LOCUS       sequence-415438-       12345 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rabbit Cav2.2 calcium channel with a N-terminal mEos 
            tag and a mutation (W391A) in the ?1-interacting domain (AID) in the
            I-II loop of Cav2.2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12345)
  TITLE     Dolphin Lab Plasmids 2023
REFERENCE   2  (bases 1 to 12345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos Cav2.2 W391A pRK5
FEATURES             Location/Qualifiers
     source          1..12345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      317..326
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    330..464
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(367..386)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     421..440
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        533..890
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        533..724
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(556..576)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      741..876
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(910..927)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(910..926)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(919..941)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1139..1594
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1226..1245)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1436..1457
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1611..1630)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1730..1752
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1790..1808)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1876..1980
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1981..2049,2050..2841)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2199..2218)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3012..3600
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3501..3520
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3754..3771
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3888..3909
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3924..3929,3930..3947,3948..3954)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3962..3978
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3967..3989
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3986..4002
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3986..4002
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        4031..4410
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4411..4614
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4565..4585
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4611..4635
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        4845..4863
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4845..4862
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     4876..4895
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             4948..5625
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gacccgctca gccggcccct ggcgcccggc tcgcgcatcg gctccgaccc ctacctgggg
       61 cagcggctgg acagcgaggc gcccgcccgt gccctgcccg aggacgcgcc cgcctttgag
      121 gagaccgcgg ccagcaactc gggccgctcc tcccggacct cgtacgtgtc ctcactgacc
      181 tcgcagcccc cgccgctgcg ccgcgtgccc aacggctacc actgcactct ggggctgggc
      241 gggggcggcc gggcgcggcg cggctgccac caccccgacc gcgaccgccg gtgctagact
      301 agttctagca agcttggccg ccatggccca acttgtttat tgcagcttat aatggttaca
      361 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
      421 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcgggaat taattcggcg
      481 cagcaccatg gcctgaaata acctctgaaa gaggaacttg gttaggtacc ttctgaggcg
      541 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
      601 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
      661 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
      721 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
      781 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
      841 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctgttaa
      901 cagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
