LOCUS       sequence-415377-       10892 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of WT SAMD9L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10892)
  AUTHORS   Russell AJ, Gray PE, Ziegler JB, Kim YJ, Smith S, Sewell WA, Goodnow
            CC
  TITLE     SAMD9L autoinflammatory or ataxia pancytopenia disease mutations 
            activate cell-autonomous translational repression.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Aug 24;118(34):e2110190118. doi: 
            10.1073/pnas.2110190118.
  PUBMED    34417303
REFERENCE   2  (bases 1 to 10892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-08-24"; volume: "118(34):e2110190118. 
            doi"; pages: " 10.1073/pnas.2110190118"
COMMENT     Sequence Label: pcDNA3.1+N-eGFP_SAMD9L
FEATURES             Location/Qualifiers
     source          1..10892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             923..1636
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             join(923..925,926..1636)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(965..986)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1226..1245)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1573..1594
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6483..6500)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6489..6713
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6759..7187
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6846..6865)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7056..7077
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7196..7216)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7201..7530
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7381..7516
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7443..7462
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7597..8391
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7651..7670)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8261..8280
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8565..8686
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8602..8621)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8656..8675
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8735..8751)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8735..8751)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8748..8770)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8759..8775
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8783..8789,8790..8807,8808..8813))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8828..8849
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8966..8983)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9137..9725)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9217..9236)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9896..10687,10688..10756))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10519..10538
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10757..10861)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      961 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     1021 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     1081 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     1141 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     1201 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     1261 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     1321 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     1381 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     1441 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     1501 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     1561 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctcacggcat
     1621 ggacgagctg tacaagggta ccgagctcgg atccgaattc tgcagatatc cagcacagtg
     1681 gcggccgctt atgagtaaac aagtatctct acctgaaatg attaaagact ggaccaaaga
     1741 gcatgtgaaa aaatgggtaa atgaagacct taagattaat gagcaatacg ggcaaattct
     1801 gctcagtgaa gaagtaacag gattagtcct gcaggaatta actgagaagg accttgtaga
     1861 aatggggcta ccatggggtc cagcactttt gataaaacgt tcatacaaca aattgaatag
     1921 taagtcccct gaaagtgaca atcatgatcc gggacaatta gataattcaa aaccgtccaa
     1981 aacagaacac cagaaaaatc caaaacacac caaaaaggaa gaagaaaatt caatgtcatc
     2041 taatattgat tatgatccca gagagatcag agatatcaaa caagaagaat caattcttat
     2101 gaaagaaaat gtgttagatg aagtagcaaa tgctaaacac aagaaaaagg gtaagctaaa
     2161 acctgaacaa ttgacttgta tgccatatcc ttttgatcag ttccatgaca gccatcgcta
     2221 catagaacat tatactctac aacctgaaac aggagcactc aatctcattg atccaataca
     2281 tgagttcaaa gctctcacaa acacagaaac agccacggaa gtggacatta agatgaaatt
     2341 cagcaatgaa gtcttccgat ttgcatcagc ttgtatgaat tcacgcacca atggcaccat
     2401 ccattttgga gtcaaggaca aaccccatgg agaaattgtt ggtgtgaaaa tcaccagtaa
     2461 ggctgccttc attgaccact tcaatgtaat gatcaaaaag tattttgaag aaagtgagat
