LOCUS       sequence-415380-       12623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rat Cav2.1 calcium channel with R57A and R59A 
            mutations that prevent G-protein modulation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12623)
  AUTHORS   Dahimene S, Page KM, Nieto-Rostro M, Pratt WS, D'Arco M, Dolphin AC
  TITLE     A CaV2.1 N-terminal fragment relieves the dominant-negative 
            inhibition by an Episodic ataxia 2 mutant.
  JOURNAL   Neurobiol Dis. 2016 Sep;93:243-56. doi: 10.1016/j.nbd.2016.05.020. 
            Epub 2016 May 31.
  PUBMED    27260834
REFERENCE   2  (bases 1 to 12623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neurobiol 
            Dis."; date: "2016-09"; volume: "93:243-56. doi"; pages: " 
            10.1016/j.nbd.2016.05.020. Epub 2016 May 31"
COMMENT     Sequence Label: Cav2.1 R57A, R59A  pMT2
FEATURES             Location/Qualifiers
     source          1..12623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             84..647
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     polyA_signal    834..968
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(871..890)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     925..944
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1641..1658)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1812..2400)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1892..1911)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2571..3362,3363..3431))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3194..3213
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3432..3536)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3604..3622
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3660..3682)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3782..3801
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3995..4017
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4009..4026
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4010..4026
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4036..4365)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4050..4185
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4350..4370
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     intron          4834..5063
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5046..5065
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             11603..11632
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 gacagctcag ggctgcgatt tcgcgccaaa cttgacggca atcctagcgt gaaggctggt
       61 aggattttat ccccgctgcc atcatggttc gaccattgaa ctgcatcgtc gccgtgtccc
      121 aaaatatggg gattggcaag aacggagacc taccctggcc tccgctcagg aacgagttca
      181 agtacttcca aagaatgacc acaacctctt cagtggaagg taaacagaat ctggtgatta
      241 tgggtaggaa aacctggttc tccattcctg agaagaatcg acctttaaag gacagaatta
      301 atatagttct cagtagagaa ctcaaagaac caccacgagg agctcatttt cttgccaaaa
      361 gtttggatga tgccttaaga cttattgaac aaccggaatt ggcaagtaaa gtagacatgg
      421 tttggatagt cggaggcagt tctgtttacc aggaagccat gaatcaacca ggccacctca
      481 gactctttgt gacaaggatc atgcaggaat ttgaaagtga cacgtttttc ccagaaattg
      541 atttggggaa atataaactt ctcccagaat acccaggcgt cctctctgag gtccaggagg
      601 aaaaaggcat caagtataag tttgaagtct acgagaagaa agactaacag gaagatgctt
      661 tcaagttctc tgctcccctc ctaaagctat gcatttttat aagaccatgg gacttttgct
      721 ggctttagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
      781 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
      841 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
