LOCUS       sequence-41541-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  TITLE     Glazebrook/ Katagiri Lab Gateway destination vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBPX_C1
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(86..92,93..110,111..116))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(141..265)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cggtggtata tccaggtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
       61 gacggatcct aactcaaaat ccacacatta tacgagccgg aagcataaag tgtaaagcct
      121 ggggtgccta atgcggccgc catagtgact ggatatgttg tgttttacag tattatgtag
      181 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
      241 gttcagcttt tttgtacaaa cttgtttgat agcttggcgc gcctcgaaat tccacggagc
      301 aaggggagag ggagccgaga gctgcttact acgtgaccgc cccgcgcctg ctgctgtccg
      361 gagctccaac ggagctggag agggggtgtg tgtggcgaga gagtacagcg aaaaggtgag
      421 aggcgaacgg gtgaatcctg cacgcggaat gcctttattc ttataccatc cgtggggcgc
      481 gacccaagct tgggcccaag cttggggcga aggatagtgg gattgtgcgt catcccttac
      541 gtcagtggag atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt
      601 ttccacgatg ctccttgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc
      661 ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc
      721 tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga
      781 tattaccc
//