      961 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     1021 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     1081 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     1141 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     1201 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     1261 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     1321 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     1381 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     1441 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
     1501 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     1561 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     1621 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     1681 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     1741 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     1801 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     1861 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     1921 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     1981 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     2041 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     2101 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     2161 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     2221 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     2281 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     2341 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     2401 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     2461 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     2521 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     2581 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     2641 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     2701 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     2761 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     2821 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     2881 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     2941 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3001 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3061 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3121 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3181 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3241 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3301 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3361 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3421 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3481 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3541 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3601 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3661 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3721 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3781 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     3841 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     3901 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     3961 attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac gaattaattc
     4021 gagctcgccc gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     4081 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     4141 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     4201 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     4261 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     4321 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     4381 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     4441 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     4501 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     4561 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
     4621 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
     4681 cgggaccgat ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc
     4741 aagagtgacg taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa
     4801 cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat
     4861 agaataacat ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc
     4921 tcggttctat cgattgaatt cggatccatg agtgcgatta agccagacat gaagatcaaa
     4981 ctccgtatgg aaggcaacgt aaacgggcac cactttgtga tcgacggaga tggtacaggc
     5041 aagccttttg agggaaaaca gagtatggat cttgaagtca aagagggcgg acctctgcct
     5101 tttgcctttg atatcctgac cactgcattc cattacggca acagggtatt cgccaaatat
     5161 ccagacaaca tacaagacta ttttaagcag tcgtttccta aggggtattc gtgggaacga
     5221 agcttgactt tcgaagacgg gggcatttgc attgccagaa acgacataac aatggaaggg
     5281 gacactttct ataataaagt tcgattttat ggtaccaact ttcccgccaa tggtccagtt