     2521 caatgaagcc aagaagtgta ttcgggagcc aaggtttgtg gaagtccttc tgcagaacaa
     2581 tacaccatct gacagatttg tcattgaagt tgatactatt ccaaaacact ctatatgtaa
     2641 tgataagtat ttctacattc agatgcaaat ttgtaaagat aaaatatgga aacaaaacca
     2701 aaatctttca ctgtttgtaa gagaaggggc tagctctagg gatatcctgg ccaattccaa
     2761 gcaacgggat gtagatttca aggcattttt acaaaattta aagtcactgg tagcatctag
     2821 aaaagaggct gaagaagagt atggaatgaa ggcaatgaag aaggagagtg aaggactaaa
     2881 gctggttaaa cttctcatag gaaaccgaga ctcactggat aattcatact atgactggta
     2941 cattcttgta acaaataaat gccatccaaa ccaaataaag cacttagatt ttttaaaaga
     3001 aattaaatgg tttgctgtgt tggagtttga tcctgaatct atgatcaatg gagtggtcaa
     3061 agcttacaaa gaaagtcggg tggcaaacct tcactttcca aatcaatatg aagacaagac
     3121 aactaacatg tgggagaaga tttctactct taatctttac caacagccca gctggatttt
     3181 ctgcaacggc agatcagacc tgaaaagcga gacatataaa cctctagaac cacatttatg
     3241 gcagagagaa agagcttcag aagtcaggaa actaatttta tttctcacag atgaaaatat
     3301 aatgacaaga ggaaaatttt tggtagtgtt tctattactc tcttcagtgg aaagcccagg
     3361 agatccactc attgaaactt tctgggcttt ctatcaagct ctcaaaggaa tggaaaatat
     3421 gttgtgtatc tctgtaaact cacatattta tcaacgatgg aaagatctac tacaaacaag
     3481 aatgaagatg gaagatgaac taacaaacca cagtatttcc actttaaata tagaactggt
     3541 aaacagcact atccttaaac taaaatcggt gactcggtca tcaagaaggt ttttgcccgc
     3601 ccgtggatct tcttcagtta tcctagagaa aaagaaagag gatgtcttga ctgcactgga
     3661 aatcctctgt gaaaatgagt gtacagagac agacatcgag aaagacaaat ctaaattcct
     3721 ggagtttaag aaatcaaaag aagaacactt ttatcgaggt ggcaaagtat cctggtggaa
     3781 cttctatttt tcttctgaaa actattcttc agattttgtt aaaagggaca gttatgaaaa
     3841 gcttaaagat ttaatacact gctgggcaga gtctcctaaa ccaatatttg caaaaatcat
     3901 caatctttat catcatccag gctgtggagg taccacactg gctatgcatg ttctctggga
     3961 cttaaagaaa aacttcagat gtgctgtgtt aaaaaacaag acaactgatt ttgcagaaat
     4021 tgcagagcaa gtgatcaatc tggtcaccta tagggcaaag agccatcagg attacattcc
     4081 tgtgcttctc cttgtggatg attttgaaga acaagaaaat gtctactttc tacaaaatgc
     4141 catccattcc gttttagcag aaaaggattt gcgatatgaa aaaacattgg taattatctt
     4201 aaactgcatg agatcccgga atccagatga aagtgcaaaa ttggcagaca gtattgcact
     4261 aaattaccaa ctttcttcca aggaacaaag agcttttggt gccaaactga aggaaattga
     4321 aaagcagcac aagaactgtg aaaactttta ttccttcatg atcatgaaaa gcaattttga
     4381 tgaaacatat atagaaaatg tagtcaggaa tatcctaaaa ggacaggatg ttgacagcaa
     4441 ggaagcacaa ctcatttcct tcctggcttt actcagctct tatgttactg actctacaat
     4501 ttcagtttca cagtgtgaaa tatttttggg aatcatatac actagtacac cctgggaacc
     4561 tgaaagctta gaagacaaga tgggaactta ttctacactt ctaataaaaa cagaagttgc
     4621 agaatatggg agatacacag gtgtgcgtat cattcaccct ctgattgccc tgtactgtct
     4681 aaaagaactg gaaagaagct atcacttgga taaatgtcaa attgcattga atatattaga
     4741 agagaattta ttctatgatt ctggaatagg aagagacaaa tttcaacatg atgttcaaac
     4801 tcttctgctt acaagacagc gcaaggtgta tggagatgaa acagacactc tgttttcccc
     4861 attaatggaa gctttacaga ataaagacat tgaaaaggtc ttgagtgcag gaagtagacg
     4921 attcccacaa aatgcattca tttgtcaagc cttagcaaga catttctaca ttaaagagaa
     4981 ggactttaac acagctctgg actgggcacg tcaggccaaa atgaaagcac ctaaaaattc
     5041 ctatatttca gatacactag gtcaagtcta caaaagtgaa atcaaatggt ggttggatgg
     5101 gaacaaaaac tgtaggagca ttactgttaa tgacctaaca catctcctag aagctgcgga
     5161 aaaagcctca agagctttca aagaatccca aaggcaaact gatagtaaaa actatgaaac
     5221 cgagaactgg tcaccacaga agtcccagag acgatatgac atgtataaca cagcttgttt
     5281 cttgggtgaa atagaagttg gtctttacac tatccagatt cttcagctca ctcccttttt
     5341 ccacaaagaa aatgaattat ccaaaaaaca tatggtgcaa tttttatcag gaaagtggac
     5401 cattcctcct gatcccagaa atgaatgtta tttggctctt agcaagttca catcccacct
     5461 aaaaaattta caatcagatc tgaaaaggtg ctttgacttt tttattgatt atatggttct
     5521 tctgaaaatg aggtataccc aaaaagaaat tgcagaaatc atgttaagca agaaagtcag
     5581 tcgttgtttc aggaaataca cagaactttt ctgtcatttg gatccatgtc tattacaaag
     5641 taaagagagt caattactcc aggaggagaa ttgcaggaaa aagctagaag ctctgagagc
     5701 agataggttt gctggactct tggaatatct taatccaaac tacaaagatg ctaccaccat
     5761 ggaaagtata gtgaatgaat atgccttcct actgcagcaa aactcaaaaa agcccatgac
     5821 aaatgagaaa caaaattcca ttttggccaa cattattctg agttgtctaa agcccaactc
     5881 caagttaatt caaccactta ccacgctaaa aaaacaactc cgagaggtct tgcaatttgt
     5941 aggactaagt catcaatatc caggtcctta tttcttggcc tgcctcctgt tctggccaga
     6001 aaatcaagag ctagatcaag attccaaact aatagaaaag tatgtttcat ccttaaatag
     6061 atccttcagg ggacagtaca agcgcatgtg caggtccaag caggcaagca cacttttcta
     6121 tctgggcaaa aggaagggtc taaacagtat tgttcacaag gccaaaatag agcagtactt
     6181 tgataaagca caaaatacaa attccctctg gcacagtggg gatgtgtgga aaaaaaatga
     6241 agtcaaagac ctcctgcgtc gtctaactgg tcaggctgaa ggcaagctaa tctctgtaga