      901 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
      961 tctggatccc cggccaacgg tctggtgacc cggctgcgag agctcggtgt acctgagacg
     1021 cgagtaagcc cttgagtcaa agacgtagtc gttgcaagtc cgcaccaggt actgatatcc
     1081 caccaaaaag tgcggcggcg gctggcggta gaggggccag cgtagggtgg ccggggctcc
     1141 gggggcgagg tcttccaaca taaggcgatg atatccgtag atgtacctgg acatccaggt
     1201 gatgccggcg gcggtggtgg aggcgcgcgg aaagtcgcgg acgcggttcc agatgttgcg
     1261 cagcggcaaa aagtgctcca tggtcgggac gctctggccg gtgaggcgtg cgcagtcgtt
     1321 gacgctctag accgtgcaaa aggagagcct gtaagcgggc actcttccgt ggtctggtgg
     1381 ataaattcgc aagggtatca tggcggacga ccggggttcg aaccccggat ccggccgtcc
     1441 gccgtgatcc atgcggttac cgcccgcgtg tcgaacccag gtgtgcgacg tcagacaacg
     1501 ggggagcgct ccttttggct tccttccagg cgcggcggct gctgcgctag cttttttggc
     1561 gagctcgaat taattctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     1621 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1681 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     1741 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1801 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1861 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1921 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1981 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2041 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2101 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2161 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2221 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     2281 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2341 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2401 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2461 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2521 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     2581 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     2641 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     2701 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     2761 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     2821 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     2881 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     2941 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3001 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     3061 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     3121 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     3181 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     3241 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     3301 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3361 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     3421 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3481 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     3541 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     3601 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     3661 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     3721 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     3781 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     3841 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
     3901 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     3961 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     4021 gccagtgcca agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     4081 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     4141 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     4201 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     4261 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
