LOCUS       sequence-415399-       10111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast 2µ vector for expression of Suc2SP-Notch1_100-sfGFP-GAL4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10111)
  AUTHORS   Ortner M, Guschtschin-Schmidt N, Stelzer W, Muhle-Goll C, Langosch D
  TITLE     Permissive Conformations of a Transmembrane Helix Allow 
            Intramembrane Proteolysis by gamma-Secretase.
  JOURNAL   J Mol Biol. 2023 Aug 1;435(18):168218. doi: 
            10.1016/j.jmb.2023.168218.
  PUBMED    37536392
REFERENCE   2  (bases 1 to 10111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2023-08-1"; volume: "435(18):168218. doi"; pages: " 
            10.1016/j.jmb.2023.168218"
COMMENT     Sequence Label: pYea5.1_Notch1_sfGFP
FEATURES             Location/Qualifiers
     source          1..10111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     11..30
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        188..531
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1628..4270
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             4277..4300
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     terminator      4304..4351
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4378..4565
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4433..4457)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(4589..4605)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4589..4605)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4602..4624)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4613..4629
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4637..4643,4644..4661,4662..4667))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4682..4703
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4820..4837)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4991..5579)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5071..5090)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5908..5955
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5980..5999
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(6187..6981)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(6187..6981)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6298..6317)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6908..6927
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6982..7086)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7113..8455
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(8076..8123)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     8496..8514
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8552..8574)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8674..8693
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8714..8995
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             8996..9670
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(join(9773..10111,1..117))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9910..9931)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
       61 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca
      121 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      181 acgccaggac atggaggccc agaataccct ccttgacagt cttgacgtgc gcagctcagg
      241 ggcatgatgt gactgtcgcc cgtacattta gcccatacat ccccatgtat aatcatttgc
      301 atccatacat tttgatggcc gcacggcgcg aagcaaaaat tacggctcct cgctgcagac
      361 ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct
      421 gtagagaaat ataaaaggtt aggatttgcc actgaggttc ttctttcata tacttccttt
      481 taaaatcttg ctaggataca gttctcacat cacatccgaa cataaacaac caagcttgaa
      541 gctagcctcc tgaaagatgc ttttgcaagc attccttttc cttttggctg gttttgcagc
      601 caagatctct gccgcattgg ctagtcttgg ttcacttaat atcccgtaca agattgaagc
      661 tgtgcaaagc gaaactgttg aacccccacc gcctgctcaa ttacacttca tgtacgtcgc
      721 tgccgctgcc tttgttcttt tatttttcgt tggttgcgga gttttgcttt caagaaaacg
      781 tcgtaggcaa catggtcagt tgtggtttcc tgaaggcttc aaagtttctg aagcttccaa
      841 aaaaaagcgt agagaaccgc ttggtgaaga cagcgttggc cttaaaccac ttaaaaatgc
      901 ttccgatggg gccggtacca tgtcaaaagg tgaagaatta tttacaggtg tagtccctat
      961 tctagtggag ttagatggag atgtcaatgg acataagttt tccgtcaggg gagagggaga
     1021 gggcgatgcc acaaatggaa agttaacatt gaagtttatt tgtacaacgg ggaagttacc
     1081 tgtgccttgg cctacattgg taacaacttt gacgtatgga gtccaatgtt tctcaaggta
     1141 tccagatcat atgaagcaac atgatttctt taagtcggcg atgcctgagg gctatgtaca
     1201 agagagaact atttccttta aggatgatgg tacttataag acgagagcag aggtgaagtt
     1261 tgagggtgat acgcttgtca ataggattga gttgaagggg atagatttta aggaagatgg
     1321 caatatcctg ggccataagt tggagtacaa ctttaattcc cataatgtct atattactgc
     1381 ggataagcag aagaatggaa ttaaggcaaa tttcaagatt aggcataatg tagaggatgg
     1441 gagtgtacaa ctagcagatc attatcaaca aaatacacct ataggcgatg gaccagtctt
     1501 actaccagat aatcattatt tatccacaca aagtgtctta tccaaggacc ctaatgagaa
     1561 gagagatcat atggtcctat tagagttcgt gacggcagca ggaattacgc atggtatgga
     1621 tgaactaatg aagctactga gctctatcga acaagcatgc gatatttgcc gacttaaaaa