     5341 atgcagaaga agacgctgaa atgggagccc tccactgaga aaatgtatgt gcgtgatgga
     5401 gtgctgacgg gtgatattca tatggctttg ttgcttgaag gaaatgccca ttaccgatgt
     5461 gacttcagaa ctacttacaa agctaaggag aagggtgtca agttaccagg ctaccacttt
     5521 gtggaccact gcattgagat tttaagccat gacaaagatt acaacaaggt taagctgtat
     5581 gagcatgctg ttgctcattc tggattgcct gacaatgcca gacgagtccg cttcggggac
     5641 gagctgggcg gccgctatgg gggcgcgggc ggcgcggagc gggcccgggg cggcggggcc
     5701 ggcggggctg gcggcccggg ccccgggggg ctgccgccgg gccagcgggt cctctacaaa
     5761 cagtcgatcg cgcagcgcgc gcggaccatg gcgctgtaca accccatccc ggtcaagcag
     5821 aactgcttca ccgtcaaccg ctcgctcttc gtcttcagcg aggacaacgt cgtccgcaaa
     5881 tacgccaagc gcatcaccga gtggcctccg ttcgagtaca tgatcctggc caccatcatc
     5941 gccaactgca tcgtgctggc cctggagcag cacctccctg acggggacaa gacgcctatg
     6001 tccgagcgcc tggatgacac cgagccctac ttcattggga tcttctgctt cgaggcgggg
     6061 atcaagatcc tggccctggg ctttgttctg cacaagggct cctatctgcg gaacggctgg
     6121 aacgtcatgg acttcgtggt agtcctcacg gggattcttg ccacagccgg aaccgacttc
     6181 gacctgcgaa cgctgagggc tgtgcgtgtg ctaaggcccc tgaagctggt gtctgggatt
     6241 ccgagcttgc aggtggtgct caagtccatc atgaaggcca tggtcccgct gctgcagatc
     6301 gggctgctgc tcttcttcgc catcctcatg tttgccatca tcggcctcga gttctacatg
     6361 ggcaagttcc acaaggcctg cttccccaac agcacagacc cagatcccgt gggtgacttc
     6421 ccctgtggca aggaggcccc ggcccggctc tgtgagggag acacggagtg ccgggagtac
     6481 tgggccggac ccaactttgg tatcaccaac tttgacaaca tcctgtttgc catcttgacc
     6541 gtgttccagt gcatcaccat ggagggctgg actgacatcc tctacaatac aaacgatgca
     6601 gccggcaaca cgtggaactg gctctacttc atccctctca tcatcatcgg ctccttcttc
     6661 atgctcaacc tggtgctggg tgtgctctca ggagagtttg ctaaggagcg cgagagagtg
     6721 gagaaccgcc gcgccttcct gaagctccgg cggcagcagc agattgagcg ggagctcaac
     6781 gggtacctgg aggcgatctt caaggctgag gaagtcatgc tggctgagga ggacaggaac
     6841 gcagaggaga agtcccctct ggatgcagtg ttgaagagag cagctgccaa gaagagcaga
     6901 agcgacctga tccaggccga ggagggggag ggccggctca caggcctctg tgctcccggt
     6961 tccccgttcg cccgtgccag cctgaaaagc gggaagacgg agagctcgtc gtacttccgg
     7021 aggaaggaga agatgttccg cttcttcatc cggcgcatgg tgaaggcaca gagcttctac
     7081 tggacggtgc tgtgtgtggt ggcactgaac acactgtgcg tggccatggt gcactacaac
     7141 cagccgcagc ggctcaccac ggcattgtac tttgcagagt ttgttttcct gggtctcttc
     7201 ctcacagaga tgtccctgaa gatgtatggc ctggggccca ggagctactt ccggtcctcc
     7261 ttcaactgtt ttgactttgg ggtcatcgtg ggcagtatct ttgaagtggt ctgggctgct
     7321 gtcaagccag ggacctcctt cgggatcagt gttctgcggg ctctgcgctt actgagaatc
     7381 ttcaaggtca ccaaatactg gaactcccta cggaacctgg tggtgtctct gctcaactcc
     7441 atgaagtcca tcatcagcct cctcttcctg ctcttcctgt tcattgttgt ctttgctctg
     7501 ctggggatgc agctgtttgg aggccagttc aactttaaag acgagacccc aacgaccaac
     7561 tttgacacct tccctgctgc tatcctcacc gtcttccaga tcctgaccgg agaggactgg
     7621 aatgcggtca tgtaccacgg gatcgagtcg caaggtggcg tcagcagagg aatgttctcc
     7681 tccttctact tcattgtcct cactctgttt gggaactaca ccctgctgaa tgtcttcctg
     7741 gccattgctg tggacaacct tgccaatgct caggagctga ccaaggatga agaggagatg
     7801 gaagaagcag ccaatcagaa actcgccttg cagaaggcca aggaagtggc tgaagtcagc
     7861 cccatgtctg ctgcgaacat ctccattgcc gccaggcagc agaactcggc caaagcgcgc
     7921 tcggtgtggg agcagcgagc cagccagctg cggctgcaga acctgagggc cagctgcgag
     7981 gcgctgtaca gcgagatgga ccccgaggag aggctgcgct acgccaccgc gcgccacctg
     8041 cggcccgacg tgaagacgca cctggaccgg ccgctggtgg ttgagcccgg ccgcgacgct
     8101 ccgcggggcc ccccgggggg gaagtcccgg ccggatggct cggaggcccc cgagggtgcg
     8161 gatcccccgc gcaggcatca ccggcaccga gacaaggaca aggctcccgc cacggtcccc
     8221 tcggcggggg aacaggacag agccgaagct ctgagggcag agggcgggga gctggggccc
     8281 cgcgaggagc gggggcggcc acgccgcagc cgcagcaagg aggccccggg cgccccggag
     8341 gtgcgcagcg atcgcggccg tggcccgtgt cctgaaggtg gtcggcgcca ccaccggcgc
     8401 gggtcaccgg aggaggcagc tgagagggag ccccgccgcc accgcgcgca ccggcacggg
     8461 cccgacccgg gcaaggaggg cccagcgtcc gggaccaggg gcgagcggcg cgcacgacac
     8521 cgcacgggcc cccgggcgtg cccccgggag gctgagagca gcgaggagcc tgcgcggcgg
     8581 caccgggctc ggcacaaggc gccgcccacc caggagaccg cggagaagga caaggaggct
     8641 gccgagaagg ggggcgaggc cacggaggcc gagaaggaca aggaggctcg gaaccaccag
     8701 cccaaggagc tgccctgtga cctggaggcc atcggcatgc tgggagtggg cgctgtgcat
     8761 acgctgccca gcacctgtct gcagaaggtg gaggagcagc cagaggatgc ggacaaccag
