LOCUS       sequence-41542-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  TITLE     Glazebrook/ Katagiri Lab Gateway destination vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUNos_C1
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(88..94,95..112,113..118))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(143..267)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
       61 tcgacggatc ctaactcaaa atccacacat tatacgagcc ggaagcataa agtgtaaagc
      121 ctggggtgcc taatgcggcc gccatagtga ctggatatgt tgtgttttac agtattatgt
      181 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
      241 tcgttcagct tttttgtaca aacttgtttg atagcttggc gcgcctcgag gggatcctct
      301 agagtcgacc tgcagaagta acaccaaaca acagggtgag catcgacaaa agaaacagta
      361 ccaagcaaat aaatagcgta tgaaggcagg gctaaaaaaa tccacatata gctgctgcat
      421 atgccatcat ccaagtatat caagatcaaa ataattataa aacatacttg tttattataa
      481 tagataggta ctcaaggtta gagcatatga atagatgctg catatgccat catgtatatg
      541 catcagtaaa acccacatca acatgtatac ctatcctaga tcgatatttc catccatctt
      601 aaactcgtaa ctatgaagat gtatgacaca cacatacagt tccaaaatta ataaatacac
      661 caggtagttt gaaacagtat tctactccga tctagaacga atgaacgacc gcccaaccac
      721 accacatcat cacaaccaag cgaacaaaaa gcatctctgt atatgcatca gtaaaacccg
      781 catcaacatg tatacctatc ctagatcgat a
//