     1681 gctcaagtgc tccaaagaaa aaccgaagtg cgccaagtgt ctgaagaaca actgggagtg
     1741 tcgctactct cccaaaacca aaaggtctcc gctgactagg gcacatctga cagaagtgga
     1801 atcaaggcta gaaagactgg aacagctatt tctactgatt tttcctcgag aagaccttga
     1861 catgattttg aaaatggatt ctttacagga tataaaagca ttgttaacag gattatttgt
     1921 acaagataat gtgaataaag atgccgtcac agatagattg gcttcagtgg agactgatat
     1981 gcctctaaca ttgagacagc atagaataag tgcgacatca tcatcggaag agagtagtaa
     2041 caaaggtcaa agacagttga ctgtatcgat tgactcggca gctcatcatg ataactccac
     2101 aattccgttg gattttatgc ccagggatgc tcttcatgga tttgattggt ctgaagagga
     2161 tgacatgtcg gatggcttgc ccttcctgaa aacggacccc aacaataatg ggttctttgg
     2221 cgacggttct ctcttatgta ttcttcgatc tattggcttt aaaccggaaa attacacgaa
     2281 ctctaacgtt aacaggctcc cgaccatgat tacggataga tacacgttgg cttctagatc
     2341 cacaacatcc cgtttacttc aaagttatct caataatttt cacccctact gccctatcgt
     2401 gcactcaccg acgctaatga tgttgtataa taaccagatt gaaatcgcgt cgaaggatca
     2461 atggcaaatc ctttttaact gcatattagc cattggagcc tggtgtatag agggggaatc
     2521 tactgatata gatgtttttt actatcaaaa tgctaaatct catttgacga gcaaggtctt
     2581 cgagtcaggt tccataattt tggtgacagc cctacatctt ctgtcgcgat atacacagtg
     2641 gaggcagaaa acaaatacta gctataattt tcacagcttt tccataagaa tggccatatc
     2701 attgggcttg aatagggacc tcccctcgtc cttcagtgat agcagcattc tggaacaaag
     2761 acgccgaatt tggtggtctg tctactcttg ggagatccaa ttgtccctgc tttatggtcg
     2821 atccatccag ctttctcaga atacaatctc cttcccttct tctgtcgacg atgtgcagcg
     2881 taccacaaca ggtcccacca tatatcatgg catcattgaa acagcaaggc tcttacaagt
     2941 tttcacaaaa atctatgaac tagacaaaac agtaactgca gaaaaaagtc ctatatgtgc
     3001 aaaaaaatgc ttgatgattt gtaatgagat tgaggaggtt tcgagacagg caccaaagtt
     3061 tttacaaatg gatatttcca ccaccgctct aaccaatttg ttgaaggaac acccttggct
     3121 atcctttaca agattcgaac tgaagtggaa acagttgtct cttatcattt atgtattaag
     3181 agattttttc actaatttta cccagaaaaa gtcacaacta gaacaggatc aaaatgatca
     3241 tcaaagttat gaagttaaac gatgctccat catgttaagc gatgcagcac aaagaactgt
     3301 tatgtctgta agtagctata tggacaatca taatgtcacc ccatattttg cctggaattg
     3361 ttcttattac ttgttcaatg cagtcctagt acccataaag actctactct caaactcaaa
     3421 atcgaatgct gagaataacg agaccgcaca attattacaa caaattaaca ctgttctgat
     3481 gctattaaaa aaactggcca cttttaaaat ccagacttgt gaaaaataca ttcaagtact
     3541 ggaagaggta tgtgcgccgt ttctgttatc acagtgtgca atcccattac cgcatatcag
     3601 ttataacaat agtaatggta gcgccattaa aaatattgtc ggttctgcaa ctatcgccca
     3661 ataccctact cttccggagg aaaatgtcaa caatatcagt gttaaatatg tttctcctgg
     3721 ctcagtaggg ccttcacctg tgccattgaa atcaggagca agtttcagtg atctagtcaa
     3781 gctgttatct aaccgtccac cctctcgtaa ctctccagtg acaataccaa gaagcacacc
     3841 ttcgcatcgc tcagtcacgc cttttctagg gcaacagcaa cagctgcaat cattagtgcc
     3901 actgaccccg tctgctttgt ttggtggcgc caattttaat caaagtggga atattgctga
     3961 tagctcattg tccttcactt tcactaacag tagcaacggt ccgaacctca taacaactca
     4021 aacaaattct caagcgcttt cacaaccaat tgcctcctct aacgttcatg ataacttcat
     4081 gaataatgaa atcacggcta gtaaaattga tgatggtaat aattcaaaac cactgtcacc
     4141 tggttggacg gaccaaactg cgtataacgc gtttggaatc actacaggga tgtttaatac
     4201 cactacaatg gatgatgtat ataactatct attcgatgat gaagataccc caccaaaccc
     4261 aaaaaaagag tccgcatgga gtcatccgca attcgaaaaa taactagcat aaccccttgg
     4321 ggcctctaaa cgggtcttga ggggtttttt gcgcgcttgc agccaagcta attccgggcg
     4381 aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac
     4441 aaattttaaa gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc
     4501 ctgtaggtca ggttgctttc tcaggtatag catgaggtcg ctcttattga ccacacctct
     4561 accggcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     4621 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     4681 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     4741 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     4801 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     4861 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     4921 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     4981 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     5041 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     5101 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     5161 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     5221 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     5281 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     5341 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     5401 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     5461 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     5521 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     5581 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     5641 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     5701 gaagttttaa atcaatctaa agtatatatg agtaacctga ggctatggca gggcctgccg
     5761 ccccgacgtt ggctgcgagc cctgggcctt cacccgaact tggggggtgg ggtggggaaa
     5821 aggaagaaac gcgggcgtat tggccccaat ggggtctcgg tggggtatcg acagagtgcc