     8821 cggaacgtca cccgcatggg tagtcagccc ccggacacca gcaccactgt gcacattcct
     8881 gtgacgctga ccggcccccc cggggagacc acggtagtgc ccagtggaaa cgtggacctg
     8941 gaaagtcaag cagaggggaa gaaggaggtg gaaaccagcg atgtgatgag gagcggccct
     9001 cggcccattg tcccgtacag ttccatgttc tgtttaagcc caaccaacct gctgcgccgc
     9061 tgctgccact acattgtgac catgcgatac ttcgagatgg tcatcctcgt ggtcatcgcc
     9121 ctgagcagca tcgccctggc tgccgaggac cccgtgagaa cagactctcc caggaataat
     9181 gctctgaagt acatggacta catcttcaca ggcgtcttca ccttcgagat ggtgataaag
     9241 atgatcgatt tgggactgct gctacaccct ggagcctatt tccgggatct gtggaacatt
     9301 ctggacttca ttgtggtcag tggggccctg gtggcatttg ctttctcagg atccaaaggg
     9361 aaagacatca gcaccatcaa atccctgaga gtcctgcgtg tgctgcggcc actcaagacc
     9421 atcaagcggc tgcccaagct caaggccgtg tttgactgtg tggtgaactc actgaaaaac
     9481 gtcctcaaca tcctgatcgt ctacatgctc ttcatgttca tctttgccgt cattgccgtg
     9541 cagctcttca aagggaagtt cttctactgc acggacgaat ccaaggagct ggagcgtgac
     9601 tgcaggggtc agtatttgga ttatgagaag gaggaagtgg aagctcaacc gaggcagtgg
     9661 aagaaatatg acttccacta tgacaacgtg ctctgggctc tgctaacgct cttcacggtg
     9721 tccacagggg aagggtggcc catggtgctg aaacactctg tggatgccac ctacgaggag
     9781 cagggcccga gccccgggta ccgcatggag ctctccatct tctacgtggt ctactttgtg
     9841 gtctttccct tcttctttgt caacatcttc gtggccttga tcatcatcac cttccaggag
     9901 cagggggaca aggtgatgtc cgagtgcagc ctggagaaga acgagagggc ttgcatcgac
     9961 tttgccatca gcgccagacc cctgacgcgg tacatgcccc agaacaagca gtcgttccag
    10021 tataagacgt ggacgttcgt ggtgtcccca ccttttgagt acttcatcat ggctatgatc
    10081 gccctcaaca ctgtggtgct gatgatgaag ttctacgatg cgccgtacga gtatgagcta
    10141 atgctgaaat gcctgaacat cgtgttcacg tccatgttct ccatggagtg tgtgctgaag
    10201 atcatcgcct tcggggtgct gaactatttc agagatgcct ggaatgtctt tgactttgtc
    10261 acggtgttgg gaagtattac tgatattttg gtaacagaga ttgcgaacaa cttcatcaac
    10321 ctcagcttcc tgcgcctgtt ccgggccgcg cggctcatca agctgctgcg ccagggctac
    10381 accatccgca tcctgctttg gaccttcgtg cagtccttca aggcgctgcc ctacgtgtgt
    10441 ctcctcatcg ccatgctctt cttcatctac gccatcattg gcatgcaggt ttttgggaac
    10501 atcgccctgg acgatgacac cagcatcaac aggcacaata acttccggac gtttctgcag
    10561 gccttgatgt tgctgttcag gagcgccacg ggggaggcct ggcacgagat aatgctgtcg
    10621 tgcctcagca gccgggcctg cgacgagcac tccaatgcca gcgagtgcgg cagcgacttt
    10681 gcctactttt acttcgtctc cttcatcttc ctctgctcct ttctgatgtt gaacctcttt
    10741 gtggctgtga tcatggacaa ttttgagtac cttacacggg actcttccat cttagggcct
    10801 caccacctgg atgagttcat ccgggtctgg gctgaatacg acccggccgc gtgtgggcgc
    10861 atcagttaca gtgacatgtt tgagatgctg aagcacatgt ccccacccct ggggctgggg
    10921 aagaagtgcc ctgctcgagt cgcgtacaag cgcctggtcc gcatgaacat gcccatctcc
    10981 agtgaggaca tgacggtgca cttcacatcc acgctgatgg ctctcattcg gacagcgctg
    11041 gacatcaagc tggcaccagc tgggaccaag cagcaccagt gcgacgcaga gctgaggaag
    11101 gagatttcct gtgtgtgggc caatctgccc cagaagaccc tggacttact ggtaccaccc
    11161 cataagcctg acgagatgac ggtgggcaaa gtctatgcag ctctgatgat cttcgacttc
    11221 tacaaacaga acaaaaccag cagagaccag actcagcaag cccccggagg cctgtcccag
    11281 ctgggccccg tgtccctgtt ccacccgctg aaggccacgc tggagcagac tcagccggcg
    11341 ctgcgtggag cccgggcttt cctgcggcag aagagttctg cctccctgag caacggcggg
    11401 gccgtacaaa cccaggagag cggcatcaag gaatcggttt cttggggcac tcagaggacc
    11461 caggatgtgc tttgcgaggc cagggcaccc ctggagcggg gccactcagc tgagatccca
    11521 gtcgggcagc ctgggacact ggctgtagat gtccagatgc agaacatgac tctgagtggc
    11581 cctgacgccg agccgcagcc agggctggag agccagggcc gagccgcctc catgccccgc
    11641 ctggccgcag aaacacagcc cgcgcctgac gccagcccca tgaagcgctc catctccacg
    11701 ctggccccgc gcccccacac ggcccggctc ggcagcaccg cgctggaccg cccagccccc
    11761 agccaggccc cgcaccatca ccaccaccgc tgccaccgcc gcagggacag gaagcagcgg
    11821 tccctggaga agggtcccag cctgtctgct gacacagatg gggcccccga cagcacggtg
    11881 gggccaggac tgcccacggg ggagggcccc cccggctgca ggcgggagcg ggagcgcagg
    11941 caggagcggg gccggtccca ggagcggagg cagccctcgt cctcctcctc ggagaagcat
    12001 cgcttctact cctgtgaccg cttcgggggc cgcgaacccc cacagcccaa gccctccctc
    12061 agtagtcacc ccacgtcgcc gacggccggg caggagccgg gaccccaccc gcaggggagt
    12121 ggttcggttc acgggagccc cctgctgtcc acatctggtg ctagcacccc gggccggggg
    12181 cggaggcagc tcccccagac ccccttgacc ccccgcccca gcgtcaccta caagacggcc
    12241 aactcctcgc ctgtccactt cgccggggca ccgagcggcc tccccgcctt ctcccccggc
    12301 cggctcagcc ggggcctctc agaacacaac gccctgctgc agagg
//