     4321 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acaggatccg
     4381 gtcgcgcgaa tttcgagcgg tgttccgcgg tcctcctcgt atagaaactc ggaccactct
     4441 gagacgaagg ctcgcgtcca ggccagcacg aaggaggcta agtgggaggg gtagcggtcg
     4501 ttgtccacta gggggtccac tcgctccagg gtgtgaagac acatgtcgcc ctcttcggca
     4561 tcaaggaagg tgattggttt ataggtgtag gccacgtgac cgggtgttcc tgaagggggg
     4621 ctataaaagg gggtgggggc gcgttcgtcc tcactctctt ccgcatcgct gtctgcgagg
     4681 gccagctgtt gggctcgcgg ttgaggacaa actcttcgcg gtctttccag tactcttgga
     4741 tcggaaaccc gtcggcctcc gaacggtact ccgccaccga gggacctgag cgagtccgca
     4801 tcgaccggat cggaaaacct ctcgactgtt ggggtgagta ctccctctca aaagcgggca
     4861 tgacttctgc gctaagattg tcagtttcca aaaacgagga ggatttgata ttcacctggc
     4921 ccgcggtgat gcctttgagg gtggccgcgt ccatctggtc agaaaagaca atctttttgt
     4981 tgtcaagctt gaggtgtggc aggcttgaga tctggccata cacttgagtg acaatgacat
     5041 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcagg cgagcctgaa
     5101 ttcctcgagg tcttgcagaa tggcccgctt tggagacgag atgccgggcc gctacggcgc
     5161 aggcggagga ggctcagggc cggccgccgg ggtggtcgtg ggcgccgcgg gcggccgagg
     5221 agccgggggc agccggcagg gcgggcagcc cggagcgcag aggatgtaca agcagtcgat
     5281 ggcgcaggca gcggcgacca tggccctcta caaccccatc cctgtccgcc agaactgcct
     5341 cacggtcaac cgctccctgt tcctcttcag tgaagacaac gtggtgagaa aatacgccaa
     5401 aaagatcacg gaatggcctc ccttcgagta catgatcctg gccaccatca ttgctaactg
     5461 catcgtcctg gccctggagc agcacctccc tgatgatgac aagacaccca tgtccgagcg
     5521 gctggatgac acagaaccct atttcattgg catcttctgt tttgaggctg gaattaagat
     5581 cgtggctctt ggctttgcct tccacaaagg ctcctacctg aggaatggct ggaacgtcat
     5641 ggactttgtc gtggtgctaa caggcatctt ggccactgtc gggacggagt ttgatctacg
     5701 gacactgagg gcggttcgtg tgctgcggcc actcaagctg gtgtctggaa tcccaagttt
     5761 acaagtcgtc ctgaagtcaa tcatgaaggc gatgatccct ctgctgcaga tcggcctcct
     5821 cctgtttttt gcaatcctta tttttgcaat catagggtta gaattttata tgggaaaatt
     5881 tcataccacc tgctttgaag aggggacaga cgacatccag ggtgagtcgc cagctccgtg
     5941 tgggacagag gagcctgccc gcacctgccc caacgggacc aaatgtcagc cgtactggga
     6001 agggcccaac aacggcatca ctcagttcga caacatcctg tttgctgtgc tcactgtttt
     6061 ccagtgcatc accatggaag gctggactga tctcctctac aatagcaacg atgcctcagg
     6121 gaacacttgg aactggttgt acttcatccc cctcatcatc atcggctcct tttttatgct
     6181 gaaccttgtg ctgggtgtgc tgtctgggga gtttgccaaa gaaagggaac gtgtagagaa
     6241 ccgaagggct tttctgaagc tcagaagaca acagcagatt gaacgtgagc tcaatggata
     6301 catggagtgg atctcgaaag cagaagaggt gatcctcgcg gaggacgaaa cagacgtgga
     6361 gcagaggcac ccttttgatg gagctcttcg gagagctact ctgaagaaaa gcaagacgga
     6421 cctgctcaac cctgaggagg cggaggacca gcttgctgac atcgcgtctg tggggtctcc
     6481 cttcgccaga gccagcatca aaagtgccaa gctggagaat tcgacttttt tccacaaaaa
     6541 ggagagaaga atgcgtttct acatccgccg catggtcaaa actcaggcct tctactggac
     6601 cgtgctcagt ctggtagccc tcaacacgct gtgcgtcgcc attgtccact acaaccagcc
     6661 cgagtggctc tccgacttcc tctactatgc agaattcatt ttcttaggac tctttatgtc
     6721 cgaaatgttt ataaaaatgt atgggctcgg gacacggcct tacttccact cttccttcaa
     6781 ctgctttgac tgtggggtca tcatcgggag catctttgaa gtcatctggg ccgtcatcaa
     6841 accgggtaca tcctttggaa tcagcgtgtt acgagctctc aggttactgc gtattttcaa
     6901 agtcacaaag tactgggcat ctctcagaaa cctggttgtc tccctcctca actccatgaa
     6961 atccatcata agtctgctgt tcctcctctt cctcttcatt gtcgtctttg ccctcttggg