     5881 agccctggga ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc gttttattct
     5941 gtctttttat tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag
     6001 ttagcctccc cctagggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca
     6061 tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg
     6121 aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag
     6181 tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc
     6241 ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat
     6301 atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc
     6361 gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg
     6421 agtcacgacg agatcctcgc cgtcgggcat gctcgccttg agcctggcga acagttcggc
     6481 tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat
     6541 ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg
     6601 atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc
     6661 aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc
     6721 cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga
     6781 tagccgcgct gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa
     6841 aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt
     6901 ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg
     6961 caatccatct tgttcaatca tactcttcct ttttcaatat tattgaagca tttatcaggg
     7021 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     7081 tccgcgcaca tttccccgaa aagtgccacc tgaacgaagc atctgtgctt cattttgtag
     7141 aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta
     7201 cagaacagaa atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt
     7261 tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat
     7321 ttttacagaa cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc
     7381 ttttttgttc tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat
     7441 tacttttttt ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta
     7501 ggtccgttaa ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc
     7561 ctgactccac ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga
     7621 taaaggcatc cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa
     7681 agtgatagcg ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg
     7741 tctctatata ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat
     7801 gaatagttct tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa
     7861 tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata
     7921 gggatatagc acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg
     7981 tattcgcaat attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc
     8041 gtcttcagag cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagagaat
     8101 aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa
     8161 cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt
     8221 atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg cgtacttata
     8281 tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt atcccattcc
     8341 atgcggggta tcgtatgctt ccttcagcac taccctttag ctgttctata tgctgccact
     8401 cctcaattgg attagtctca tccttcaatg ctatcatttc ctttgatatt ggatcatact
     8461 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     8521 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     8581 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     8641 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     8701 tgcaccatag atcaacgaca ttactatata tataatatag gaagcattta atagaacagc
     8761 atcgtaatat atgtgtactt tgcagttatg acgccagatg gcagtagtgg aagatattct
     8821 ttattgaaaa atagcttgtc accttacgta caatcttgat ccggagcttt tctttttttg
     8881 ccgattaaga attaattcgg tcgaaaaaag aaaaggagag ggccaagagg gagggcattg
     8941 gtgactattg agcacgtgag tatacgtgat taagcacaca aaggcagctt ggagtatgtc
     9001 tgttattaat ttcacaggta gttctggtcc attggtgaaa gtttgcggct tgcagagcac
     9061 agaggccgca gaatgtgctc tagattccga tgctgacttg ctgggtatta tatgtgtgcc
     9121 caatagaaag agaacaattg acccggttat tgcaaggaaa atttcaagtc ttgtaaaagc
     9181 atataaaaat agttcaggca ctccgaaata cttggttggc gtgtttcgta atcaacctaa
     9241 ggaggatgtt ttggctctgg tcaatgatta cggcattgat atcgtccaac tgcatggaga
     9301 tgagtcgtgg caagaatacc aagagttcct cggtttgcca gttattaaaa gactcgtatt
     9361 tccaaaagac tgcaacatac tactcagtgc agcttcacag aaacctcatt cgtttattcc
     9421 cttgtttgat tcagaagcag gtgggacagg tgaacttttg gattggaact cgatttctga
     9481 ctgggttgga aggcaagaga gccccgaaag cttacatttt atgttagctg gtggactgac
     9541 gccagaaaat gttggtgatg cgcttagatt aaatggcgtt attggtgttg atgtaagcgg
     9601 aggtgtggag acaaatggtg taaaagactc taacaaaata gcaaatttcg tcaaaaatgc
     9661 taagaaatag gttattactg agtagtattt atttaagtat tgtttgtgca cttgccgatc
     9721 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta
     9781 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     9841 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     9901 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     9961 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
    10021 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
    10081 agagcttgac ggggaaagcc ggcgaacgtg g
//