     6301 atatggaaca gaggaaaaaa taaaaatacc agtaatatct gtttattcag gtccactcag
     6361 aagtggtagg aacatagaaa gagtgtcttt ctacctagga ttttccattg aaggccctct
     6421 ggcatatgat atagaagtaa tttaactcga gtctagaggg cccgtttaaa cccgctgatc
     6481 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     6541 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     6601 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     6661 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     6721 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     6781 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     6841 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     6901 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     6961 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     7021 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     7081 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     7141 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     7201 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7261 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     7321 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     7381 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7441 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     7501 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     7561 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     7621 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     7681 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     7741 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     7801 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     7861 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     7921 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     7981 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     8041 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     8101 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     8161 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     8221 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     8281 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     8341 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     8401 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     8461 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     8521 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     8581 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     8641 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     8701 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     8761 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     8821 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     8881 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     8941 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     9001 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     9061 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     9121 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     9181 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     9241 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     9301 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     9361 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     9421 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     9481 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     9541 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     9601 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     9661 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     9721 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     9781 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     9841 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     9901 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     9961 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    10021 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    10081 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    10141 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    10201 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    10261 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    10321 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    10381 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    10441 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    10501 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    10561 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    10621 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    10681 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    10741 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    10801 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    10861 gcacatttcc ccgaaaagtg ccacctgacg tc
//