     7021 gatgcagctg tttggtggcc agtttaattt tgacgagggg actcctccca ccaacttcga
     7081 cacttttcca gcagcaataa tgactgtgtt tcagatcctg actggcgagg attggaatga
     7141 ggtcatgtat gatgggatca agtctcaggg gggcgtgcag ggcggcatgg tgttctccat
     7201 ctacttcatc gtcctcaccc tcttcgggaa ctacaccctg ctgaacgtgt tcttggctat
     7261 cgcggtggac aacctggcca acgcccagga actcaccaag gatgaacaag aagaggaaga
     7321 ggcagccaat cagaaactgg ctctacagaa agccaaggag gtggcagaag tgagtcccct
     7381 gtctgcagcc aacatgtcca tagctgtgaa ggaacagcag aagaaccaga agcctgccaa
     7441 gtcggtgtgg gagcagcgca ccagcgagat gcgcaagcag aacctgctgg ctagccgcga
     7501 ggcgctgtac ggggacgcgg ctgagcgctg gcccaccact tacgcgcgcc cgctgcggcc
     7561 ggacgtgaag acgcacttgg accggccgct cgtggtggac ccgcaggaga accgtaacaa
     7621 caacaccaac aagagccgtg cgccagaagc gctgcgccaa accgcgcggc cccgcgagag
     7681 cgcgcgcgac cccgacgcgc ggcgcgcctg gcccagcagc cctgagcgcg cccctggacg
     7741 agagggcccg tatggccgcg agagcgagcc gcaacagcgc gagcacgcgc caccccgcga
     7801 gcacgtaccc tgggacgcgg atcctgagcg cgccaaggcc ggggacgcgc cccgccgcca
     7861 cacgcaccgg cctgtggccg agggcgagcc tcgtcgccac cgcgcgcgcc gccggcccgg
     7921 ggacgaaccg gacgacagac cggagcgcag gccgcgtccc cgcgacgcca ctaggccggc
     7981 ccgcgctgca gacggcgaag gcgatgatgg ggagcgcaag cggcgacacc gacacgggcc
     8041 gccggcccac gatgacaggg agcgcagaca ccggcgcaga aaagagagcc agggctctgg
     8101 ggtccccatg tctggtccca acctgtccac caccaggcca atccagcagg atctgggccg
     8161 ccaggacctg ccactggctg aggacctgga caacatgaag aacaacaagt tggccaccgg
     8221 ggagcctgcc agtccccacg acagcctggg ccacagtggc cttcccccta gccctgccaa
     8281 gatcgggaac agcaccaacc ctggtcccgc cttggccacc aatccccaga atgctgccag
     8341 ccgcaggacg cccaacaacc cgggcaaccc gtccaacccc ggccccccca agactcccga
     8401 gaacagcctt atcgtcacca accccagcag cacccagccc aactcagcaa agactgccag
     8461 gaaacccgag cacatggcgg tggagatccc cccggcctgc ccgcccctca accacactgt
     8521 ggtccaagta aacaaaaacg ccaacccaga cccactgcca aagaaagagg aagagaagaa
     8581 ggaggaagag gaggcagacc ccggggagga tggcccaaag cccatgccgc cctacagctc
     8641 catgttcatc ctctccacca ccaaccccct tcgccggctg tgccattaca tcctgaacct
     8701 gcgctacttc gagatgtgca tcctcatggt cattgccatg agtagcatcg cgctggccgc
     8761 cgaggacccg gtgcagccca acgcaccccg caacaacgtg ctgcgatatt ttgactatgt
     8821 tttcacagga gtgtttacct ttgagatggt gatcaagatg atcgacctgg gcctcgtcct
     8881 gcatcagggg gcctatttcc gtgacctgtg gaacattctg gacttcatag tggtcagtgg
     8941 ggccctggtg gcctttgcct tcactggcaa tagcaaagga aaggacatca acaccatcaa
     9001 gtccctccga gtcctccggg tgctacgacc tctaaagacc atcaagcggc tgcctaagtt
     9061 gaaggccgta tttgactgcg tggtgaactc gctcaagaac gtcttcaaca tcctcattgt
     9121 ctacatgctc ttcatgttca tcttcgccgt ggtggccgtg cagctcttca agggcaaatt
     9181 cttccactgc acggacgagt ccaaggagtt tgagagagac tgtcgaggca aatacctcct
     9241 ttacgagaag aacgaggtaa aggcgcggga ccgcgagtgg aagaaatacg acttccacta
     9301 cgacaacgtg ctctgggccc tgctcacgct ctttacggtg tccacgggag agggctggcc
     9361 acaggtcctc aagcactcag tggatgccac ttttgagaac cagggcccca gccccgggta
     9421 ccgcatggaa atgtccatct tctacgtggt ctactttgtg gtgtttccct tcttctttgt
     9481 caatatcttt gtggccttga tcatcatcac cttccaggag cagggagaca agatgatgga
     9541 agaatacagc ctagagaaaa atgagagggc ctgcatcgac tttgccatca gtgccaagcc
     9601 gctgaccagg cacatgcccc agaacaagca gagcttccag tatcgaatgt ggcagttcgt
     9661 ggtgtcccca ccctttgagt acaccatcat ggccatgatc gctctcaaca ccatcgtgct
     9721 aatgatgaag ttctatggag cctctgtggc ctatgaaaac gcccttcgag tgttcaacat
     9781 tgtcttcacc tccctcttct ctctcgaatg tgtgctcaaa gtcatggctt ttgggattct
