit Kaur, Thomas Colby, Domenika Thieme, Carsten Proksch, 
            Susanne Matschi, eIvan Matic,, Lennart Wirthmueller
  TITLE     Untargeted proteomics identifies plant substrates of the 
            bacterial-derived ADP-ribosyltransferase AvrRpm1
  JOURNAL   bioRxiv 2023.09.25.558804
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.09.25.558804"
COMMENT     Sequence Label: pENTR4 genomic A. thaliana PLDGAMMA3
FEATURES             Location/Qualifiers
     source          1..5977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     500..519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      764..850
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      942..969
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     977..1000
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    1019..1118
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4867..4966)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5078..5097)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             5089..5898
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5156..5175)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
       61 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      121 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      181 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      241 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      301 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      361 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      421 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      481 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      541 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      601 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
      661 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgctagc atggatctcg
      721 gggacgtcta actactaagc gagagtaggg aactgccagg catcaaataa aacgaaaggc
      781 tcagtcggaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
      841 taggacaaat ccgccgggag cggatttgaa cgttgtgaag caacggcccg gagggtggcg
      901 ggcaggacgc ccgccataaa ctgccaggca tcaaactaag cagaaggcca tcctgacgga
      961 tggccttttt gcgtttctac aaactcttcc tgttagttag ttacttaagc tcgggcccca
     1021 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg
     1081 cttttttata atgccaactt tgtacaaaaa agcaggctcc acatggcgta tcatccagtt
     1141 tataacgaga ctatgtctat gggaggaggg tcaagcaacg agtttggcca gtggcttgac
     1201 aagcaactcg tgccgtttga tacaagtagt ggctctttga gggttgaatt attacatggt
     1261 aacttagaca tttgggttaa ggaagctaaa catcttccta acatggatgg tttccataac
     1321 acccttgttg gtgggatgtt ttttgggtta ggtcggagga atcataaagt ggatggtgag
     1381 aattcttcta agatcacaag tgatccttat gttactgtct ctatctctgg tgctgtgatt
     1441 ggtagaactt ttgttattag caatagtgag aatcctgtgt ggatgcagca ttttgatgta
     1501 ccagttgctc atagtgctgc gaaagttcac tttgttgtga aagacagtga tattattgga
     1561 tcacagatca ttggagctgt tgaaatccca accgagcagt tgtgttccgg gaatagaatc
     1621 gaagggctgt ttccgatact taacagtaga ggaaagcctt gtaaacaagg tgctgtgttg
     1681 agtctgtcta ttcagtatat tccaatggaa agaatgagac tttaccaaaa gggtgttggt
     1741 tttggtgttg aatgtgtagg agttcccggt acatacttcc ctttaaggaa aggcggtagg
     1801 gttactcttt atcaggatgc tcatgttgat gacggtactc ttccgagtgt acatcttgat
     1861 ggtgggattc agtatagaca tggaaaatgc tgggaagata tggctgatgc gatacgacgg
     1921 gcaaggaggt tgatttatat cacaggttgg tcagttttcc atccggttag gctggttcgt
     1981 cgtaacaatg atccgaccca aggtacatta ggagagttgc ttaaagtcaa atctcaagaa
     2041 ggtgttagag tgttggtttt ggtgtgggat gatccaactt caaggagtct tttgggattc
     2101 agtacagtaa gaatctacat aactctgttt ttttctgatt aagacattct attattgctt
     2161 acccataaaa tcaaacatgt atatatatat atatataatt ttatggagtg cctctctgtt
     2221 ttgtttctcc attttgattt atagcttatc acttaaataa ttctatggag tgcctctctg
     2281 tttgtacaca gttttgaatg gtctaatgtg ttgttcttaa cttctcacat agtcacatat
     2341 ttggttgttt cagttgcatt tcatactttc ctctattgtt tcagttctga ccctttttct
     2401 gtttatctta ttggaaagca tgagtttgct tcaattagat aattatacta acttagctac
     2461 aatccttcta tctatttaat taaaagctgg tgttttgttg catgtagaaa ggacttatga
