LOCUS       sequence-415406-        5930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dimerization dependent fluorescent protein GA anchored to cytosolic 
            face of the caveolae with mouse CAVIN1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Miner GE, Smith SY, Showalter WK, So CM, Ragusa JV, Powers AE, 
            Zanellati MC, Hsu CH, Marchan MF, Cohen S
  TITLE     Contact-FP: A Dimerization-Dependent Fluorescent Protein Toolkit for
            Visualizing Membrane Contact Site Dynamics.
  JOURNAL   Contact (Thousand Oaks). 2024 Feb 4;7:25152564241228911. doi: 
            10.1177/25152564241228911. eCollection 2024 Jan-Dec.
  PUBMED    38327561
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Contact 
            (Thousand Oaks)."; date: "2024-02-4"; volume: "7:25152564241228911. 
            doi"; pages: " 10.1177/25152564241228911. eCollection 2024 Jan-Dec"
COMMENT     Sequence Label: Cav-GA
FEATURES             Location/Qualifiers
     source          1..5930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        16..319
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        320..523
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     474..494
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      575..584
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1923..1941)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2191..2210
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2385..2408
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    2673..2794
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2710..2729)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2764..2783
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2801..3256)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2938..2959)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3150..3169
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3283..3387
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3389..3746
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3389..3580
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3412..3432)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3597..3732
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3781..4575
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3781..4575
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3835..3854)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4445..4464
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4763..4782)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4807..4854
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5183..5771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5672..5691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
       61 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      121 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      181 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      241 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      301 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      361 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      421 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      481 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
      541 gatccgctag cgtgccgcca cccgctagcg tgccgccacc atggaggatg tcacgctcca
      601 tatcgttgag cggccgtatt ccggatttcc cgatgcttcc tcagagggcc cggagcccac
      661 ccaaggggag gcgcgggcca cggaggagcc gtcggggacc ggctccgacg agctgatcaa
      721 gtcggaccag gtgaacggtg tgctggtgct gagccttctg gataaaatca tcggcgccgt
      781 tgaccagatc cagctgaccc aagcccagct ggaggagcga caggcggaga tggagggcgc
      841 tgtgcagagc atccagggag agctgagcaa gctgggcaag gcgcacgcca ccacgagcaa
      901 caccgtgagc aagttgctgg agaaggtgcg caaggtcagc gtcaacgtga agaccgtgcg
      961 cggcagcctg gagcgccagg ccggccagat aaagaaactg gaggtcaacg aggcggagct
     1021 gctgaggcgc cgcaacttca aagtcatgat ctaccaggat gaagtcaagc tgccggccaa
     1081 actgagcgtc agcaagtcgc tgaaagagtc ggaggcactg cctgagaagg agggtgacga
     1141 gctgggcgag ggcgagcggc ccgaggatga caccgcggcg atcgagctgt cgtccgacga
     1201 ggcggtggag gtggaggagg tgatcgagga gtcccgcgcc gagcgcatca agcgcagcgg
     1261 cctgcggcgc gtggacgact tcaagaaggc cttctccaag gagaagatgg agaagaccaa
     1321 ggtgcgcacg cgtgagaacc tggagaagac gcgcctgaag accaaggaga acctggagaa
     1381 gacacggcac acgctggaga agcgcatgaa caagctgggc acgcgcctgg tgcccgtgga
     1441 gcgacgagag aagctgaaga catcccggga caagctgcgc aagtccttca cgcccgacca
     1501 tgtggtgtat gcgcgctcca agaccgctgt ctacaaggtg ccgcctttca ccttccacgt
     1561 caagaagatc cgcgagggcg aggtggaggt gctgaaggcc accgagatgg tggaggtggg
     1621 tcccgaggac gacgaggttg gcgcggagcg cggcgaggcc actgacctgc tgcgcgggag
     1681 cagccccgac gtgcacacgc tgctggagat caccgaggag tcggacgccg tcctggtgga
     1741 caagagcgac agcgacggtg gtggtggtag tggtggtggt ggtagtgcta tggtgagcaa
     1801 gagcgaggag gtcatcaaag agttcatgcg cttcaaggtg cgcttggagg gctccatgaa
     1861 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccttacgagg gcacccagac
     1921 cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
     1981 cctgatcatg tacggctcca agatgtacgt gaagcacccc gccgacgtcc ccgattacat
     2041 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgcacttcg aggacggcgg
     2101 tctggtgacc gcgacacagg actcctccct gcaggacggc acgctgatct acaaggtgaa
     2161 gatgcgcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccttgggctg
     2221 ggattatgcc accgagcgcc tgtaccccga agaaggcgtg ctgaagggcg agctcctggg
     2281 gcgcctgaag ctgaaggacg gcggcctcaa cctggtggag tccaagacca tctacatggc
     2341 caagaagccc gtgcaactgc ccggctacta cttcgtggac accaagctgg acatcacctc
     2401 ccacaacgag gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct
     2461 ggggcatggc accggcagca cctaatagaa gcttggtaaa ccgctgatca gcctcgactg
     2521 cagtcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc ataatcagcc
     2581 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     2641 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     2701 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2761 gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag cgttaatatt
     2821 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     2881 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     2941 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     3001 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     3061 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     3121 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     3181 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     3241 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     3301 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     3361 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt
     3421 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3481 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     3541 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     3601 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     3661 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     3721 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc
     3781 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     3841 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     3901 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     3961 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     4021 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     4081 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     4141 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     4201 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     4261 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
     4321 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     4381 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     4441 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     4501 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     4561 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     4621 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     4681 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     4741 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
     4801 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac
     4861 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
     4921 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
     4981 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac
     5041 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5101 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5161 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5221 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5281 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     5341 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5401 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5461 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5521 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5581 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5641 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5701 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5761 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5821 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca
     5881 tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc
//