     9841 gaattatttc cgcgatgcct ggaacatctt cgactttgtg actgttctgg gcagcatcac
     9901 agacatcctc gtcaccgagt ttgggaataa cttcatcaac ctgagctttc tccgcctctt
     9961 ccgtgctgcc cgactcatca aactcctccg ccagggttac accatccgca ttctcctctg
    10021 gactttcgtg cagtctttca aggccctacc ttatgtctgt ctgctgatcg ccatgctctt
    10081 cttcatctat gccatcatcg ggatgcaggt gtttggcaac atcggcattg atggggaaga
    10141 tgaggacagc gatgaggatg agttccaaat cacgaggcac aataacttcc ggaccttctt
    10201 ccaagctctc atgcttctct tccggagcgc cacaggggaa gcgtggcaca acatcatgct
    10261 gtcctgcctc agcgggaagc catgcgacaa gaactccggg atccaaaaac cagagtgtgg
    10321 caacgagttc gcctattttt actttgtctc gttcatcttc ctttgctcat ttctgatgct
    10381 gaatctcttt gttgctgtca tcatggacaa cttcgagtac ctcacccgag attcctccat
    10441 cctgggcccc caccacctgg atgagtacgt gcgtgtctgg gcagagtatg accctgctgc
    10501 ctgcggccgc attcactata aggacatgta cagtttattg cgagtaatat cgccccctct
    10561 cggcttaggc aagaaatgtc ctcatagggt tgcttgcaag aggctcttgc ggatggacct
    10621 acccgtagcg gatgacaaca ccgttcactt caactccacc ttgatggctc tgatccgaac
    10681 cgccctggat atcaaaatcg caaagggtgg agctgacaag cagcaaatgg acgcagagct
    10741 ccgcaaggaa atgatggcca tttggcccaa cctgtctcag aagaccttgg atctgctggt
    10801 cacacctcac aagtccacgg acctgacagt gggtaagatc tacgcagcca tgatgatcat
    10861 ggagtactac cggcagagca aggccaagaa gctgcaggcc atgcgagagg agcagaaccg
    10921 gacaccactc atgttccagc gcatggagcc tccatcgcca acacaggagg gaggacccag
    10981 ccaaaacgcc cttccctcca ctcagctgga ccccggagga ggcctgatgg ctcaagaaag
    11041 cagcatgaag gagagcccgt cctgggtgac ccagcgggca caggagatgt tccagaagac
    11101 tggtacctgg agcccagagc gagggccacc catcgacatg cctaacagcc agcccaactc
    11161 ccagtctgtg gagatgagag aaatgggaac tgatggctac tcagacagcg aacactacct
    11221 ccccatggaa ggacagacca gggccgcctc catgccccgc ctcccagcag agaaccagag
    11281 gagaaggggc cggccacgtg gaaataacct cagtaccatc tctgatacca gccccatgaa
    11341 gcgctcagcc tccgtgctgg gacccaaagc ccggcgactg gatgactact cactagagcg
    11401 ggtaccacct gaggagaacc aaaggtacca ccaacgccgc cgggaccgtg gccaccgcac
    11461 ctctgagcgc tctctgggcc gatacactga tgtggacaca ggcctgggga cagatctgag
    11521 catgaccacc caatcgggtg acctgccctc caaagatcgg gaccaggacc ggggccggcc
    11581 caaggaccgg aagcatcggc cacaccacca ccaccaccat catcaccatc atcccccggc
    11641 cccggaccgg gagcgctacg cacaggagcg gccggacacc ggccgggcgc gggcccggga
    11701 gcagcgctgg tcccgctcgc ccagcgaggg tcgggagcac gcgacacaca gacagtagtt
    11761 ccgtcagtgg aagcccagcc ccctctacgt ccggcaccag cacgccgcgg cgcggccgcc
    11821 gccagctccc ccagaccccc tgcaccccgc ggccgcttgt gtcctactcg cccgctccgc
    11881 gcaggcctgc ggcgcgcagg atggcgggtc ccccagcgcc ccctggcggc tcgccgcggg
    11941 gttgccgccg tgcgccgcgc tggcctgcgc atgcgcctga gggcccgcgg ccgcgcggtg
    12001 ccgactacac cgagcccgac agcccgcgcg agccgccagg gggcgcgcat gagcccgcgc
    12061 cgcggtcgcc caggactccg cgcgccgcgg gctgcgcgtc accgcgccac ggccgcaggc
    12121 tgcccaatgg ttactacgcg gggcacggcg cgccacggcc gcgcacagcc cgcaggggcg
    12181 cgcacgacgc gtacagcgag agcgaggacg actggtgcta agccgccccg ccccccgcag
    12241 cgcccgtacg gtgcgggcgc accgcaaatg ataaacaata acgtttttaa aaaagaaaac
    12301 cggggagaaa aacaaaaata gcttctattg atgagtttta tcatctcgat tgaatctttc
    12361 gttttcccgg tgaacgaaac cgaggtggct gcgagccgga gggacggctg cacgccccac
    12421 acacttctca gacccacggg agttcttgcg ggccacgcca aacttattac tgcctgcaga
    12481 gttcaactac aagaaaataa cactaacagt ttttaaaagg actccgtaaa aagaaatttg
    12541 aaagagaatg tagagaggct gagaaagccc aggaattcct gcagcccggg ggatccacta
    12601 gtgcggccgc aattccgggg ggg
//
