LOCUS       sequence-415439-        5688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SoxS activation domain and gRNA targeting two copies of 
            106 region within the synthetic promoter in bacterial cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Naseri G, Raasch H, Charpentier E, Erhardt M
  TITLE     A versatile regulatory toolkit of arabinose-inducible artificial 
            transcription factors for Enterobacteriaceae.
  JOURNAL   Commun Biol. 2023 Oct 3;6(1):1005. doi: 10.1038/s42003-023-05363-3.
  PUBMED    37789111
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-10-3"; volume: "6(1):1005. doi"; pages: " 
            10.1038/s42003-023-05363-3"
COMMENT     Sequence Label: pSJ106-2x
FEATURES             Location/Qualifiers
     source          1..5688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     118..137
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        363..397
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        481..499
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     CDS             522..551
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             567..584
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(640..657)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      810..856
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(909..926)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1074..1168)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(1371..1398)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(1414..1485)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1518..2195)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             2202..2213
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(2223..2257)
                     /label=BBa_J23117
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.06"
     primer_bind     complement(2470..2487)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2470..2486)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2479..2501)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2695..2714)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2814..2836
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        2960..3064
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3065..3133,3134..3925)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3283..3302)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     RBS             4966..4977
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(5007..5291)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(5065..5084)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     rep_origin      join(5317..5688,1..217)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
       61 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      121 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      181 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
      241 acggttcctg gccttttgct ggccttttgc tctcagataa aatatttcta gatttcagtg
      301 caatttatct cttcaaatgt agcacctgaa gtcagcccca tacgatataa gttgttacta
      361 gattgacagc tagctcagtc ctaggtataa tactagtagg acgcctttgg taaccgcgtt
      421 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
      481 acatgaggat cacccatgtg ctttttttga agcttgggcc cgaacaaaaa ctcatctcag
      541 aagaggatct gaatagcgcc gtcgaccatc atcatcatca tcattgagtt taaacggtct
      601 ccagcttggc tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga
      661 acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc
      721 tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc
      781 ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      841 gggcctttcg ttttatctgt tgtttgtcgg tgaactggat ccttactcga gtctagactg
      901 caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      961 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1021 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aactagtgct tggattctca
     1081 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     1141 gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc
     1201 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
     1261 acaaacggca tgatgaacct gaatcgccag ccgagacgaa agggcctcgt gatacgccta
     1321 tttttatagg ttaatgtcat gataataatg gtttcttacc taggtataaa cgcagaaagg
     1381 cccacccgaa ggtgagccag tgtgactcta gtagagagcg ttcaccgaca aacaacagat
     1441 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tggagatcct
     1501 tactcgagtt tggatcctta agcaccggtg gagtgacgac cttcagcacg ttcgtactgt
     1561 tcaacgatgg tgtagtcttc gttgtgggag gtgatgtcca gtttgatgtc ggttttgtaa
     1621 gcacccggca gctgaaccgg ttttttagcc atgtaggtgg ttttaacttc agcgtcgtag
     1681 tgaccaccgt ctttcagttt cagacgcatt ttgatttcac ctttcagagc accgtcttcc
     1741 gggtacatac gttcggtgga agcttcccaa cccatggttt ttttctgcat aaccggaccg
     1801 tcggacggga agttggtacc acgcagttta actttgtaga tgaactcacc gtcttgcagg
     1861 gaggagtcct gggtaacggt aacaacacca ccgtcttcga agttcataac acgttcccat
     1921 ttgaaacctt ccgggaagga cagtttcagg tagtccggga tgtcagccgg gtgtttaacg
     1981 taagctttgg aaccgtactg gaactgcggg gacaggatgt cccaagcgaa cggcagcgga
     2041 ccacctttgg taactttcag tttagcggtc tgggtacctt cgtacggacg accttcacct
     2101 tcaccttcga tttcgaactc gtgaccgtta acggaacctt ccatacgaac tttgaaacgc
     2161 atgaactctt tgataacgtc ttcgctactc gccatggtac ctttctcctc tttaatgaat
     2221 tcgctagcac aatccctagg actgagctag ctgtcaagtc cggaggtatc cggtggaacc
     2281 caaaggacgc ctttggtaac cgcaggacgc ctttggtaac cgcaggacac cggtggtggc
     2341 ccatggtcac cataggtcac ccttggcaac caaaggtgtc ctatggcggc cggaggctgc
     2401 cataggtctc cggtggatac cgtaggcgac gtcatctaga tgcattcgcg aggtaccgag
     2461 ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     2521 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     2581 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     2641 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     2701 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     2761 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     2821 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     2881 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     2941 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3001 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3061 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     3121 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     3181 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     3241 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     3301 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     3361 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     3421 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     3481 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     3541 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     3601 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     3661 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3721 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3781 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3841 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3901 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     3961 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4021 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4081 gatcaaagga tcttcaagca gcataacctt tttccgtgat ggtaacttca cggtaaccaa
     4141 gatgtcgagt taaccaccct ttagattcat aaagcgaaaa taatgcggct ccaacgtacc
     4201 cacctaaatg gaaacggcgt tcactccaag cgaaaaaacc ccgccgaagc ggggtttttt
     4261 gcgttaaagc cggtgacggt agtcgctggg agtccggtca aattgacgag cgaagacgcg
     4321 agagaaggtt tgctgcgaca catagcctaa gtccatggca atatcgaaaa tcggacgttc
     4381 tgttgtccgc aattcaacgg cagccaacaa caaacggcgt tgacggatat agtctcctaa
     4441 tgtttgatgt gtgacggtac gaaacatgcg ctgtaaatac cacttcgagt aaccactttt
     4501 cttcgctaca acatcaatat taagaggctg atcaatatgc tcatcgatcc aagcaataag
     4561 gtcctggatg attttttggt ggctcattga accacctccg ccgtaaatac cactgttggc
     4621 cgcgatggca gatggaatcg gatttccatc tttcagaaga ccctgcatcg ctttcacaat
     4681 caactcacag tcgctattcg tagcaaagat cggaatagtc agttccatat taaggtacga
     4741 acgccatgca cctttaggga cttcaacttt gatcgtgtac ttacggtttt gcgcactcga
     4801 ttgacgtaca gaacaagtaa ctttgtatgc ttggctacgg ctgttggagg aaatccactc
     4861 ggcaatgccg ttagcgaagt tgctaggggc aacagttaca tcaccagtac caccattatc
     4921 cacaagaacg aactgcgtaa aattgctcgc gggacccata gatcctttct cctctttaga
     4981 tcttttgaat tcccaaaaaa acgggtatgg agaaacagta gagagttgcg ataaaaagcg
     5041 tcaggtagaa tccgctaatc ttatggataa aaatgctatg gcatagcaaa gtgtgacgcc
     5101 gtgcaaataa tcaatgtgga cttttctgcc gtgattatag acacttttgt tacgcgtttt
     5161 tgtcatggct ttggtcccgc tttgttacag aatgctttta ataagcgggg ttaccggttt
     5221 ggttagcgag aagagccagt aaaagacgca gtgacggcaa tgtctgatgc aatatggaca
     5281 attggtttct tctctgaatg gcgggagtat gaaaagttga gatccttttt ttctgcgcgt
     5341 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5401 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5461 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5521 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5581 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5641 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaact
//