     2521 acacaagcga tgaggagact cgccgttttt tcaagcactc atcggtgcaa gttctccttt
     2581 gtcctagata tggagggaaa ggtcacagct tcataaaaaa atctgtaaga tgctcattag
     2641 ttacttcatt actgtgtaaa ctcttgtgtg tatagcttca gcgtctaaaa tttgagtgtc
     2701 attatgatga tcaggaagtt gaaacaatct acacgcatca tcagaaaact atgattgtag
     2761 atgctgaagc agctcagaac cgaagaaaga ttgtagcatt tgttggaggg ctagacttgt
     2821 gcaacggacg atttgatact cccaagcatc ctctcttcag gacattaaag acaatccata
     2881 aagatgactt ccataaccca aattttgtgg taatcattta agaaaaaacc tgcttggttg
     2941 caattttgga attagaatcc gctattcttg atccatgtaa attctgtttt tcttattaga
     3001 ctactgcgga tgatggacca agagaaccat ggcatgatct gcacagcaag attgatggtc
     3061 cagctgcata tgatgtgctt gctaattttg aggaacgctg gatgaaggct tcaaaacctc
     3121 gcggaattgg gagactaaga acttcttctg atgattcttt gcttaggtta gacagaattc
     3181 ctgacataat gggactttcc gaagcttctt ctgctaatga taatgatcca gagtcttggc
     3241 atgttcaggt aatttagcaa gtctaatttt tttgctagtt attgttattt gtggtggtga
     3301 caaaaagaat ctcatttttt ctccaatgat attaatcttt aggttttccg ttcaattgat
     3361 tcgagttcag ttaaagggtt tccaaaggac ccaaaggaag ctactggaag agtaagatag
     3421 caccacaact ctgagctgtt tttgtttgtc atcatgttgc tatctccgac tgattatgta
     3481 ttcatgaaca gaaccttcta tgtgggaaga acattctcat agatatgagc atacatgcag
     3541 cttatgttaa agccatacga tctgctcagc acttcattta cattgaaaac caatattttc
     3601 ttggatcatc attcaattgg gattcaaaca agaacttggg taagaaactt taaagctttt
     3661 gaaggaaagc ttagtaatcg atgcgtacaa tatgtaaaca aggagttttg gcattgcagg
     3721 tgcgaataat ctaatcccga tggaaatcgc gctgaagatt gctaacaaaa ttagagcaag
     3781 ggaaaagttt gctgcttata ttgtcattcc aatgtggcca gaaggtgctc caacaagtaa
     3841 tcctattcaa agaattctct actggcaggt aaaataattt tttgattctt tgctaaaaac
     3901 aacccgatct caccttcttt ttaccggaat ttttcacaac ctctgcagca caaaaccatg
     3961 caaatgatgt atcaaaccat ctacaaggca cttgtggaag ttggtcttga tggccagctt
     4021 gagccacaag actttttgaa ctttttctgt cttggaacca gagaggttgg aaccagagag
     4081 gttcctgatg gaactgttag cgtatataat agtcctcgga agccacctca gctaaatgct
     4141 gcacaggtat acatatatta aatgtcttct tttgaaatct tcgattttgc cttaatgggt
     4201 tcgcttactg attaaaacga caaaccaatc taggtacaag ctttgaagag tcggagattc
     4261 atgatatatg ttcattccaa aggtatggta gtggatgatg agtttgtctt aattggttcc
     4321 gcgaatatca accagagatc cttggaagga actagagaca ctgaaatcgc tatgggaggg
     4381 tatcagccac accattcatg ggctaagaaa ggttctcgtc ctcgtggtca ggtaaataag
     4441 actttcagag acttctactt ctcaatctag gatagcattc taagacgaaa atctagaaac
     4501 ccaccaatgt atgttctctt taacatgtct gaaccctggt ttgtctcaat ttcttgcaga
     4561 tctttggata tagaatgtcg ttatgggcag aacatctagg gtttctagag caagaattcg
     4621 aggaaccaga gaacatggaa tgcgtgagac gagttaggca attgagtgag ctgaattgga
     4681 gacaatatgc agcggaagaa gtcacagaga tgccaggtca tcttcttaag tatccagttc
     4741 aagttgatag aacaggcaaa gtgagttctc ttcctggata cgagacattc ccagatctcg
     4801 gtggcaagat cataggatcg ttccttgtag tagaagaaaa cctcaccata aactcgagat
     4861 atctagaccc agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
     4921 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgcag
     4981 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     5041 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
     5101 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     5161 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
     5221 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     5281 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     5341 tttatccgta ctcctggtga tgcatggtta ctcaccactg cgatccccgg aaaaacagca
     5401 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     5461 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     5521 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     5581 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     5641 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     5701 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     5761 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     5821 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     5881 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacattattc agattgggcc
     5941 ccgttccact gagcgtcaga ccccgtagaa aagatca
//
